【摘要】：目的 通過檢測Livin和Beclin1蛋白在卵巢子宮內(nèi)膜異位癥（Ovarian endometriosis,OEMS）患者在位內(nèi)膜組織、異位內(nèi)膜組織及對照組正常子宮內(nèi)膜組織中的表達情況，分析Livin和Beclin1蛋白在各組織中表達的差異及二者之間有無相關(guān)性，進一步探討Livin和Beclin1蛋白在卵巢子宮內(nèi)膜異位癥發(fā)生機制中的作用。 研究方法 利用免疫組化染色法檢測Livin和Beclin1蛋白在對照組正常子宮內(nèi)膜和卵巢子宮內(nèi)膜異位癥患者在位內(nèi)膜、異位內(nèi)膜組織中的表達，分析上述因子在各組中表達的差異，，應(yīng)用SPSS13.0軟件對所得數(shù)據(jù)進行統(tǒng)計學(xué)處理，采用卡方檢驗及Fisher’s精確概率法及秩相關(guān)分析對其進行統(tǒng)計學(xué)分析，以α=0.05作為檢驗標(biāo)準(zhǔn)，P0.05具有統(tǒng)計學(xué)意義。 結(jié)果 1. Livin在實驗組陽性表達率均高于對照組正常子宮內(nèi)膜（P0.05），它在對照組正常子宮內(nèi)膜和實驗組在位內(nèi)膜、異位內(nèi)膜的陽性表達率分別為18.75%（9/48），60.00%（27/45），86.67%（39/45）。Livin在實驗組在位內(nèi)膜的陽性表達率高于對照組正常子宮內(nèi)膜（P0.05），在實驗組異位內(nèi)膜的陽性表達率高于在位內(nèi)膜及對照組正常子宮內(nèi)膜（P0.05）。Livin在實驗組在位內(nèi)膜、異位內(nèi)膜增生期與分泌期的表達均無差異（P0.05）。 2. Beclin1在實驗組陽性表達率均低于對照組正常子宮內(nèi)膜（P0.05），它在對照組正常子宮內(nèi)膜和實驗組在位內(nèi)膜、異位內(nèi)膜的陽性表達率分別為83.33%（40/48），48.89%（22/45），17.78%（8/45）。Beclin1在在位內(nèi)膜的陽性表達率低于正常子宮內(nèi)膜（P0.05），且Beclin1在異位內(nèi)膜的陽性表達率低于在位內(nèi)膜和正常子宮內(nèi)膜（P0.05）。Beclin1在對照組正常子宮內(nèi)膜組織中分泌期的表達高于增生期（P0.05），而在實驗組增生期與分泌期的表達差異無統(tǒng)計學(xué)意義（P0.05）。 3.實驗組在位內(nèi)膜和異位內(nèi)膜組織中Livin基因蛋白表達率Ⅲ-Ⅳ期均高于Ⅰ-Ⅱ期, P0.05，差異有統(tǒng)計學(xué)意義；Beclin1基因蛋白表達率Ⅲ-Ⅳ期均低于Ⅰ-Ⅱ期,P0.05，差異有統(tǒng)計學(xué)意義。 4. Livin和Beclin1在OEMS中的表達呈負相關(guān)。 結(jié)論 1. Livin蛋白在在位內(nèi)膜組、異位內(nèi)膜組中呈高表達，Beclin1蛋白呈低表達，且與OEMS臨床分期關(guān)系密切。 2.在OEMS中Livin和Beclin1的表達與月經(jīng)周期無關(guān)。 3. Livin和Beclin1在OEMS中的表達呈負相關(guān)，Livin/Beclin1作為一對負向調(diào)控蛋白在異位內(nèi)膜細胞的增殖、凋亡過程中發(fā)揮一定作用，共同促進OEMS的發(fā)生發(fā)展。
[Abstract]:Objective to detect the expression of Livin and Beclin1 protein in eutopic endometrium, ectopic endometrium and normal endometrium of patients with ovarian endometriosis (Ovarian endometriosis,OEMS). To analyze the difference of expression of Livin and Beclin1 in different tissues and the correlation between them, and to explore the role of Livin and Beclin1 in the pathogenesis of ovarian endometriosis. Methods Immunohistochemical staining was used to detect the expression of Livin and Beclin1 protein in eutopic endometrium and ectopic endometrium of normal endometrium and ovarian endometriosis in control group. The differences of the expression of the above factors in each group were analyzed. The data were statistically processed by SPSS13.0 software. The data were statistically analyzed by chi-square test, Fisher's accurate probability method and rank correlation analysis. With 偽 -0.05 as the test standard, P0.05 has statistical significance. Result 1. The positive expression rate of Livin in the experimental group was higher than that in the normal endometrium of the control group (P0.05), and it was expressed in the normal endometrium of the control group and the eutopic endometrium in the experimental group. The positive expression rates of ectopic endometrium were 18.75% (9 / 48) and 60.00% (27 / 45) respectively. The positive expression rate of Livin in eutopic endometrium in experimental group was higher than that in normal endometrium of control group (P0.05). The positive expression rate of ectopic endometrium in experimental group was higher than that in eutopic endometrium and control group (P0.05). (P0.05). Livin in eutopic endometrium of experimental group, There was no difference between ectopic endometrial hyperplasia and secretory phase (P0.05). The positive expression rate of Beclin1 in the experimental group was lower than that in the normal endometrium of the control group (P0.05), and it was expressed in the normal endometrium of the control group and the eutopic endometrium in the experimental group. The positive expression rates of Beclin1 in ectopic endometrium were 83.33% (40 / 48) 48.89% (22 / 45) and 17.78% (8 / 45). Beclin1 in eutopic endometrium was lower than that in normal endometrium (P0.05), and the positive expression rate of Beclin1 in ectopic endometrium was lower than that in eutopic endometrium and normal endometrium (P0.05). The expression of secretory phase in membrane tissue was higher than that in proliferative phase (P0.05), but there was no significant difference between proliferative phase and secretory phase in experimental group (P0.05). The expression rate of Livin gene protein in eutopic and ectopic endometrium in experimental group was higher than that in stage 鈪

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