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  本文關(guān)鍵詞：HLA-DQA1基因甲基化與維吾爾族飼鴿者肺相關(guān)性分析　出處：《石河子大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： HLA-DQA1基因 飼鴿者肺 DNA甲基化

【摘要】：目的:通過檢測(cè)維吾爾族飼鴿者肺外周血HLA-DQA1基因甲基化程度,并探討其意義。方法:以20例確診維吾爾族飼鴿者肺患者為病例組,20例維吾爾族飼養(yǎng)鴿子未發(fā)病者為陰性對(duì)照組,20例維吾爾族未飼養(yǎng)鴿子健康體檢者為正常對(duì)照組。收集資料并抽取外周血標(biāo)本,常規(guī)提取DNA、亞硫酸氫鹽轉(zhuǎn)化、PCR擴(kuò)增、體外轉(zhuǎn)錄和RNase A特異性酶切,并行基質(zhì)輔助激光解吸附電離飛行時(shí)間質(zhì)譜分析技術(shù)檢測(cè)HLA-DQA1基因甲基化情況。然后用spss17.0軟件進(jìn)行統(tǒng)計(jì)學(xué)分析,比較三組研究對(duì)象HLA-DQA1基因甲基化率是否存在差異,然后三組間兩兩比較HLA-DQA1甲基化率是否存在差異。結(jié)果:檢測(cè)HLA-DQA1片段的Cp G位點(diǎn)總數(shù)4個(gè)(Cp G_1,Cp G_2,Cp G_3,Cp G_4),實(shí)際檢測(cè)到的Cp G位點(diǎn)數(shù)3個(gè)(Cp G_2,Cp G_3,Cp G_4),Cp G_2、Cp G_4在三組樣本中的甲基化率分布差異無統(tǒng)計(jì)學(xué)意義(p0.05)。Cp G_3在三組樣本甲基化率分布差異具有統(tǒng)計(jì)學(xué)意義(p0.05)。維吾爾族飼鴿者肺患病組、維吾爾族飼養(yǎng)鴿子未發(fā)病組Cp G_3甲基化率高于維吾爾族未飼養(yǎng)鴿子健康體檢組,差異具有統(tǒng)計(jì)學(xué)意義(P0.017)。但維吾爾族飼鴿者肺患病組與維吾爾族飼養(yǎng)鴿子未發(fā)病組Cp G_3甲基化率差異無統(tǒng)計(jì)學(xué)意義(P0.017)。結(jié)論:接觸鴿子會(huì)影響HLA-DQA1基因甲基化率。但HLA-DQA1基因甲基化對(duì)維吾爾族飼鴿者肺的發(fā)病有無影響尚需進(jìn)一步研究。
[Abstract]:Objective: to detect the degree of methylation of HLA-DQA1 gene in the peripheral blood of the Uygur pigeons and to explore its significance. Methods: 20 cases of Uygur pigeon lung patients were selected as the case group, 20 cases of Uygur people who were not infected with dove were negative control group, and 20 Uygur people who didn't keep dove health check-up as normal control group. Collect data and extract peripheral blood samples. Routine extraction of DNA, bisulfite transformation, PCR amplification, in vitro transcription and RNase A specific enzyme digestion, and matrix assisted laser desorption ionization time of flight mass spectrometry technology were used to detect the methylation status of HLA-DQA1 gene. Then SPSS17.0 software was used for statistical analysis. The difference of HLA-DQA1 gene methylation rate between the three groups was compared. Then the difference of HLA-DQA1 methylation rate between the three groups was compared between 22 groups. Results: the total number of Cp G loci detected in HLA-DQA1 fragments was 4 (Cp G_1, Cp G_2, Cp G_3, Cp G_4). The number of 3 G_3 spots was detected in the 3 groups. There was no significant difference in the methylation rate distribution between the two groups. The difference of the methylation rate of Cp G_3 in the three groups was statistically significant (P0.05). The Cp G_3 methylation rate of Uygur pigeon lung disease group and Uygur rearing dove group was higher than that of Uygur non dove health examination group, the difference was statistically significant (P0.017). However, there was no significant difference in the Cp G_3 methylation rate between the Uygur pigeon group and the Uygur dove uninfected group (P0.017). Conclusion: exposure to dove can affect the methylation rate of HLA-DQA1 gene. However, the effect of HLA-DQA1 gene methylation on the lung of the Uygur pigeon is still needed to be further studied.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R563

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