本文關鍵詞：結核分枝桿菌抗原特異性白細胞介素-22在結核性胸膜炎鑒別診斷中的作用研究　出處：《中國人民解放軍醫(yī)學院》2014年碩士論文　論文類型：學位論文

  更多相關文章： 結核桿菌特異性白細胞介素-22 結核性胸水 惡性胸水 鑒別診斷

【摘要】：目的:通過分析結核性與惡性胸液單個核細胞（Pleural fluid mononuclear cells,PFMCs）在受到結核分枝桿菌特異性抗原肽ESAT-6（early secreted antigenic target-6）和CFP-10（culture filtrate protein-10）刺激后表達白細胞介素-22（Interleukin-22，IL-22）的特點，探索結核性和惡性胸水的鑒別診斷新方法。 方法：采用e-Bioscience的IL-22和干擾素-γ（Interferon-γ，IFN-γ）試劑盒和流式細胞術微球陣列法，對52例結核性胸水和35例惡性胸水中經(jīng)結核分枝桿菌特異性抗原肽刺激后PFMCs所表達的IL-22和IFN-γ濃度進行測定，并作統(tǒng)計學分析。 結果:經(jīng)結核桿菌特異抗原肽刺激，，結核性胸水上清中PFMC的IL-22表達水平顯著升高（P＜0.05），且高于惡性胸水（P＜0.05）。結核性胸膜炎的結核桿菌特異性IL-22在胸液上清PFMC中的表達水平與IFN-γ顯著相關(相關系數(shù)r=0.3485，P=0.0320)。ROC曲線顯示，結核桿菌特異性IL-22用于結核性胸膜炎的診斷敏感性、特異性和準確性分別為90.38%、80.56%和85.23%，其診斷效能與IFN-γ相當(P＞0.05)。 結論：結核分枝桿菌抗原特異性IL-22可作為鑒別診斷胸腔積液的新指標。
[Abstract]:Objective : To investigate the characteristics of interleukin - 22 ( IL - 22 ) after stimulation of Mycobacterium tuberculosis specific antigen peptide ( ESAT - 6 ) and P - 10 ( culture filtrate protein - 10 ) by analyzing tuberculous and malignant pleural fluid mononuclear cells ( PFs ) , and to explore new methods for differential diagnosis of tuberculous and malignant pleural effusion . Methods : The concentrations of IL - 22 and IFN - 緯 in 52 cases of tuberculous pleural effusion and 35 cases of malignant pleural effusion were measured with e - bioscience IL - 22 and interferon - 緯 ( interferon - 緯 , IFN - 緯 ) kit and flow cytometry . Results : The level of IL - 22 expression of PFMC was significantly increased ( P & lt ; 0.05 ) in the tuberculous pleural fluid by Mycobacterium tuberculosis specific antigen peptide stimulation ( P & lt ; 0.05 ) , and the expression level of TB - specific IL - 22 in pleural fluid supernatant was significantly correlated with IFN - 緯 ( r = 0.3485 , P = 0.0320 ) . ROC curves showed that the diagnostic sensitivity , specificity and accuracy of mycobacterium tuberculosis specific IL - 22 were 90.38 % , 80.56 % and 85.23 % , respectively , and their diagnostic efficacy was comparable with that of IFN - 緯 ( P > 0.05 ) . Conclusion : Mycobacterium tuberculosis antigen - specific IL - 22 can be used as a new index for differential diagnosis of pleural effusion .

【學位授予單位】：中國人民解放軍醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R521.7

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