【摘要】：目的:探討抗神經(jīng)生長因子抗體(anti-NGF)對改善子宮腺肌病(AM)小鼠宮腔內(nèi)環(huán)境的作用。方法:將15只雌性ICR小鼠隨機分為AM治療組(A組)、AM溶劑對照組(B組)及正常對照組(C組),A、B組使用口服他莫昔芬法建立AM小鼠模型。A組小鼠腹腔注射anti-NGF,B組以等體積的生理鹽水替代,C組不作任何處理。3組小鼠于動情前期處死取雙子宮稱量后檢測緩激肽受體1(BKR-1)、神經(jīng)激肽1受體(NK-1R)、同源框基因轉(zhuǎn)錄因子A10(HOXA10)及芳香化酶(aromatase)基因與蛋白的表達。結(jié)果:A組子宮相對重量同B組,且均明顯高于C組(P0.05);A組BKR-1基因及蛋白和NK-1R基因水平較B組明顯降低(P0.05),與C組比較無統(tǒng)計學(xué)差異(P0.05);A組HOXA10基因及蛋白水平較B組明顯增高(P0.05),與C組比較無統(tǒng)計學(xué)差異(P0.05);A組芳香化酶基因及蛋白水平較B組減低(P0.05),但仍高于C組(P0.05)。結(jié)論:anti-NGF可顯著緩解AM模型小鼠子宮炎癥,改善內(nèi)膜容受性、抑制芳香化酶表達,但短期使用不能抑制病灶增殖。
[Abstract]:Aim: to investigate the effect of anti-nerve growth factor antibody (anti-NGF) on the improvement of uterine cavity environment in (AM) mice with adenomyosis. Methods: fifteen female ICR mice were randomly divided into AM treatment group (group A,), AM solvent control group, group B) and normal control group (group C,), A,). The AM mouse model was established by oral tamoxifen method in group B. the mice in group A were intraperitoneally injected with anti-NGF,B group instead of saline of the same volume. No treatment was performed in group C. the mice in group 3 were killed before estrus and weighing in the uterus. Bradykinin receptor 1 (BKR-1) and neurokinin 1 receptor (NK-1R) were detected. Expression of homeobox gene transcription factor A10 (HOXA10) and aromatase (aromatase) gene and protein. Results: the relative weight of uterus in group A was the same as that in group B, and it was significantly higher than that in group C (P 0.05). The levels of BKR-1 gene and protein and NK-1R gene in group); A were significantly lower than those in group B (P 0.05), but there was no significant difference between group A and group C (P 0.05). The level of HOXA10 gene and protein in group A was significantly higher than that in group B (P 0.05), but there was no significant difference between group A and group C (P 0.05). The level of aromatase gene and protein in group); A was lower than that in group B (P 0.05), but it was still higher than that in group C (P 0.05). Conclusion: anti-NGF can significantly alleviate uterine inflammation, improve intimal receptivity and inhibit aromatase expression in AM model mice, but short-term use can not inhibit the proliferation of lesions.
【作者單位】： 上海交通大學(xué)醫(yī)學(xué)院附屬瑞金醫(yī)院生殖醫(yī)學(xué)中心;
【基金】：2011年上海高校優(yōu)秀青年教師培養(yǎng)資助項目
【分類號】：R711.71

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