本文選題：缺誘導因子2 + 血管內皮生長因子��； 參考：《蘇州大學》2014年碩士論文

【摘要】：第一部分EPAS1、VEGF在子宮內膜癌中的表達情況 目的研究人子宮內膜癌及正常子宮內膜組織中缺氧誘導因子2(EPAS1/HIF-2α)、血管內皮生長因子(VEGF) mRNA及蛋白的表達及差異情況。 方法應用逆轉錄聚合酶鏈反應(RT-PCR)、免疫印跡(western blotting)法檢測46例子宮內膜癌及正常子宮內膜組織中EPAS1、VEGF mRNA和蛋白的表達，分析其間表達的差異。 結果EPAS1、VEGF在子宮內膜癌中的表達明顯高于正常子宮內膜組織, EPAS1mRNA和蛋白水平均增高(t=4.5871,p=0.0048;t=19.4877,p=0.0001)，VEGF在mRNA和蛋白水平均增高(t=7.0495,p=0.0001;t=98.4145,p=0.0001)。 結論子宮內膜癌組織中EPAS1和VEGF呈過量表達,且EPAS1的表達與VEGF呈顯著正相關。 第二部分EPAS1、VEGF在子宮內膜癌中表達的臨床意義 目的研究子宮內膜癌組織中EPAS1、VEGF和微血管密度（MVD）的表達與臨床病理特征之間的關系。 方法應用免疫組化SP法檢測46例子宮內膜癌及正常子宮內膜組織中EPAS1、VEGF蛋白的表達和分布,同時檢測MVD作為判斷血管生成的指標，分析其間的相關性以及與腫瘤臨床病理特征之間的關系。 結果EPAS1、VEGF和MVD在子宮內膜癌組織中的表達明顯高于正常子宮內膜組織,三者之間的表達具有顯著相關性(EPAS1和VEGF之間, r=0.7358,P=0.0041;VEGF和MVD之間,r=0.8871,P=0.0001; EPAS1和MVD間, r=0.7360,P=0.0003),EPAS1和VEGF的陽性表達與子宮內膜癌的FIGO分期、浸潤深度有關。 結論子宮內膜癌組織中EPAS1和VEGF呈過量表達,且EPAS1的表達與VEGF及MVD呈顯著正相關。EPAS1可通過上調VEGF表達來促進子宮內膜癌血管生成從而在子宮內膜癌的發(fā)生發(fā)展中起重要作用。
[Abstract]:Part one: expression of EPAS1 + VEGF in endometrial carcinoma objective to study the expression of hypoxia inducible factor 2 (EPAS1 / HIF-2 偽) and vascular endothelial growth factor (VEGF) mRNA and protein in human endometrial carcinoma and normal endometrial tissues. Methods reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot (western blotting) were used to detect the expression of EPAS1 (western blotting) mRNA and protein in 46 cases of endometrial carcinoma and normal endometrium. Results the expression of VEGF in endometrial carcinoma was significantly higher than that in normal endometrial tissue. The mRNA and protein levels of EPAS1 in endometrial carcinoma were significantly higher than those in normal endometrial tissue (t = 4.5871). The expression of VEGF in endometrial carcinoma was significantly higher than that in normal endometrial tissue (t = 4.5871). The mRNA and protein levels of VEGF in endometrial carcinoma were both increased (t = 7.0495p0.0001p0.0001t98.4145p0.0001). Conclusion the expression of EPAS1 and VEGF in endometrial carcinoma is overexpression, and the expression of EPAS1 is positively correlated with VEGF. The clinical significance of the expression of EPAS1 / VEGF in endometrial carcinoma objective to study the relationship between the expression of EPAS1 / VEGF and microvessel density (MVD) and clinicopathological features in endometrial carcinoma. Methods Immunohistochemical SP method was used to detect the expression and distribution of EPAS1 + VEGF protein in 46 cases of endometrial carcinoma and normal endometrium, and MVD was used as an index to judge angiogenesis. The relationship between the correlation and clinicopathological features of tumor was analyzed. Results the expression of VEGF and MVD in endometrial carcinoma was significantly higher than that in normal endometrial tissue. There was a significant correlation between the expression of EPAS1 and VEGF, the positive expression of EPAS1 and VEGF, and the positive expression of EPAS1 and VEGF in endometrial carcinoma were correlated with the Figo stage and the depth of invasion of endometrial carcinoma, and the positive expression of EPAS1 and VEGF was related to the stage and depth of invasion of endometrial carcinoma, and the positive expression of EPAS1 and VEGF was related to the stage and depth of invasion of endometrial carcinoma. Conclusion the expression of EPAS1 and VEGF in endometrial carcinoma is overexpression, and the expression of EPAS1 is positively correlated with VEGF and MVD. EPAS1 can promote angiogenesis of endometrial carcinoma by up-regulating VEGF expression, which may play an important role in the occurrence and development of endometrial carcinoma.
【學位授予單位】：蘇州大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R737.33

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