本文關(guān)鍵詞： 急性肺栓塞 骨髓間充質(zhì)干細胞 細胞治療 連續(xù)切片 性別差異 急性肺栓塞 甘油醛-3-磷酸脫氫酶 骨髓間充質(zhì)干細胞　出處：《華中科技大學(xué)》2013年博士論文　論文類型：學(xué)位論文

【摘要】：急性肺栓塞是臨床常見疾病,具有高發(fā)病率、高死亡率、高致殘率,以及臨床表現(xiàn)的多樣化,成為治療的難點。目前,針對肺栓塞的治療措施以抗凝治療及溶栓治療為主。但是藥物治療存在著特異性差、半衰期短、副作用大等缺點。干細胞治療疾病的前景令人鼓舞,但目前的研究就其是否能夠促進血栓溶解還存在爭議,并且骨髓間充質(zhì)干細胞對急性肺栓塞的治療是否有效還未得到證實。本研究利用頸外靜脈注射血栓復(fù)制急性肺栓塞動物模型,然后用骨髓間充質(zhì)干細胞進行治療,發(fā)現(xiàn)治療有效。為評估治療效果,在本研究中我們首創(chuàng)應(yīng)用全肺連續(xù)切片的方法統(tǒng)計實際存在的每個栓子的體積,并且與傳統(tǒng)的非連續(xù)切片統(tǒng)計血栓面積的方法進行比較,證實了對肺栓塞這種多血栓的情況進行定量評估時利用全肺連續(xù)切片的必要性。 多項研究報道細胞存在性別差異,且發(fā)現(xiàn)干細胞在治療多種疾病都表現(xiàn)為雌性干細胞比雄性干細胞更有優(yōu)勢。利用干細胞治療血栓的報道很多,但都未探討性別差異的問題。本研究首次探討不同性別的骨髓間充質(zhì)干細胞對急性肺栓塞的治療效果,發(fā)現(xiàn)雌性骨髓間充質(zhì)干細胞具有更優(yōu)于雄性干細胞的治療效果。GAPDH作為一種管家蛋白除了參與糖酵解外,還參與包括DNA修復(fù)、tRNA轉(zhuǎn)運、膜融合轉(zhuǎn)運、細胞骨架構(gòu)建甚至細胞死亡等多種細胞生命活動的調(diào)節(jié)。有學(xué)者對大鼠腦部的研究發(fā)現(xiàn)GAPDH的表達存在性別差異。我們的實驗也證明正是雌、雄骨髓間充質(zhì)干細胞的GAPDH表達差異造成了其對急性肺栓塞的治療差異,具有較高GAPDH基礎(chǔ)水平的雌性干細胞對急性肺栓塞的治療效果更好,而GAPDH的這一作用,可能是通過調(diào)控尿激酶型纖溶酶原激活物來實現(xiàn)的。 目的 觀察不同性別骨髓間充質(zhì)干細胞對急性肺栓塞的治療作用。 方法 利用小鼠頸外靜脈注射血栓復(fù)制急性肺栓塞的動物模型。造模后4h、8h及16h分組經(jīng)尾靜脈注射不同性別的骨髓間充質(zhì)干細胞2×106個,造模24h后處死小鼠,取肺固定包埋。全肺連續(xù)切片,HE染色,精確統(tǒng)計出現(xiàn)的所有栓子,并通過體積計算方法分析治療差異。 結(jié)果 1.成功復(fù)制急性肺栓塞的動物模型 模型組經(jīng)過血栓注射后24小時,通過切片染色可以看到非常明顯的血栓。利用連續(xù)切片體積分析方法對模型組和治療組所有的栓子進行分析,栓子體積主要集中于0.1-5×106μm3,利用非連續(xù)切片面積分析方法分析栓子面積主要位于1-10x103μm2間,兩種分析方法都顯示栓子在肺內(nèi)的分布右肺多于左肺,且主要位于0-30μm直徑的小血管內(nèi),這與臨床血栓肺內(nèi)分布基本一致。 2.用總體積比用總面積分析多發(fā)血栓更加科學(xué)、合理；雌性和雄性骨髓間充質(zhì)干細胞均對急性肺栓塞有治療效果,且雌性骨髓間充質(zhì)干細胞更有優(yōu)勢。 用文獻常用的面積計算方法來分析模型動物肺血栓情況發(fā)現(xiàn),較連續(xù)切片體積計算法血栓總數(shù)漏掉了54%,但栓子的分布特點和臨床基本一致。用連續(xù)切片體積來分析,統(tǒng)計所有出現(xiàn)的栓子,更加精確和客觀。栓塞4h后治療,在雌性小鼠和雄性小鼠中,雌性和雄性骨髓間充質(zhì)干細胞均有效果,且雌性骨髓間充質(zhì)干細胞治療效果更好；栓塞8h后治療,對于雌性小鼠,雌、雄骨髓間充質(zhì)干細胞均有效果,但未發(fā)現(xiàn)兩者的治療差異,而在雄性小鼠,雌、雄骨髓間充質(zhì)干細胞均有效果,且雌性骨髓間充質(zhì)干細胞治療效果更好；栓塞16h后治療,雌、雄骨髓間充質(zhì)干細胞對雌性小鼠均有效果,但兩者未發(fā)現(xiàn)治療差異,而在雄性小鼠,僅僅雌性骨髓間充質(zhì)干細胞治療有效。 結(jié)論 利用頸外靜脈注入血栓法可以成功復(fù)制急性肺栓塞動物模型,復(fù)制的模型與臨床資料肺血栓的分布基本一致,用這種復(fù)制血栓的方法來研究肺栓塞是可行可靠的。相對于文獻常用面積計算分析血栓的方法,我們獨創(chuàng)全肺連續(xù)切片計算血栓體積來分析的方法更加準(zhǔn)確和客觀。雌、雄骨髓間充質(zhì)干細胞在肺血栓發(fā)生的早期治療均有效,且雌性骨髓間充質(zhì)干細胞治療效果更好；而在血栓發(fā)生的晚期,僅僅雌性骨髓間充質(zhì)干細胞治療有效。 目的 確定GAPDH在調(diào)控雌、雄骨髓間充質(zhì)干細胞治療急性肺栓塞效果差異中的作用,進一步探討骨髓間充質(zhì)干細胞治療急性肺栓塞的機制。 方法 1.雌性骨髓間充質(zhì)干細胞穩(wěn)轉(zhuǎn)GAPDH干擾的質(zhì)粒或空質(zhì)粒,雄性骨髓間充質(zhì)干細胞穩(wěn)轉(zhuǎn)GAPDH過表達的質(zhì)�；蚩召|(zhì)粒,western blotting檢測各細胞中GAPDH的表達情況。 2.復(fù)制急性肺栓塞的動物模型。栓塞后4h分組注射調(diào)控了GAPDH表達的骨髓間充質(zhì)干細胞(F-BMSCs-siRNA-GAPDH、F-BMSCs-siRNA vector、 M-BMSCs OE-GAPDH、M-BMSCs OE vector)2×106個,20h后處死小鼠,取肺固定包埋。全肺連續(xù)切片,HE染色,精確統(tǒng)計出現(xiàn)的所有栓子,分析治療差異。 3.全肺連續(xù)切片中所有奇數(shù)片HE染色,發(fā)現(xiàn)有連續(xù)的片子出現(xiàn)血栓,其對應(yīng)的偶數(shù)片子做免疫熒光,對干細胞進行追蹤；western blotting檢測各細胞中uPA的表達情況。 結(jié)果 1. GAPDH穩(wěn)定轉(zhuǎn)染的情況 未處理的雌性骨髓間充質(zhì)干細胞中GAPDH的基礎(chǔ)表達量要高于雄性骨髓間充質(zhì)干細胞；雌性骨髓間充質(zhì)干細胞轉(zhuǎn)染干擾GAPDH的質(zhì)粒后,其GAPDH表達量明顯減少；雄性骨髓間充質(zhì)干細胞轉(zhuǎn)染GAPDH過表達的質(zhì)粒后,其GAPDH表達量明顯增高。 2. GAPDH轉(zhuǎn)染后的骨髓間充質(zhì)干細胞對急性肺栓塞的治療情況 急性肺栓塞4h后開始干細胞治療,干擾GAPDH表達的雌性骨髓間充質(zhì)干細胞治療組的血栓體積明顯大于單純雌性骨髓間充質(zhì)干細胞治療組,而GAPDH過表達的雄性骨髓間充質(zhì)干細胞治療組比單純雄性骨髓間充質(zhì)干細胞治療組血栓體積明顯減少,治療效果明顯增強。 3.干細胞追蹤及uPA表達情況 免疫熒光實驗發(fā)現(xiàn)綠色熒光標(biāo)記的骨髓間充質(zhì)干細胞主要在血管內(nèi)栓子的外緣附著。雌性骨髓間充質(zhì)干細胞中uPA表達量高于雄性骨髓間充質(zhì)干細胞,GAPDH過表達以后的骨髓間充質(zhì)干細胞uPA表達量明顯增高。 結(jié)論 雌性骨髓間充質(zhì)干細胞具有比雄性骨髓間充質(zhì)干細胞更好地對肺血栓的治療效果,其原因可能是雌性骨髓間充質(zhì)干細胞中具有顯著的高于雄性骨髓間充質(zhì)干細胞的GAPDH表達量。通過干預(yù)GAPDH在雌雄骨髓間充質(zhì)干細胞的表達更驗證了雌、雄骨髓間充質(zhì)干細胞中GAPDH表達水平的不同是造成這兩種干細胞對急性肺栓塞治療差異的原因。 干細胞在血栓外緣的定位以及骨髓間充質(zhì)干細胞中GAPDH可以調(diào)控uPA的表達,說明急性肺栓塞的早期骨髓間充質(zhì)干細胞可能主要通過分泌一些溶栓因子來促進血栓的溶解,從而起到治療作用。
