本文選題：宮頸癌 + 人乳頭瘤病毒��； 參考：《青海大學(xué)》2014年碩士論文

【摘要】：宮頸癌（cervical cancer）是女性生殖系統(tǒng)常見的惡性腫瘤之一，是全球性公共衛(wèi)生問題。2008年全球新發(fā)宮頸癌病例約52.98萬，死亡病例25.51萬，其中85%新發(fā)病例在發(fā)展中國家。研究認(rèn)為，宮頸癌是由宮頸上皮內(nèi)瘤變（cervical intraepithelial neoplasias,CIN）逐步發(fā)展而來的，而宮頸癌的發(fā)生、發(fā)展又是多種危險(xiǎn)因素綜合作用的結(jié)果，其中通過性傳播使宮頸感染高危型人乳頭瘤病毒（high-risk human papillomavirus,HR-HPV）是宮頸癌及其癌前病變的高危型致病因素之一。HR-HPV感染時(shí)可以通過醫(yī)療干預(yù)來預(yù)防和治愈的，因此，早期發(fā)現(xiàn)HR-HPV感染并采取積極干預(yù)措施是防治宮頸癌及癌前病變的關(guān)鍵。 目的：利用實(shí)時(shí)熒光定量PCR法檢測(cè)高危型人乳頭瘤病毒DNA，結(jié)合病理診斷分析子宮頸病變與高危型人乳頭瘤病毒感染情況，為子宮頸癌與宮頸上皮內(nèi)瘤變的早期篩查提供一定的數(shù)據(jù)參考。 方法：以青海大學(xué)附屬醫(yī)院婦產(chǎn)科2011年6月—2012年10月63例患子宮疾病的婦女作為研究對(duì)象，采用實(shí)時(shí)熒光定量PCR法檢測(cè)63例子宮頸疾病患者宮頸分泌物中的高危型人乳頭瘤病毒DNA，對(duì)檢測(cè)結(jié)果進(jìn)行收集、整理，結(jié)合病理診斷分析子宮頸病變與高危型人乳頭瘤病毒感染情況。 結(jié)果：采用實(shí)時(shí)熒光定量PCR法檢測(cè)63例子宮頸分泌物中的HR-HPV DNA，結(jié)果顯示血漿HPV-16DNA檢出率為：宮頸炎11.1%、CINⅡ期20%、CINⅢ期20%、宮頸癌不同分期檢出率為：33.3%、58.3%、75%；血漿HPV-18陽性數(shù)結(jié)果分別為：宮頸炎16.7%、CINⅡ期16.6%、CINⅢ期10%、宮頸癌不同分期檢出率為：15%、16.6%、16.6%。統(tǒng)計(jì)學(xué)分析提示宮頸癌臨床分期與血漿病毒檢出率顯著相關(guān)(P0.05) 結(jié)論：實(shí)時(shí)熒光定量PCR法檢測(cè)血液中人乳頭瘤病毒DNA是比較靈敏的，，用這種方法可以在宮頸癌的篩查中起到一定的作用。子宮頸病變的發(fā)生、發(fā)展與HR-HPV感染有關(guān)，隨子宮頸病變的加重，HR-HPV感染陽性率明顯升高。
[Abstract]:Cervical cancer is one of the most common malignant tumors in the female reproductive system, which is a global public health problem. In 2008, there were about 529800 new cervical cancer cases and 255100 deaths in the world, of which 85% of the new cases were in developing countries. It is believed that cervical cancer is developed from cervical intraepithelial neoplasias cin, and the occurrence and development of cervical cancer is the result of multiple risk factors. High-risk human papillomavirusHR-HPVs through sexual transmission are one of the high risk factors for cervical cancer and its precancerous lesions. HR-HPV infection can be prevented and cured by medical intervention. Early detection of HR-HPV infection and active intervention are the key to prevent and treat cervical cancer and precancerous lesions. Objective: to detect the high risk human papillomavirus (HPVV) DNA by real-time fluorescence quantitative PCR, and to analyze the cervical lesions and human papillomavirus (HPVV) infection in combination with pathological diagnosis. To provide some data for early screening of cervical cancer and cervical intraepithelial neoplasia. Methods: from June 2011 to October 2012, 63 women with uterine diseases were enrolled in the study of gynecology and obstetrics in the affiliated Hospital of Qinghai University. High-risk human papillomavirus DNA in cervical secretions of 63 patients with cervical diseases was detected by real-time fluorescent quantitative PCR, and the results were collected and sorted out. Combined with pathological diagnosis, cervical lesions and high-risk human papillomavirus infection were analyzed. Results: HR-HPV DNA was detected by real-time fluorescence quantitative PCR in 63 cases of cervical secretion. The results showed that the detection rate of HPV-16 DNA in plasma was: cervicitis 11.1and cin 鈪

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