【摘要】：目的:研究陜西省商洛地區(qū)婦女人乳頭瘤病毒(human papillomavirus,HPV)的感染狀況和亞型分布,為HPV分子流行病學(xué)和宮頸癌的防治提供依據(jù)。方法:選擇2012年6月至2014年7月在商洛中心醫(yī)院體檢、門(mén)診就診的937例患者的宮頸脫落細(xì)胞標(biāo)本,采用聚合酶鏈反應(yīng)(PCR)體外擴(kuò)增和脫氧核糖核酸(DNA)反向點(diǎn)雜交相結(jié)合的DNA芯片技術(shù)進(jìn)行HPV基因分型檢測(cè)。結(jié)果:HPV檢出率937例標(biāo)本中HPV感染566例,總感染率為60.41%(566/937),其中高危型感染病例總檢出率為51.44%(482/937);低危型感染總檢出率26.47%(248/937);感染年齡分布以61歲年齡段感染率最高,為88.89%,其次是≤20歲年齡段(67.86%),51~60歲年齡段(62.90%),41~50歲年齡段(60.29%),各個(gè)年齡組的感染率沒(méi)有統(tǒng)計(jì)學(xué)差異(P0.05)。23種HPV基因亞型中,高危感染中以16型最高,占26.58%(277/1042),其次是58型,占8.93%(93/1024),66型,占6.53%(68/1024),低危感染中以6型最高,占12.38%(129/1024),其次是43型,占4.32%(45/1024),42型,占4.22%(44/1024)。單一型別占總感染例數(shù)的50.53%(286/566),多重感染以二重感染最多,占總感染例數(shù)的12.92%(158/566)。結(jié)論:陜西省商洛地區(qū)HPV感染率高,主要亞型為HPV16、HPV58、HPV66,提示HPV亞型的分析對(duì)疫苗的研發(fā)和宮頸癌的篩查及防治有指導(dǎo)意義。
[Abstract]:Objective: to study the infection status and subtype distribution of human papillomavirus in Shangluo area, Shaanxi Province, and to provide evidence for the molecular epidemiology and prevention and treatment of cervical cancer. Methods: from June 2012 to July 2014, 937 specimens of cervical exfoliated cells were collected from 937 patients who underwent physical examination in Shangluo Central Hospital. HPV genotyping was detected by polymerase chain reaction (PCR) amplification in vitro and DNA reverse dot hybridization. Results among 937 specimens, 566 cases were infected with human papillomavirus (HPV), the total infection rate was 60.41% (566 / 937), among which 51.44% (482 / 937) were high risk type, 26.47% (248 / 937) were low risk type, and the highest was 61 years old. It was 88.89, followed by 鈮，

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