[Abstract]:Acute pulmonary embolism is a common clinical disease with high morbidity, high mortality, high morbidity, and varied clinical manifestations, become the treatment difficulty. At present, the pulmonary embolism treatment measures to anticoagulant therapy and thrombolytic therapy. But the drugs have poor specificity, short half-life, side effects and other shortcomings stem cells in the treatment of disease. The prospects are encouraging, but the current study is whether it can promote thrombolysis is still controversial, and bone marrow mesenchymal stem cells in treatment of acute pulmonary embolism is effective. This study has not been confirmed by external jugular vein injection of thrombus in animal model of acute pulmonary embolism, and bone marrow mesenchymal stem cells for treatment, that treatment is effective. In order to evaluate the therapeutic effect, in this study we initiated each sub application of statistical methods of whole lung thrombus in serial sections of the actual volume, Compared with the traditional method of non continuous slice statistics for thrombus area, it is confirmed that the necessity of whole lung continuous slice is used for quantitative evaluation of pulmonary thromboembolism.
There are gender differences reported in many studies and found that cells, stem cells in the treatment of various diseases are expressed as female cells than male stem cells have more advantages. The use of stem cells in the treatment of thrombosis reported, but did not investigate the gender differences. This is the first study to explore the gender therapeutic effect of bone marrow mesenchymal stem cells on acute pulmonary embolism, found that female bone marrow mesenchymal stem cells is more than the male dry treatment effect of.GAPDH cells as a housekeeping protein in addition to glycolysis, also involved in DNA repair, tRNA transporter, membrane fusion transporter, regulate cytoskeletal structure and even cell death and other cellular activities. Some scholars the rat brain found gender differences in the expression of GAPDH. Our experiments have shown that it is female, male bone marrow mesenchymal stem cells which are caused by the differences in the expression of GAPDH on acute The treatment of pulmonary embolism is different from that of female stem cells with a higher level of GAPDH, which is better for the treatment of acute pulmonary embolism. The role of GAPDH may be achieved by regulating urokinase type plasminogen activator.
objective
To observe the therapeutic effect of different sex bone marrow mesenchymal stem cells (MSCs) on acute pulmonary embolism.
Method
Using a mouse external jugular vein injection of thrombus replication of acute pulmonary embolism animal model. After modeling, 4h, 8h and 16h in bone marrow and divided into different groups by intravenous injection of sex mesenchymal stem cells 2 * 106 24h after modeling, the mice were killed, the lung paraffin embedded lung. Serial sections, HE staining, all an accurate statistics appear, and the volume calculation method analysis of treatment differences.
Result
