發(fā)布時間：2018-04-22 08:20

  本文選題：抗sca-抗體 + 堿性成纖維細(xì)胞生長因子�。� 參考：《第三軍醫(yī)大學(xué)學(xué)報》2017年22期

【摘要】：目的觀察負(fù)載抗sca-1抗體(anti-sca-1)和堿性成纖維細(xì)胞生長因子(basic fibroblast growth factor,b FGF)-脫細(xì)胞膀胱基質(zhì)(urinary bladder matrix,UBM)生物支架的生物學(xué)性能,探討其作為新型盆底修復(fù)材料的可能性。方法通過雙特異性化學(xué)交聯(lián)試劑4-(N-馬來酰亞胺甲基)環(huán)己烷-1-羧酸磺酸基琥珀酰亞胺酯鈉鹽(sulfosuccinimidyl 4-[N-maleimidomethyl]cyclohexane-1-carboxylate,sulfosmcc)將anti-sca-1和b FGF特異性結(jié)合到UBM上,得到anti-sca-1/b FGF-UBM生物支架。通過掃描電子顯微鏡觀察生物支架表面結(jié)構(gòu),CCK-8(cell counting kit-8)間接檢測體外富集sca-1陽性細(xì)胞情況,免疫熒光觀察支架誘導(dǎo)小鼠骨髓間充質(zhì)干細(xì)胞(mouse mesenchymal stem cells,m MSCs)分化成熟情況。結(jié)果掃描電子顯微鏡顯示anti-sca-1和b FGF能特異性的結(jié)合到UBM上。anti-sca-1和b FGF組熒光強(qiáng)度明顯高于對照組(P0.05)。anti-sca-1/b FGF-UBM生物支架富集的sca-1陽性細(xì)胞明顯較對照組多(P0.05)。免疫熒光觀察結(jié)果提示anti-sca-1/b FGF-UBM生物支架可以促進(jìn)m MSCs向平滑肌細(xì)胞分化。結(jié)論 anti-sca-1/b FGF-UBM生物支架顯示出了良好的生物性能,既能富集sca-1陽性細(xì)胞,又能進(jìn)一步促進(jìn)細(xì)胞分化成熟。
[Abstract]:Objective to investigate the biological properties of scaffolds loaded with anti-sca-1 (anti-sca-1) and basic fibroblast growth factor-basic fibroblast growth factor-acellular bladder matrix (bladder), and to explore the possibility of using the scaffold as a new pelvic floor repair material. Methods anti-sca-1 and b FGF were specifically conjugated to UBM by bispecific chemical crosslinking reagent 4-N- maleimide methyl) cyclohexane-1-carboxylic acid sulfonyl succinimidyl 4- [N-maleimidomethyl] cyclohexane-1-carboxylate sulfoxylsuccinyl 4- [N-maleimidomethyl] cyclohexane-1-carboxylatesulfosmcc. the anti-sca-1/b FGF-UBM scaffolds were obtained. The surface structure of CCK-8 cell counting kit-8 was observed by scanning electron microscope (SEM). The enrichment of sca-1 positive cells was detected indirectly in vitro. The differentiation and maturation of mesenchymal stem cells were observed by immunofluorescence in mice bone marrow mesenchymal stem cells (MSCs) induced by the scaffold. Results scanning electron microscopy showed that the fluorescence intensity of anti-sca-1 and b FGF binding to UBM. Anti-sca-1 and b FGF was significantly higher than that of control group (P0.05, anti-sca-1 / 1 / b FGF-UBM bioscaffold), and the number of sca-1 positive cells was significantly higher than that of control group. Immunofluorescence observation showed that anti-sca-1/b FGF-UBM scaffold could promote the differentiation of m MSCs into smooth muscle cells. Conclusion anti-sca-1/b FGF-UBM scaffolds have good biological properties, which can not only enrich sca-1 positive cells, but also further promote cell differentiation and maturation.
【作者單位】： 第三軍醫(yī)大學(xué)西南醫(yī)院婦產(chǎn)科;
【基金】：國家自然科學(xué)基金面上項目(81471443)~~
【分類號】：R318.08;R711.2
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本文編號：1786347