【摘要】：目的研究卵巢癌特異性結(jié)合肽(ovarian cancer specific targeting peptide,OSTP)與順鉑(cis-dichlorodiamineplatinum,DDP)偶聯(lián)物(OSTP-DDP)對(duì)卵巢癌A2780細(xì)胞的靶向抑制作用。方法化學(xué)合成OSTP-DDP,體外培養(yǎng)卵巢癌A2780細(xì)胞,采用CCK-8法分別檢測(cè)OSTP-DDP和DDP對(duì)卵巢癌A2780細(xì)胞的生長(zhǎng)抑制作用;通過Annexin V-FITC凋亡試劑盒分別檢測(cè)OSTP-DDP和DDP對(duì)卵巢癌A2780細(xì)胞的周期和凋亡作用。結(jié)果通過質(zhì)譜分析和高效液相色譜(HPLC)分析,提示成功合成OSTP-DDP。CCK-8法檢測(cè)顯示,OSTP-DDP與DDP分別以不同濃度(10、20、40、80、160、320μmol·L~(-1))處理卵巢癌A2780細(xì)胞24、48、72 h后,均可對(duì)該細(xì)胞的生長(zhǎng)起到抑制作用,且呈時(shí)間、濃度依賴性。且OSTP-DDP的作用強(qiáng)于DDP(P0.05),提示OSTP-DDP具有靶向抑制細(xì)胞生長(zhǎng)作用。流式細(xì)胞術(shù)檢測(cè)結(jié)果顯示,經(jīng)OSTP-DDP和DDP處理后,細(xì)胞周期均被阻滯于G_1期,作用72 h后,OSTP-DDP對(duì)卵巢癌A2780細(xì)胞的周期抑制作用強(qiáng)于DDP,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。作用24、48、72 h后,OSTP-DDP對(duì)卵巢癌A2780細(xì)胞凋亡的作用強(qiáng)于DDP(P0.01)差異具有統(tǒng)計(jì)學(xué)意義,提示OSTP-DDP具有較強(qiáng)的靶向誘導(dǎo)細(xì)胞凋亡作用。結(jié)論 OSTP-DDP對(duì)卵巢癌A2780細(xì)胞的生長(zhǎng)具有靶向抑制作用,OSTP作為化療藥物靶向載體治療卵巢癌有良好的應(yīng)用前景。
[Abstract]:Objective to investigate the targeting inhibition of ovarian cancer A2780 cells by cisplatin (cis-dichlorodiamineplatinum,DDP) conjugate (OSTP-DDP) and ovarian cancer specific binding peptide (ovarian cancer specific targeting peptide,OSTP). Methods Ovarian cancer A2780 cells were cultured by chemically synthesized OSTP-DDP, and the growth inhibitory effects of OSTP-DDP and DDP on A2780 cells were detected by CCK-8 assay. The effects of OSTP-DDP and DDP on cell cycle and apoptosis of ovarian cancer A2780 cells were detected by Annexin V-FITC apoptosis kit. Results the results of mass spectrometry and high performance liquid chromatography (HPLC) showed that the successful synthetic OSTP-DDP.CCK-8 assay showed that OSTP-DDP and DDP were treated with different concentration (10 ~ 20 ~ 40 ~ (80) 160320 渭 mol / L ~ (-1) for 72 h, respectively. The growth of the cells was inhibited in a time and concentration dependent manner. The effect of OSTP-DDP was stronger than that of DDP (P 0.05), suggesting that OSTP-DDP had a targeted inhibitory effect on cell growth. The results of flow cytometry showed that after OSTP-DDP and DDP treatment, the cell cycle was blocked in G1 phase. After 72 h of treatment, the inhibitory effect of OSTP-DDP on A2780 cell cycle of ovarian cancer was stronger than that of DDP,. The difference was statistically significant (P0.05). The effect of OSTP-DDP on apoptosis of ovarian cancer A2780 cells was stronger than that of DDP (P0.01) after 72 h treatment, suggesting that OSTP-DDP had a strong targeting effect on inducing apoptosis of A2780 cells. Conclusion OSTP-DDP has a targeted inhibitory effect on the growth of ovarian cancer A2780 cells. OSTP as a targeted carrier of chemotherapeutic drugs has a good prospect in the treatment of ovarian cancer.
【作者單位】： 南華大學(xué)腫瘤研究所;南華大學(xué)附屬第一醫(yī)院臨床研究所;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(No 81472449,81101988)
【分類號(hào)】：R737.31
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本文編號(hào)：2347431