1. animal model of successfully replicating acute pulmonary embolism
The model group after 24 hours after injection of thrombus, can see very obvious thrombosis by slicing staining. Analysis method of model group and treatment group all emboli were analyzed by serial sections of volume, volume of emboli mainly focused on 0.1-5 * 106 m3, using the analysis method of non continuous slice area analysis of embolus area mainly located in 1-10x103 m2. Two, analysis methods are shown in the distribution of pulmonary emboli than the right lung left lung, small blood vessels and mainly located in 0-30 m in diameter, and the clinical thrombosis pulmonary distribution is basically the same.
2., it is more scientific and reasonable to analyze multiple thrombus with total volume ratio and total area. Both female and male bone marrow mesenchymal stem cells have therapeutic effect on acute pulmonary embolism, and female bone marrow mesenchymal stem cells have more advantages.
To analyze the animal model of pulmonary embolism was found in the literature area commonly used calculation method, a continuous slice volume calculation method of thrombus total missing 54%, but the distribution and clinical characteristics of emboli are basically the same. The serial sections were used to analyze the volume, statistics of all the emboli, more accurate and objective. 4h after embolization treatment in female mice male and female and male mice, bone marrow mesenchymal stem cells have the effect, and the female bone marrow mesenchymal stem cells to better treatment effect; embolism after 8h treatment for the female mice, female and male bone marrow mesenchymal stem cells have the effect, but did not find the difference between the two treatment, while in male mice, female male, bone marrow mesenchymal stem cells have the effect, and the female bone marrow mesenchymal stem cells to better treatment effect; estrogen therapy, embolism after 16h male bone marrow mesenchymal stem cells of female mice were effective, but they were not found The treatment was different, but in male mice, only female bone marrow mesenchymal stem cells were treated effectively.
conclusion
Successfully established an animal model of acute pulmonary embolism by external jugular vein injection of thrombus, the distribution model of reproduction and clinical data of pulmonary embolism are basically the same, the methods used in this research to copy thrombosis pulmonary embolism is feasible and reliable. Compared to the commonly used literature area calculation method analysis of blood thrombus, we original whole lung sections calculation to analyze the thrombus volume accurately and objectively. The female and male bone marrow mesenchymal stem cells were effective in the early treatment of pulmonary thromboembolism, and female bone marrow mesenchymal stem cells and better treatment effect; in late thrombosis, only female bone marrow mesenchymal stem cells treatment is effective.
objective
Objective to determine the role of GAPDH in regulating the difference between male and female bone marrow mesenchymal stem cells in the treatment of acute pulmonary embolism, and further explore the mechanism of bone marrow mesenchymal stem cells in the treatment of acute pulmonary embolism.
Method
