本文選題：膜聯(lián)蛋白A + HeLa��； 參考：《解剖學報》2016年04期

【摘要】：目的探討膜聯(lián)蛋白A5(ANXA5)低表達對人宮頸癌HeLa細胞遷移和侵襲的影響。方法將細胞分為干擾組、陰性對照組、轉染試劑對照組和空白對照組,采用脂質體轉染法將siRNA轉染干擾組HeLa細胞,在轉染72h后采用RT-PCR和Western blotting檢測各組ANXA5的表達以鑒定抑制效率;細胞劃痕實驗檢測各組HeLa細胞的遷移能力;Transwell實驗檢測各組HeLa細胞的侵襲能力;RT-PCR和Western blotting分別檢測ANXA5低表達對E-鈣黏蛋白(E-cadherin)和基質金屬蛋白酶(MMP)-9 mRNA及蛋白表達的影響。結果干擾組ANXA5蛋白及mRNA的表達均明顯低于陰性對照組(P0.05);其表達均被顯著抑制,干擾組細胞的遷移及侵襲能力比陰性對照組明顯增強(P0.05),E-cadherin mRNA及蛋白的表達顯著低于陰性對照組(P0.05);干擾組MMP-9mRNA及蛋白的表達均顯著高于陰性對照組(P0.05)。結論靶向ANXA5的siRNA可有效抑制ANXA5的表達,且ANXA5低表達可能通過影響E-cadherin和MMP-9的表達來促進HeLa細胞的遷移和侵襲能力。
[Abstract]:Objective to investigate the effect of low expression of A5 A5 on migration and invasion of human cervical carcinoma HeLa cells. Methods the cells were divided into three groups: interference group, negative control group, transfection reagent control group and blank control group. SiRNA was transfected into HeLa cells by liposome transfection. After 72 hours of transfection, RT-PCR and Western blotting were used to detect the expression of ANXA5 in each group to identify the inhibition efficiency. The migration ability of HeLa cells in each group was detected by cell scratch assay. The invasion ability of HeLa cells in each group was detected by Transwell assay. The effects of low expression of ANXA5 on the expression of E-cadherin (E-cadherin) and matrix metalloproteinase-9 (MMP-9) and protein were detected by RT-PCR and Western blotting, respectively. Results the expression of ANXA5 protein and mRNA in the interference group was significantly lower than that in the negative control group (P 0.05). The expression of E-cadherin mRNA and protein in the interference group was significantly lower than that in the negative control group, and the expression of MMP-9mRNA and protein in the interference group was significantly higher than that in the negative control group. Conclusion siRNA targeting ANXA5 can effectively inhibit the expression of ANXA5, and the low expression of ANXA5 may promote the migration and invasion of HeLa cells by affecting the expression of E-cadherin and MMP-9.
【作者單位】： 承德醫(yī)學院基礎醫(yī)學研究所;承德市266醫(yī)院感染科;
【基金】：河北省高校省級重點學科項目(201304) 河北省科技廳課題(10276142) 河北省教育廳項目(Z2014025)
【分類號】：R737.33

【相似文獻】

相關期刊論文 前1條

1 居小兵;沈百欣;徐子程;沈華;馬隆;蔣榮江;張煒;王心如;;鄰苯二甲酸二丁酯孕期暴露致仔鼠睪丸膜聯(lián)蛋白A5的表達差異[J];中國組織工程研究與臨床康復;2008年50期

，

本文編號：1837009