本文選題：上皮性卵巢癌 + 巨噬細胞　； 參考：《上海交通大學學報(醫(yī)學版)》2017年06期

【摘要】：目的·檢測TWEAK刺激后,巨噬細胞及其分泌的外泌體中miRNA-7的表達;探索TWEAK刺激后,巨噬細胞源性外泌體抑制上皮性卵巢癌細胞(HO8910-PM)侵襲和遷移的機制。方法·收集TWEAK刺激前后的巨噬細胞源性外泌體,并將其與HO8910-PM細胞共培養(yǎng),real-time PCR檢測巨噬細胞、巨噬細胞源性外泌體和共培養(yǎng)后HO8910-PM細胞中miRNA-7的表達;Western blotting檢測共培養(yǎng)后HO8910-PM細胞中EGFR/AKT/ERK1/2信號通路的表達;Antagomi R-7處理降低巨噬細胞中miRNA-7表達后,收集TWEAK刺激前后的外泌體并進行transwell侵襲和遷移實驗,觀察HO8910-PM細胞侵襲和遷移能力的變化。結(jié)果·TWEAK刺激后,巨噬細胞內(nèi)及其外泌體中miRNA-7的表達升高,并可上調(diào)HO8910-PM細胞中miRNA-7的表達,下調(diào)EGFR信號通路的表達。預先下調(diào)巨噬細胞中miRNA-7的表達后,巨噬細胞源性外泌體和共培養(yǎng)后HO8910-PM細胞中miRNA-7的表達降低,TWEAK刺激的巨噬細胞源性外泌體原先抑制HO8910-PM細胞轉(zhuǎn)移的作用得到恢復。結(jié)論·miRNA-7在TWEAK刺激巨噬細胞分泌的外泌體中發(fā)揮重要作用,可通過抑制上皮性卵巢癌細胞中EGFR信號通路抑制其侵襲和遷移。
[Abstract]:Objective to detect the expression of miRNA-7 in macrophages and exocrine secreted by TWEAK, and to explore the mechanism of macrophage derived exocrine inhibition of invasion and migration of epithelial ovarian cancer cell line HO8910-PMM stimulated by TWEAK.Methods macrophage derived exocrine bodies were collected before and after TWEAK stimulation and were co-cultured with HO8910-PM cells to detect macrophages by real-time PCR.Expression of miRNA-7 in macrophage derived exocrine and co-cultured HO8910-PM cells. Western blotting was used to detect the expression of EGFR/AKT/ERK1/2 signaling pathway in co-cultured HO8910-PM cells. Antagomi R-7 treatment decreased miRNA-7 expression in macrophages.The exocrine bodies were collected before and after TWEAK stimulation, and the invasion and migration of HO8910-PM cells were observed by transwell invasion and migration experiments.Results after TWEAK stimulation, the expression of miRNA-7 in macrophages and its exocrine increased, and the expression of miRNA-7 in HO8910-PM cells was up-regulated, and the expression of EGFR signal pathway was down-regulated.After down-regulating the expression of miRNA-7 in macrophages, the expression of miRNA-7 in macrophages derived from exocrine and co-cultured HO8910-PM cells decreased, and the inhibitory effect of TWEAK-stimulated macrophage derived exocrine on the metastasis of HO8910-PM cells was recovered.Conclusion miRNA-7 plays an important role in the secretion of macrophages stimulated by TWEAK and can inhibit the invasion and migration of epithelial ovarian cancer cells by inhibiting the EGFR signaling pathway.
【作者單位】： 上海交通大學醫(yī)學院附屬仁濟醫(yī)院婦產(chǎn)科上海市婦科腫瘤重點實驗室;
【基金】：國家自然科學基金(81272884) 上海市衛(wèi)生和計劃生育委員會科研項目(ZHYY-ZXYJHZX-2-06) 上海市教育委員會高峰高原學科建設(shè)計劃(20161412)~~
【分類號】：R737.31

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相關(guān)期刊論文 前2條

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本文編號：1742315