1., the bone marrow mesenchymal stem cells were transferred to GAPDH interference plasmid or empty plasmid. Male bone marrow mesenchymal stem cells stably transferred to GAPDH over expressed plasmid or empty plasmid. Western blotting was used to detect GAPDH expression in all cells.
2. animal models of replication of acute pulmonary embolism after embolization. The regulation of the expression of GAPDH 4H packet injection of bone marrow mesenchymal stem cells (F-BMSCs-siRNA-GAPDH, F-BMSCs-siRNA vector, M-BMSCs OE-GAPDH, M-BMSCs OE vector) 2 * 106 20h, mice were sacrificed and lung paraffin embedded lung. Serial sections, HE staining, all. The precise statistics, analysis of treatment differences.
3. in all lung continuous sections, all the odd HE stains were found. There was continuous thrombus in the slices. The corresponding even slices were immunofluorescent, and the stem cells were tracked. Western blotting was used to detect the expression of uPA in all the cells.
Result
1. GAPDH stable transfection
The untreated female bone marrow mesenchymal stem cells based on the expression of GAPDH is higher than that of male bone marrow mesenchymal stem cells; female bone marrow mesenchymal stem cells transfected with plasmid GAPDH, the expression of GAPDH was significantly reduced; male bone marrow mesenchymal stem cells transfected with GAPDH expression plasmid, GAPDH the expression is significantly increased.
Treatment of acute pulmonary embolism by 2. GAPDH transfected bone marrow mesenchymal stem cells
Stem cell therapy to acute pulmonary embolism after 4H interference GAPDH expression of female bone marrow mesenchymal stem cells in the treatment group was significantly higher than that of pure thrombus volume female bone marrow mesenchymal stem cells for the treatment group, while the expression of GAPDH male bone marrow mesenchymal stem cells in the treatment group were significantly reduced compared with male bone marrow mesenchymal stem cell therapy group thrombosis volume, treatment effect is significantly enhanced.
3. stem cell tracking and uPA expression
Immunofluorescence experiments showed that outer green fluorescent labeled bone marrow mesenchymal stem cells in intravascular emboli attachment. Female bone marrow mesenchymal stem was higher than that of male bone marrow mesenchymal stem cells uPA cells, overexpression of GAPDH after bone marrow mesenchymal stem cells uPA expression was significantly increased.
conclusion
Female bone marrow mesenchymal stem cells compared with male bone marrow mesenchymal stem cells on pulmonary thrombosis and better treatment effect, which may be due to female bone marrow mesenchymal stem cells is significantly higher than that of male bone marrow mesenchymal stem cells GAPDH expression of mesenchymal stem cells. The expression was verified by more female intervention the GAPDH charge in male and female bone marrow, male bone marrow mesenchymal stem cells in the expression level of GAPDH is different from the two kinds of stem cells in the treatment of acute pulmonary embolism and the reasons for the differences.
The location of stem cells in the outer edge of thrombus and the expression of uPA in bone marrow mesenchymal stem cells can regulate the expression of uPA, indicating that early bone marrow mesenchymal stem cells in acute pulmonary embolism may play a therapeutic role by promoting the dissolution of thrombus by secreting some thrombolytic factors.

