【摘要】：目的研究尼古丁暴露孕鼠外周血代謝物改變特征,尋找可能反映胎鼠發(fā)育異常的早期特征性代謝物。方法將Wistar孕鼠16只分為空白對照組和尼古丁處理組,于孕9~20 d分別皮下注射生理鹽水和尼古丁[2 mg/(kg·d)],觀察孕鼠體重變化,并分析不同時間點(孕11、14、17、20 d)血漿1H NMR代謝譜改變。結(jié)果尼古丁處理后孕鼠體重增長減慢,孕20 d體重低于對照組(P0.01)。孕鼠血漿糖、脂和蛋白質(zhì)代謝相關(guān)的多種物質(zhì)發(fā)生改變,孕14 d為重要變化時間點。孕11~14 d血漿中檸檬酸、甘油、N-乙酰糖蛋白、纈氨酸、亮氨酸、色氨酸、賴氨酸、異亮氨酸、不飽和脂肪酸、低密度脂蛋白膽固醇、尿囊素濃度升高(P0.05),而丙酮酸、β-羥丁酸、肌醇濃度降低(P0.05)。結(jié)論 NMR代謝組學可作為孕鼠代謝改變檢測的技術(shù)手段,β-羥丁酸、丙酮酸、檸檬酸、不飽和脂肪酸、甘油、纈氨酸、亮氨酸可能作為母體外周血中反映胎兒發(fā)育異常的早期特征代謝物。
[Abstract]:Objective to study the changes of metabolites in peripheral blood of pregnant rats exposed to nicotine and to search for the early characteristic metabolites which may reflect the abnormal development of fetal mice. Methods Sixteen Wistar pregnant rats were divided into blank control group and nicotine treated group. Normal saline and nicotine [2 mg/ (kg d)] were injected subcutaneously on the 20th day of gestation. The changes of body weight were observed and the changes of 1H NMR metabolic spectrum in plasma at different time points (11141720 days) were analyzed. Results the weight of pregnant rats decreased after nicotine treatment, and the body weight of pregnant rats on day 20 was lower than that of control group (P0.01). The plasma glucose, lipid and protein metabolism related substances were changed in pregnant rats, and 14 days of gestation was an important time point of change. Plasma citric acid, glycerol N-acetylglycoprotein, valine, leucine, tryptophan, lysine, isoleucine, unsaturated fatty acids, low density lipoprotein cholesterol, allantoin were elevated (P0.05), while pyruvate, 尾 -hydroxybutyric acid, 尾 -hydroxybutyric acid, pyruvate, 尾 -hydroxybutyric acid, 尾 -hydroxybutyric acid The concentration of inositol decreased (P0.05). Conclusion NMR metabolomics can be used as a technique to detect metabolic changes in pregnant rats, such as 尾 -hydroxybutyric acid, pyruvate, citric acid, unsaturated fatty acid, glycerol, valine. Leucine may be an early characteristic metabolite in maternal peripheral blood reflecting fetal dysplasia.
【作者單位】： 武漢大學基礎(chǔ)醫(yī)學院藥理學系;
【基金】：國家自然科學基金(81430089);國家自然科學基金(81270950)
【分類號】：R714.5
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本文編號：2235618