【摘要】：目的探討宮頸脫落細胞HPVL1蛋白、人乳頭瘤病毒(HPV)DNA載量對宮頸病變的預測作用。方法選取宮頸病變患者362例,宮頸刷采集宮頸脫落細胞,采用免疫化學方法檢測HPVL1蛋白,HC2技術(shù)檢測HPV DNA載量,根據(jù)宮頸組織病理學結(jié)果將患者分為慢性宮頸炎(190例)、宮頸上皮內(nèi)瘤變Ⅰ期(CINⅠ,45例)、宮頸上皮內(nèi)瘤變Ⅱ期(CINⅡ,43例)、宮頸上皮內(nèi)瘤變Ⅲ期(CINⅢ,46例)和宮頸癌(38例);根據(jù)宮頸細胞學檢查結(jié)果將脫落細胞分為未見異常細胞(NILM,122例),未明確性非典型鱗狀上皮細胞(ASCUS,75例),可疑非典型鱗狀上皮病變細胞(ASCH,52例),低度鱗狀上皮內(nèi)病變(LSIL,61例)和高度鱗狀上皮內(nèi)病變(HSIL,52例),比較不同宮頸病變HPVL1蛋白、HPV DNA載量的差異。結(jié)果 130例宮頸脫落細胞HPVL1蛋白陽性,陽性率為35.91%(130/362),其中HPVL1蛋白陽性率由大至小分別為:慢性宮頸炎CINⅠ期CINⅡ期CINⅢ期宮頸癌,NILMASCUSASCHLSILHSIL,不同宮頸病變組織病理學或細胞學類型比較差異具有統(tǒng)計學意義(P0.05),272例HPV DNA陽性,陽性率為75.14%,低度HPV DNA載量以慢性宮頸炎或NILM最多,高度HPV DNA載量以宮頸癌或HSIL最多,明顯高于其他宮頸組織病理學或細胞學類型(P0.05)。結(jié)論 HPVL1蛋白在宮頸脫落細胞的表達隨著宮頸病變程度加重而下調(diào),HPV DNA載量隨宮頸病變程度加重而上調(diào),二者檢測在宮頸病變程度的預測中具有重要的價值。
[Abstract]:Objective to investigate the predictive effect of HPVL1 protein and (HPV) DNA load of human papillomavirus (HPV) on cervical lesions. Methods Cervical exfoliated cells were collected from 362 patients with cervical lesions, HPVL1 protein was detected by immunocytochemical method and HPV DNA load was detected by HC2 technique. The patients were divided into chronic cervicitis (190 cases) according to the results of cervical histopathology. Cervical intraepithelial neoplasia stage 鈪，

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