【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2013
【分類號】：R563.5

【相似文獻】

相關(guān)期刊論文 前10條

1 胡秀紅,劉玉愛,邱振美;術(shù)中發(fā)生急性肺栓塞1例的護理教訓(xùn)[J];齊魯護理雜志;2001年02期

2 孫銀翠 ,譚利玲 ,于曉麗;骨折后致急性肺栓塞護理體會[J];中醫(yī)正骨;2001年11期

3 宋延強,呂文平;老年胃腸惡性腫瘤術(shù)后急性肺栓塞17例[J];濱州醫(yī)學(xué)院學(xué)報;2002年04期

4 洪霞;;肺栓塞的診斷[J];臨床肺科雜志;2006年03期

5 李新;;肺栓塞32例臨床分析[J];中國冶金工業(yè)醫(yī)學(xué)雜志;2006年02期

6 呂蕾;;急性肺栓塞22例臨床分析[J];醫(yī)學(xué)理論與實踐;2006年06期

7 李];辛雨玲;馬秋波;劉麗麗;;肺栓塞42例臨床心電圖分析[J];河北醫(yī)藥;2008年03期

8 馬理華;段建明;安新;;急性非大面積肺栓塞6例抗凝治療體會[J];臨床肺科雜志;2011年05期

9 朱松山;;肺栓塞病的診斷和治療[J];國外醫(yī)學(xué).內(nèi)科學(xué)分冊;1977年03期

10 王文中;肺栓塞的診斷[J];中國醫(yī)師進修雜志;1989年07期

相關(guān)會議論文 前10條

1 虞希祥;《子宮肌瘤病理血管徹底性栓塞治療的臨床研究》課題組;;大面積急性肺栓塞介入治療與預(yù)防的臨床研究[A];醫(yī)學(xué)科研成果與應(yīng)用[C];2011年

