二氧化碳?xì)飧箤?duì)大鼠卵巢功能影響的研究
發(fā)布時(shí)間：2018-04-20 06:28
本文選題：二氧化碳?xì)飧?/strong> + 卵巢功能�。� 參考：《河北聯(lián)合大學(xué)》2014年碩士論文
【摘要】：目的探討不同時(shí)間、不同壓力下二氧化碳?xì)飧箤?duì)大鼠卵巢功能的影響。 方法將10周齡健康雌性SD大鼠250只觀察性周期后采用處于動(dòng)情間期大鼠隨即分為對(duì)照組、氣腹組及無(wú)氣腹組。氣腹組分別于15mmHg、20mmHg壓力下充入醫(yī)用CO2氣體1h、2h，并分別于氣腹結(jié)束后0h、6h、12h、24h取下腔靜脈血及雙側(cè)卵巢組織。無(wú)氣腹組僅進(jìn)行相關(guān)手術(shù)操作，腹腔內(nèi)不充入CO2氣體，取下腔靜脈血及雙側(cè)卵巢組織時(shí)間同氣腹組。對(duì)照組直接處死取下腔靜脈血及雙側(cè)卵巢組織。雙抗體夾心酶聯(lián)免疫（ELISA）法測(cè)定大鼠血清FSH、LH、E2、P含量。取雙側(cè)卵巢組織，黃嘌呤氧化酶法檢測(cè)超氧化物歧化酶（SOD）的活性、硫代巴比妥酸法卵巢丙二醛(MDA)，卵巢組織取做HE染色觀察卵巢內(nèi)細(xì)胞形態(tài)。 結(jié)果1大鼠血清內(nèi)雌二醇（E2）、孕酮（P）的變化：充氣1h后，氣腹結(jié)束后大鼠血清中含量較對(duì)照組明顯下降（p＜0.01）；氣腹結(jié)束6h后血清中含量較對(duì)照組下降，且較氣腹結(jié)束后0h含量下降；12h后血清中含量較氣腹結(jié)束后6h下降（p＜0.01），比較對(duì)照組、0h、6h下降（p＜0.01）；氣腹結(jié)束24h后較對(duì)照組、氣腹結(jié)束0h、6h、12h下降（p＜0.01）；充氣2h后變化同充氣1h，在相同時(shí)間點(diǎn)的變化較充氣1h下降（p＜0.01）。20mmHg壓力下變化同上，相同時(shí)間點(diǎn)變化較15mmHg更顯著（p＜0.05）；2大鼠血清FSH、LH變化相同，氣腹結(jié)束后大鼠血清中氣腹含量明顯高于對(duì)照組（p＜0.01）；氣腹結(jié)束6h后血清中含量較對(duì)照組升高，且較氣腹結(jié)束后0h含量升高；12h后血清中含量較氣腹結(jié)束后6h升高（p＜0.01），比較對(duì)照組、0h、6h升高（p＜0.01）；氣腹結(jié)束24h后較對(duì)照組、氣腹結(jié)束0h、6h、12h升高；氣腹結(jié)束24h后較對(duì)照組、氣腹結(jié)束0h、6h、12h升高（p＜0.01）；充氣2h后變化同充氣1h，在相同時(shí)間點(diǎn)的變化較充氣1h升高（p＜0.01）；20mmHg壓力下變化同上，相同時(shí)間點(diǎn)變化較15mmHg更顯著（p＜0.05）；3大鼠卵巢組織中SOD含量：氣腹結(jié)束后大鼠血清中含量較對(duì)照組明顯下降（p＜0.01）；氣腹結(jié)束6h后血清中含量較對(duì)照組下降，且較氣腹結(jié)束后0h含量下降；12h后血清中含量較氣腹結(jié)束后6h下降（p＜0.01），比較對(duì)照組、0h、6h下降（p＜0.01）；氣腹結(jié)束24h后較對(duì)照組、氣腹結(jié)束0h、6h、12h下降（p＜0.01）；氣腹結(jié)束24h后較對(duì)照組、氣腹結(jié)束0h、6h、12h下降（p＜0.01）；充氣2h后變化同充氣1h，在相同時(shí)間點(diǎn)的變化較充氣1h下降（p＜0.01），20mmHg壓力下變化同上，相同時(shí)間點(diǎn)變化較15mmHg更明顯（p＜0.05）；4大鼠卵巢組織中MDA含量：氣腹結(jié)束后大鼠血清中含量較對(duì)照組提高（p＜0.01）；氣腹結(jié)束6h后血清中含量較對(duì)照組升高，且較氣腹結(jié)束后0h含量升高；12h后血清中含量較氣腹結(jié)束后6h升高（p＜0.01），比較對(duì)照組、0h、6h升高（p＜0.01）；氣腹結(jié)束24h后較對(duì)照組、氣腹結(jié)束0h、6h、12h升高；于0h、6h、12h、24h均升高（p＜0.01）；充氣2h后變化同充氣1h，在相同時(shí)間點(diǎn)的變化較充氣1h升高（p＜0.01），20mmHg壓力下變化同上，相同時(shí)間點(diǎn)變化較15mmHg更明顯（p＜0.05）；5卵巢HE染色結(jié)果顯示：氣腹結(jié)束后0h可見(jiàn)間質(zhì)細(xì)胞輕度水腫，細(xì)胞體積增大，胞漿疏松；6h可見(jiàn)間質(zhì)細(xì)胞及部分顆粒細(xì)胞、卵泡膜細(xì)胞輕度水腫，細(xì)胞體積增大，胞漿染色變淺，血管擴(kuò)張，少量炎細(xì)胞浸潤(rùn)；12h可見(jiàn)間質(zhì)細(xì)胞及顆粒細(xì)胞、卵泡膜細(xì)胞水腫較前明顯，細(xì)胞體積較前增大，胞漿透明，呈氣球樣，細(xì)胞核增大，血管擴(kuò)張，，炎細(xì)胞浸潤(rùn)明顯；24h后改變更明顯。