【摘要】：目的探討妊娠期恐傷刺激對(duì)其80日齡仔鼠情志和海馬區(qū)去甲腎上腺素(NE)、腎上腺素(EP)的影響,并探討兩者的相關(guān)性。方法 2015年1月—2016年3月,選取80日齡SPF級(jí)Wistar大鼠59只(雌鼠34只,雄鼠25只),采用Morris水迷宮實(shí)驗(yàn)評(píng)價(jià)其認(rèn)知水平。選取認(rèn)知基線水平一致的30只雌鼠按照隨機(jī)數(shù)字表法分為模型組和對(duì)照組,各15只,另選取認(rèn)知基線水平一致且體格較強(qiáng)壯的15只雄鼠作為交配鼠。剩余雄鼠均作電擊鼠用,剩余雌鼠備用代替不孕鼠。運(yùn)用旁觀電擊法建立妊娠期恐傷大鼠動(dòng)物模型。仔鼠延續(xù)母鼠分組,至80日齡時(shí)采用隨機(jī)數(shù)字表法選取15只進(jìn)行實(shí)驗(yàn)。采用曠場(chǎng)實(shí)驗(yàn)、糖水偏好實(shí)驗(yàn)、懸尾實(shí)驗(yàn)評(píng)價(jià)仔鼠情志;通過(guò)腦立體定位儀在右側(cè)海馬區(qū)采集腦透析液,應(yīng)用高效液相色譜-電子俘獲檢測(cè)(HPLC-ECD)法檢測(cè)NE和EP水平。結(jié)果模型組曠場(chǎng)實(shí)驗(yàn)水平得分、垂直得分,糖水偏好實(shí)驗(yàn)糖水消耗量、糖水偏好值均低于對(duì)照組,懸尾實(shí)驗(yàn)靜止不動(dòng)時(shí)間長(zhǎng)于對(duì)照組(P0.05)。模型組各灌流時(shí)間點(diǎn)海馬組織NE、EP水平均低于對(duì)照組(P0.05)。仔鼠海馬組織NE水平與曠場(chǎng)實(shí)驗(yàn)水平得分、垂直得分以及糖水偏好實(shí)驗(yàn)糖水偏好值呈正相關(guān),與懸尾實(shí)驗(yàn)靜止不動(dòng)時(shí)間呈負(fù)相關(guān)(P0.05)。仔鼠海馬組織EP水平與曠場(chǎng)實(shí)驗(yàn)水平得分以及糖水偏好實(shí)驗(yàn)糖水偏好值呈正相關(guān),與懸尾實(shí)驗(yàn)靜止不動(dòng)時(shí)間呈負(fù)相關(guān)(P0.05)。結(jié)論恐傷孕鼠可改變其80日齡仔鼠的情志及海馬區(qū)NE和EP的水平,其情志改變與海馬區(qū)NE和EP的水平密切相關(guān)。
[Abstract]:Objective to investigate the effect of fear injury stimulation during pregnancy on norepinephrine (NE), epinephrine (EP) in 80-day-old mice and to explore the correlation between them. Methods from January 2015 to March 2016, 59 80-day-old SPF Wistar rats (34 female and 25 male) were selected and their cognitive level was evaluated by Morris water maze test. Thirty female rats with the same cognitive baseline level were randomly divided into model group (n = 15) and control group (n = 15). Another 15 male rats with the same cognitive baseline level and strong physique were selected as mating mice. The remaining male mice were used as electric shock mice, and the remaining female mice were set aside to replace infertile mice. The animal model of fear injury during pregnancy was established by bystander electric shock. The offspring were divided into groups and 15 mice were selected by random digital table at the age of 80 days. Open field test, sugar water preference test and tail suspension test were used to evaluate the emotion of neonatal rats. Brain dialysis fluid was collected in the right hippocampus by brain stereotactic apparatus, and the levels of NE and EP were detected by high performance liquid chromatography-electron capture detection (HPLC-ECD). Results the horizontal score, vertical score, sugar water preference test sugar water consumption and sugar water preference value of the model group were lower than those of the control group, and the rest time of the tail suspension test was longer than that of the control group (P 0.05). The level of NE,EP in hippocampus of the model group was lower than that of the control group at each perfusion time point (P 0.05). The level of NE in hippocampus of neonatal rats was positively correlated with the horizontal score of open field test, vertical score and sugar water preference test, and negatively correlated with the rest time of tail suspension test (P 0.05). The level of EP in hippocampus was positively correlated with the score of open field test and the value of sugar water preference in sugar water preference test, and negatively correlated with the rest time of tail suspension test (P 0.05). Conclusion the emotion of 80-day-old pregnant rats can be changed, and the levels of NE and EP in hippocampus can be changed. The emotional changes are closely related to the levels of NE and EP in hippocampus.
【作者單位】： 河南中醫(yī)藥大學(xué)中西醫(yī)結(jié)合基礎(chǔ)學(xué)科;
【基金】：國(guó)家自然科學(xué)基金面上項(xiàng)目(81373790)
【分類號(hào)】：R714

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