2 朱華;張麗;匡名洋;;23例胃腸道惡性腫瘤術(shù)后急性肺栓塞的臨床分析及護理[A];全國外科護理學(xué)術(shù)交流暨專題講座會議、全國神經(jīng)內(nèi)、外科護理學(xué)術(shù)交流暨專題講座會議論文匯編[C];2010年

3 王勇;張洪亮;趙智慧;羅勤;趙青;柳志紅;;惡性腫瘤與急性肺栓塞遠期死亡風(fēng)險的關(guān)系[A];中國心臟大會（CHC）2011暨北京國際心血管病論壇論文集[C];2011年

4 程顯聲;;中危急性肺栓塞溶栓療法——我們的建議[A];呼吸與危重癥醫(yī)學(xué)（2010-2011）[C];2011年

5 費曉鶯;鄭素婷;范愛敏;;骨折病人術(shù)后發(fā)生急性肺栓塞的分析與對策[A];創(chuàng)建患者安全文化——中華護理學(xué)會第15屆全國手術(shù)室護理學(xué)術(shù)交流會議論文匯編（中冊）[C];2011年

6 朱莎莎;祁建勇;張曉璇;張敏州;;中西醫(yī)結(jié)合成功搶救急性肺栓塞合并心跳呼吸驟停患者的護理體會1例[A];首屆全國中西醫(yī)結(jié)合重癥醫(yī)學(xué)學(xué)術(shù)會議暨中國中西醫(yī)結(jié)合學(xué)會重癥醫(yī)學(xué)專業(yè)委員會成立大會論文匯編[C];2010年

7 劉艷;胡斌;毛毅敏;;高半胱氨酸血癥合并急性肺栓塞(兔)影像動物模型的建立[A];中華醫(yī)學(xué)會第九次全國核醫(yī)學(xué)學(xué)術(shù)會議論文摘要匯編[C];2011年

8 李凝;王鐵;;核素肺顯像對急性肺栓塞伴右心功能不全溶栓前后療效研究[A];中華醫(yī)學(xué)會第九次全國核醫(yī)學(xué)學(xué)術(shù)會議論文摘要匯編[C];2011年

9 陳勇;劉雙;郭偉;王增智;;心臟型脂肪酸結(jié)合蛋白對入院時血流動力學(xué)穩(wěn)定的急性肺栓塞患者短期預(yù)后的評估[A];中華醫(yī)學(xué)會呼吸病學(xué)年會——2011（第十二次全國呼吸病學(xué)學(xué)術(shù)會議）論文匯編[C];2011年

10 王勇;張洪亮;趙智慧;羅勤;趙青;柳志紅;;特發(fā)性急性肺栓塞遠期復(fù)發(fā)風(fēng)險的臨床分析[A];中國心臟大會（CHC）2011暨北京國際心血管病論壇論文集[C];2011年

相關(guān)重要報紙文章 前10條

1 羅學(xué)宏　(中南大學(xué)湘雅醫(yī)院急診科 教授);急性肺栓塞與惡性腫瘤[N];醫(yī)藥經(jīng)濟報;2010年

2 本報記者 李廣宇;溶栓抗凝,急性肺栓塞可控[N];醫(yī)藥經(jīng)濟報;2011年

3 本報記者 曾令浩;急性肺栓塞：中�；颊呷芩ǖ臎Q擇[N];醫(yī)藥經(jīng)濟報;2011年

4 北京大學(xué)第一附屬醫(yī)院心內(nèi)科 陳明;需要高度重視的頑疾——急性肺栓塞[N];中國高新技術(shù)產(chǎn)業(yè)導(dǎo)報;2001年

