本文選題：基于NMR的代謝組學 + 妊娠期糖尿病(GDM)�。� 參考：《廈門大學》2014年碩士論文

【摘要】：代謝組學是20世紀90年代末期發(fā)展起來的一門新興學科,它借助高通量、高靈敏度的現(xiàn)代分析技術(shù),分析生物體中內(nèi)源性代謝物的整體組成,并通過跟蹤代謝物復雜、動態(tài)的變化,辨識和解析被研究對象的生理病理狀態(tài)。 妊娠期糖尿病(gestational diabetes mellitus, GDM)是糖尿病的一種獨立類型,是在妊娠期特發(fā)的代謝性疾病。本文將基于核磁共振(NMR)的代謝組學方法應(yīng)用于GDM的研究中,分析GDM志愿者和健康孕婦志愿者的血清和尿液中小分子代謝物的差異,探索GDM的生物標記物和受損代謝通路,為GDM的病理機制研究提供新的科學依據(jù)。本文的主要內(nèi)容歸納如下： 首先,詳細綜述了代謝組學方法在GDM研究中的應(yīng)用,總結(jié)并列舉了代謝組學在GDM研究中的優(yōu)勢。 其次,應(yīng)用1H NMR分析了GDM患者和健康孕婦血清和尿液的代謝輪廓,并結(jié)合多元統(tǒng)計分析發(fā)現(xiàn)：GDM患者的血清中葡萄糖、氧化三甲胺、琥珀酸和甘氨酸等代謝物含量明顯升高；尿液中甘氨酸、二甲基甘氨酸、尿黑酸、N-甲基-煙酰胺(N-methylnicotinamide, NMND)等含量升高,而丙二酸、乙醇胺、苯乙酰谷氨酰胺(phenylacetylglutamine, PAG)、馬尿酸、抗壞血酸等含量降低等現(xiàn)象。結(jié)果說明GDM不僅影響了機體的糖類、脂類和氨基酸的代謝,也導致了能量代謝以及肝腸循環(huán)等其他代謝系統(tǒng)的紊亂。尤其到了妊娠期糖尿病晚期,隨著胰島素抵抗作用增強,物質(zhì)和能量的代謝也逐步增強,導致機體代謝進一步失衡,加重肝腎的負擔,從而引起肝腎功能失調(diào)。 基于NMR的代謝組學分析方法為探討GDM的病理機制提供一個很好的技術(shù)平臺,并將在GDM的早期診斷和個體化治療等方面的研究具有廣闊的應(yīng)用前景。
[Abstract]:Metabonomics is a new discipline developed in the late 1990s. It uses modern analytical techniques with high throughput and high sensitivity to analyze the whole composition of endogenous metabolites in organisms, and it is complex by tracking metabolites. Dynamic changes, identification and analysis of the physiological and pathological state of the subjects studied. Gestational diabetes mellitus (gestational diabetes mellitus,) is an independent type of diabetes mellitus. In this paper, the nuclear magnetic resonance (NMR) based metabolomics method was applied to the study of GDM. The differences of small molecular metabolites in serum and urine between GDM volunteers and healthy pregnant women were analyzed, and the biomarkers and impaired metabolic pathways of GDM were explored. To provide a new scientific basis for the study of pathological mechanism of GDM. The main contents of this paper are summarized as follows: firstly, the application of metabonomics in GDM research is reviewed in detail, and the advantages of metabonomics in GDM research are summarized and enumerated. Secondly, the metabolic profiles of serum and urine of GDM patients and healthy pregnant women were analyzed by 1H NMR. The results of multivariate statistical analysis showed that the contents of glucose, trimethylamine oxide, succinic acid and glycine in serum of patients with GDM increased significantly. The contents of glycine, dimethyl glycine, N-methylated nicotinamide (NMND) in urine increased, while malonic acid, ethanolamine, phenylacetylglutamine (PAG), hippuric acid and ascorbic acid decreased. The results showed that GDM not only affected the metabolism of carbohydrate, lipids and amino acids, but also caused the disorder of energy metabolism and other metabolic systems such as liver and intestine circulation. Especially in the late gestational diabetes mellitus, with the increase of insulin resistance, the metabolism of substance and energy is gradually enhanced, which leads to the further imbalance of body metabolism, aggravating the burden of liver and kidney, thus causing the disorder of liver and kidney function. The method of metabolomics analysis based on NMR provides a good technical platform for discussing the pathological mechanism of GDM and will be widely used in the early diagnosis and individualized treatment of GDM.
【學位授予單位】：廈門大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R714.256;R445.2

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本文編號：2057528