壓力越大，細(xì)胞水腫越明顯。 結(jié)論1大鼠血清中的E2、P在相同壓力下隨氣腹形成時(shí)間延長(zhǎng)及結(jié)束時(shí)間延長(zhǎng)而下降；不同壓力下隨氣腹形成時(shí)間延長(zhǎng)及結(jié)束時(shí)間延長(zhǎng)而下降，且壓力越大，下降越明顯；2大鼠血清中FSH、LH在相同壓力下隨氣腹形成時(shí)間延長(zhǎng)及結(jié)束時(shí)間延長(zhǎng)而升高；3卵巢組織中SOD含量在相同壓力下隨氣腹形成時(shí)間延長(zhǎng)及結(jié)束時(shí)間延長(zhǎng)而下降；不同壓力下隨氣腹形成時(shí)間延長(zhǎng)及結(jié)束時(shí)間延長(zhǎng)而下降，且壓力越大，下降越明顯；MDA的含量在相同壓力下隨氣腹形成時(shí)間延長(zhǎng)及結(jié)束時(shí)間延長(zhǎng)而升高；不同壓力下隨氣腹形成時(shí)間延長(zhǎng)及結(jié)束時(shí)間延長(zhǎng)而升高，且壓力越大，升高越明顯；4相同壓力下，氣腹形成時(shí)間越長(zhǎng)，卵巢組織內(nèi)間質(zhì)及顆粒細(xì)胞、卵泡膜細(xì)胞水腫明顯，隨氣腹結(jié)束時(shí)間延長(zhǎng)，變化越明顯；20mmHg時(shí)上述結(jié)構(gòu)變化較15mmHg壓力明顯；
[Abstract]:Objective to investigate the effects of carbon dioxide pneumoperitoneum on ovarian function in rats at different time and pressure.
Methods 250 healthy female SD rats of 10 weeks old were divided into control group, pneumoperitoneum group and no pneumoperitoneum group. The pneumoperitoneum group was filled with 1H and 2h of medical CO2 gas under the pressure of 15mmHg and 20mmHg, respectively. After the pneumoperitoneum, 0h, 6h, 12h, 24h took the inferior vena cava blood and bilateral ovarian tissue. No pneumoperitoneum group. The CO2 gas was not filled in the abdominal cavity, the blood of the inferior vena cava and the time of the bilateral ovarian tissue were taken with the pneumoperitoneum. The control group was directly executed to take the inferior vena cava blood and bilateral ovarian tissue. The content of FSH, LH, E2 and P in the serum of rats was measured by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). The bilateral ovarian tissue and xanthine oxidase method were measured. Superoxide dismutase (SOD) activity, thiobarbituric acid malondialdehyde (MDA), ovarian tissue HE staining were used to observe the morphology of ovarian cells.