5 衣曉峰 岳金鳳;機械旋轉(zhuǎn)疏通急性肺栓塞[N];中國醫(yī)藥報;2005年

6 記者 衣曉峰;急性肺栓塞猝死與縱軸突變有關(guān)[N];健康報;2001年

7 中南大學(xué)湘雅醫(yī)院急診科教授 鄧躍林;面對肺栓塞的挑戰(zhàn):急診醫(yī)師該做什么？[N];健康報;2007年

8 孫揚;急診取栓治療急性肺栓塞[N];中國醫(yī)藥報;2004年

9 陳德芝;肺栓塞類型不同治法各異[N];健康報;2007年

10 記者 許寧生;急性心梗溶栓和急性肺栓塞診療達成共識[N];醫(yī)藥經(jīng)濟報;2009年

相關(guān)博士學(xué)位論文 前10條

1 彭小春;骨髓間充質(zhì)干細胞性別差異對急性肺栓塞治療效果的影響及機制[D];華中科技大學(xué);2013年

2 丁筱雪;QT離散度在急性肺栓塞中的臨床意義[D];中南大學(xué);2012年

3 馬展鴻;肺栓塞的影像診斷學(xué)研究[D];中國協(xié)和醫(yī)科大學(xué);2004年

4 方緯;肺栓塞的核素顯像實驗研究與臨床應(yīng)用[D];中國協(xié)和醫(yī)科大學(xué);2003年

5 侯海軍;速度向量成像技術(shù)評估右室功能的研究[D];中國人民解放軍軍醫(yī)進修學(xué)院;2008年

6 叢志斌;超聲心動圖診斷兔急性肺栓塞價值的實驗研究[D];中國醫(yī)科大學(xué);2009年

7 倪松石;肺血栓栓塞癥早期血液標(biāo)志物變化及溶栓后肺組織缺血再灌注損傷研究[D];蘇州大學(xué);2008年

8 張燕;多層螺旋CT肺動脈造影聯(lián)合下肢靜脈造影診斷肺栓塞和深靜脈血栓的臨床研究[D];中國協(xié)和醫(yī)科大學(xué);2004年

9 路軍良;國產(chǎn)機械祛栓器械治療急性肺動脈栓塞的實驗研究[D];中國協(xié)和醫(yī)科大學(xué);2007年

10 靳建軍;辛伐他汀對大鼠急性肺栓塞的保護作用及其機制的研究[D];北京協(xié)和醫(yī)學(xué)院;2011年

相關(guān)碩士學(xué)位論文 前10條

1 徐術(shù)根;兩種評分系統(tǒng)對急性肺栓塞病情嚴(yán)重程度及預(yù)后評估的臨床研究[D];中南大學(xué);2011年

2 褚亞紅;老年人與非老年人急性肺栓塞的臨床比較[D];新疆醫(yī)科大學(xué);2011年

3 王洋;不典型急性肺栓塞1例報告及早期診斷線索回顧性分析[D];山東大學(xué);2012年

4 宣向飛;55例急性肺栓塞患者臨床分析[D];延安大學(xué);2013年

5 盛丹丹;急性肺栓塞臨床病例分析[D];石河子大學(xué);2013年

6 于養(yǎng)生;急性肺栓塞大鼠肝細胞生長因子的變化[D];桂林醫(yī)學(xué)院;2010年

7 卞淼;513例急性肺栓塞患者臨床特征及預(yù)后相關(guān)因素分析[D];寧夏醫(yī)科大學(xué);2010年

8 熊國均;臨床評分、D-二聚體檢測對急性肺栓塞的診斷價值[D];天津醫(yī)科大學(xué);2011年

9 梁春坡;血流動力學(xué)穩(wěn)定的急性肺栓塞患者預(yù)后分析[D];河北醫(yī)科大學(xué);2012年

10 駱偉娟;慢性阻塞性肺疾病并發(fā)急性肺栓塞12例臨床分析[D];浙江大學(xué);2010年

，

本文編號：1531388