子癇前期相關(guān)miR-18a對人正常滋養(yǎng)細(xì)胞浸潤功能影響的研究
[Abstract]:Objective to study the effect of preeclampsia associated microRNA-18a (micro RNA-18a,mi R-18a) on the infiltration of human normal trophoblast (HTR8). To investigate the relationship between mi R-18a and preeclampsia and its role in the pathogenesis of preeclampsia. Methods mi R-18a precursor molecules were transfected into HTR8 cells and divided into three groups: pre-mi RNA negative control group, (NC), mi R-18a overexpression group and mi R-18a inhibition group; Reverse transcription PCR was used to detect the expression level of mi R-18a m RNA in each group, and invasive chamber experiment (Transwell) was used to detect the change of cell infiltration ability after transfection. Results compared with NC group (0.407 鹵0.019), the inhibitory effect of mi R-18a overexpression group was significant (0.884 鹵0.061 鹵0.161 鹵0.014), the difference was statistically significant (P0.05). Compared with NC group (66.67 鹵3.05), inhibiting the expression of mi R-18a significantly decreased the infiltrating ability of cells (29.631 鹵3.112 P 0.05). Conclusion mi R-18a can affect the infiltration function of human normal trophoblast to some extent and may be related to the pathogenesis of preeclampsia.
【作者單位】: 西安醫(yī)學(xué)院第一附屬醫(yī)院婦科;
【分類號】:R714.244
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