本文關鍵詞：茶黃素納米粒霧化吸入對大鼠炎性氣道黏蛋白5AC的調控　出處：《重慶醫(yī)科大學學報》2016年07期 　論文類型：期刊論文

  更多相關文章： 茶黃素 黏蛋白AC 納米粒 霧化吸入

【摘要】：目的:探討高頻霧化吸入茶黃素乳酸-羥基乙酸共聚物(poly lactic-co-glycolic acid,PLGA)納米粒對炎性氣道黏蛋白5AC(mucoprotein 5AC,MUC5AC)的作用。方法:通過改良法制備茶黃素PLGA納米粒,用粒徑分析儀檢測納米粒平均粒徑,并進行包封率和載藥量測定及體外釋放試驗。將SD大鼠分為3組:對照組(A組)、香煙吸入組(B組)及干預組(C組)。RT-PCR檢測各組黏蛋白5AC m RNA含量;酶聯(lián)免疫吸附測定法(ELISA)觀察MUC5AC蛋白表達的變化,并進行組化對比大鼠氣道組織黏蛋白變化情況。結果:制備的茶黃素PLGA納米粒性質穩(wěn)定,測得其的平均粒徑為(108.24±2.86)nm,適宜霧化吸入。香煙吸入組MUC5AC的蛋白表達明顯升高,較對照組有顯著性差異(P=0.000),而給予茶黃素PLGA納米粒處理后,與香煙組比,指標明顯下調(P=0.001)。MUC5AC m RNA表達的RT-PCR電泳結果也有類似結果。氣道組織化學PAS染色也提示霧化吸入茶黃素PLGA納米粒后,氣道表面黏蛋白表達顯著下降。結論:通過改良法制備茶黃素PLGA納米粒性質穩(wěn)定,大小均勻;經(jīng)霧化途徑吸入可發(fā)揮抑制炎性氣道的黏蛋白5AC的高分泌作用,為茶黃素的開發(fā)應用提供了新的途徑。
[Abstract]:Objective: to study the high frequency atomization inhalation of theaflavin-lactic acid-glycolic acid copolymer poly lactic-co-glycolic acid. The effect of PLGA nanoparticles on inflammatory airway mucoprotein 5ACmucoprotein 5ACMUC5AC. Methods: theaflavin PLGA nanoparticles were prepared by modified method. The average particle size was measured by particle size analyzer, encapsulation efficiency, drug loading and in vitro release test were performed. SD rats were divided into three groups: control group A). The content of 5AC m RNA was detected by RT-PCR in group C and group C in cigarette inhalation group (group B) and intervention group (group C). Enzyme linked immunosorbent assay (Elisa) was used to observe the expression of MUC5AC protein. Results: the properties of theaflavine PLGA nanoparticles were stable and the average diameter was 108.24 鹵2.86 nm. The expression of MUC5AC protein in the cigarette inhalation group was significantly higher than that in the control group (P < 0.05), and the protein expression in the cigarette inhalation group was significantly higher than that in the control group (P < 0.05). However, after the treatment of theaflavin PLGA nanoparticles. Compared to cigarettes. Index significantly downgraded P0. 001). The results of RT-PCR electrophoresis of MUC5AC m RNA were similar. The airway histochemical PAS staining also suggested that the aerosol inhalation of theaflavin PLGA nanoparticles. Conclusion: the properties of theaflavine PLGA nanoparticles prepared by modified method are stable and uniform. Inhaled by atomization pathway can inhibit the hypersecretion of mucin 5AC in inflammatory airway, which provides a new way for the development and application of theaflavine.
【作者單位】： 重慶市中醫(yī)院呼吸科;
【基金】：重慶市科委基金資助項目(編號:cstc2013jcyjA1062)
【分類號】：R56
【正文快照】： 慢性氣道炎癥是導致支氣管哮喘、慢性阻塞性肺疾病(chronic obstructive pulmonary diseases,COPD)等臨床常見呼吸系統(tǒng)疾病的主要病理基礎。長期的炎癥反應損傷氣道上皮,進而使氣道上皮杯狀細胞、黏膜下腺體增生,纖毛損傷,黏液分泌增多,黏液屏障破壞,引起氣流阻塞,逐漸形成COP，

本文編號：1380867