Results the changes of serum estradiol (E2) and progesterone (P) in the 1 rats: after inflatable 1H, the serum content of rats decreased significantly after the end of pneumoperitoneum compared with the control group (P < 0.01). The content of serum in the pneumoperitoneum after 6h was lower than that of the control group, and the content of 0h decreased after the end of the pneumoperitoneum, and the serum content was lower than that of the pneumoperitoneum after the end of pneumoperitoneum (P < 0.01), and the comparison was compared with that after the end of pneumoperitoneum (P < 0.01). In the group, 0h, 6h decreased (P < 0.01); after the pneumoperitoneum ended 24h compared with the control group, the pneumoperitoneum ended with 0h, 6h, 12h (P < 0.01), the inflatable 2H was changed with the inflatable 1H, and the change in the same time point was higher than that of the inflatable 1H (P < 0.01), and the same time point was more significant than that of the inflatable 1H (< 0.05). 2 rat serum was similar, The content of pneumoperitoneum in the serum of rats after pneumoperitoneum was significantly higher than that of the control group (P < 0.01), and the content of serum in the 6h after the pneumoperitoneum was higher than that of the control group, and the content of 0h increased after the end of the pneumoperitoneum, and the content of the serum was higher than that of the control group after the end of the pneumoperitoneum (P < 0.01), compared with the control group, 0h, 6h increased (P < 0.01), and the pneumoperitoneum was compared with the control group after the pneumoperitoneum ended 24h. The abdominal end 0h, 6h, 12h increased, after the pneumoperitoneum ended 24h compared with the control group, 0h, 6h, 12h increased (P < 0.01) at the end of the pneumoperitoneum (P < 0.01); the change of inflatable 2H was higher than the inflatable 1H (P < 0.01) at the same time point (P < 0.05). The content of serum in rats after pneumoperitoneum was significantly lower than that of the control group (P < 0.01), the content of serum in the 6h after pneumoperitoneum was lower than that of the control group, and the 0h content decreased after the end of the pneumoperitoneum. After 12h, the content of serum was lower than 6H (P < 0.01) after the end of pneumoperitoneum (P < 0.01). Compared with the control group, 0h, 6h decreased (P < 0.01); after pneumoperitoneum ended 24h, the pneumoperitoneum was compared with the control group and pneumoperitoneum. The end of 0h, 6h, 12h decreased (P < 0.01); after the pneumoperitoneum ended 24h compared with the control group, the pneumoperitoneum ended 0h, 6h, 12h decreased (P < 0.01), the inflatable 2H changed with the inflatable 1H, and the change in the same time point was lower than that of the inflatable 1H (< 0.01). Content: after the end of pneumoperitoneum, the content of serum in rats increased (P < 0.01), and the content of serum in 6h after pneumoperitoneum was higher than that of control group, and the content of 0h increased after the end of pneumoperitoneum. After 12h, the content of serum was higher than that of 6h after pneumoperitoneum (P < 0.01). Compared with the control group, 0h, 6h increased (P < 0.01); after pneumoperitoneum ended 24h, the pneumoperitoneum was compared with the control group and pneumoperitoneum. At the end of 0h, 6h, and 12h, 0h, 6h, 12h, 24h increased (P < 0.01), and inflatable 1H with inflatable 2H (P < 0.01) at the same time point (P < 0.01). The same time point changes were more obvious than that of inflatable 1H (< 0.05). 5 ovary staining results showed that interstitial cells were mild after the end of the pneumoperitoneum. Edema, cell volume increased, cytoplasm loosely, 6h could be seen in interstitial cells and some granulosa cells, the follicle cells were edema, cell volume increased, cytoplasm staining became shallow, blood vessels dilated, small amount of inflammatory cells infiltrated, interstitial cells and granulosa cells were seen in 12h, the oedema of follicular cells was more obvious, cell volume was larger, cytoplasm transparent, and more transparent. Ballooning, enlarged nuclei, dilated blood vessels, inflammatory cell infiltration, and more obvious changes after 24h. The greater the pressure, the more obvious the cell edema.
Conclusion the E2 and P in the serum of 1 rats decreased with the prolongation of the time of pneumoperitoneum formation and the extension of the end time. Under the different pressure, the longer the formation time and the end time of the pneumoperitoneum, the greater the pressure, the more obvious. The FSH and LH in the serum of 2 rats were prolonged with the prolongation of the pneumoperitoneum and the end time under the same pressure. The content of SOD in the ovarian tissue decreased with the prolongation of the time of pneumoperitoneum and the extension of the end time under the same pressure. The greater the pressure of the pneumoperitoneum and the longer the end time, the decrease was more obvious under the pressure of the pneumoperitoneum. The content of MDA was prolonged with the time of the pneumoperitoneum and the end time under the same pressure. Under the same pressure, the longer the formation time of the pneumoperitoneum, the greater the formation time of the pneumoperitoneum, the edema of the ovarian tissue and granulosa cells, the follicle membrane cells in the ovarian tissue, the more obvious the change with the end of the pneumoperitoneum at the same time, and the above structure at 20mmHg at 20mmHg. The change is more obvious than that of 15mmHg.
【學(xué)位授予單位】：河北聯(lián)合大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R71
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