【摘要】：目的探討輔助性T細(xì)胞17(Th17)、調(diào)節(jié)性T細(xì)胞(Treg)比例及其調(diào)控基因在卵巢子宮內(nèi)膜異位囊腫發(fā)生、發(fā)展中的作用。方法選擇卵巢子宮內(nèi)膜異位囊腫患者90例作為觀察組,因單純輸卵管原因?qū)е碌牟辉邪Y患者90例作為對(duì)照組。采用流式細(xì)胞術(shù)檢測(cè)兩組外周血Th17、Treg占CD4+T細(xì)胞比例,計(jì)算Th17/Treg。采用qRT-PCR法檢測(cè)觀察組異位內(nèi)膜、在位內(nèi)膜及對(duì)照組在位內(nèi)膜組織孤獨(dú)核受體γ(ROR-γ)mRNA、叉頭狀轉(zhuǎn)錄因子3(Fox P3)mRNA。采用ELISA法檢測(cè)兩組血清白細(xì)胞介素17(IL-17)、IL-22、IL-10、轉(zhuǎn)化生長(zhǎng)因子β(TGF-β)。結(jié)果觀察組外周血Th17占CD4+T細(xì)胞比例、Th17/Treg均高于對(duì)照組,Treg占CD4+T細(xì)胞比例低于對(duì)照組(P均0.05)。觀察組異位內(nèi)膜ROR-γmRNA相對(duì)表達(dá)量低于觀察組在位內(nèi)膜和對(duì)照組在位內(nèi)膜(P均0.05),觀察組在位內(nèi)膜ROR-γmRNA相對(duì)表達(dá)量低于對(duì)照組在位內(nèi)膜(P0.05);觀察組異位內(nèi)膜Fox P3 mRNA相對(duì)表達(dá)量高于觀察組在位內(nèi)膜和對(duì)照組在位內(nèi)膜(P均0.05),觀察組在位內(nèi)膜Fox P3 mRNA相對(duì)表達(dá)量高于對(duì)照組在位內(nèi)膜(P0.05)。觀察組血清IL-17、IL-22水平均高于對(duì)照組,IL-10、TGF-β水平均低于對(duì)照組(P均0.05)。結(jié)論卵巢子宮內(nèi)膜異位囊腫患者外周血Th17/Treg比例失衡;Th17/Treg比例失衡可能通過(guò)調(diào)節(jié)其下游轉(zhuǎn)錄因子及細(xì)胞因子表達(dá)而促進(jìn)卵巢子宮內(nèi)膜異位囊腫的發(fā)生、發(fā)展。
[Abstract]:Objective to investigate the role of helper T cell 17 (Th17), regulatory T cell (Treg) ratio and its regulatory gene in the development of ovarian endometriosis cysts. Methods 90 cases of ovarian endometriosis cyst were selected as observation group and 90 cases of infertility caused by simple fallopian tube as control group. The ratio of Th17,Treg to CD4 T cells in peripheral blood of two groups was detected by flow cytometry, and Th17/Treg. was calculated. Detection of mononuclear receptor 緯 (ROR- 緯) mRNA, forkhead transcription factor 3 (Fox P3) mRNA. in eutopic endometrium, eutopic endometrium and eutopic endometrium by qRT-PCR assay Serum interleukin-17 (IL-17) IL-22 and IL-10, transforming growth factor 尾 (TGF- 尾) were detected by ELISA. Results the ratio of Th17 to CD4 T cells in the observation group was significantly higher than that in the control group (P < 0. 05). The relative expression of ROR- 緯 mRNA in ectopic endometrium in observation group was lower than that in eutopic endometrium of observation group and in eutopic endometrium of control group (P 0.05), and the relative expression of ROR- 緯 mRNA in eutopic endometrium in observation group was lower than that in eutopic endometrium of control group (P0.05); The relative expression of Fox P3 mRNA in eutopic endometrium of observation group was higher than that in eutopic endometrium of control group (P 0.05), and the relative expression of P3 mRNA in eutopic endometrium in observation group was higher than that in eutopic endometrium of control group (P0.05). The level of serum IL-17,IL-22 in the observation group was higher than that in the control group (P < 0. 05). Conclusion the imbalance of Th17/Treg ratio in peripheral blood of patients with ovarian endometriosis and the imbalance of Th17 / Treg ratio may promote the occurrence and development of ovarian endometriosis cysts by regulating the expression of transcription factors and cytokines downstream.
【作者單位】： 青海紅十字醫(yī)院;
【分類號(hào)】：R711.71

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