本文選題：核黃素　切入點：氯丙嗪　出處：《中國藥學(xué)雜志》2017年17期

【摘要】：目的體外研究核黃素(RIB)代謝途徑對順鉑(DDP)對卵巢癌HO8910細(xì)胞株黏附和遷移的作用。方法通過慢病毒包裝核黃素轉(zhuǎn)運體2(RFT2)shRNA干擾載體和RIB競爭性抑制劑氯丙嗪(CHL)干預(yù)RIB代謝途徑。HO8910卵巢癌細(xì)胞隨機分為空白對照組、shRNA對照組、RFT2 shRNA組、CHL(50μmol·L~(-1))組、DDP(20μmol·L~(-1))組、RFT2 shRNA+DDP組、CHL+DDP組和DDP+RIB(20μmol·L~(-1))組。各組細(xì)胞處理48 h后,細(xì)胞黏附實驗檢測細(xì)胞黏附能力;Transwell方法檢測細(xì)胞遷徙能力;Western blot檢測VEGF、MMP9和MMP2的表達(dá);激光共聚焦檢測TNF-α和NF-κB/p65的表達(dá)。結(jié)果與單獨給藥組對比,RFT2 shRNA或CHL聯(lián)合DDP能夠明顯降低HO8910細(xì)胞黏附和遷移能力;降低MMP9和MMP2的表達(dá);減少細(xì)胞TNF-α、NF-κB/p65的表達(dá),RIB可以減弱DDP的作用。結(jié)論干擾FIB代謝途徑能夠增強DDP對卵巢癌HO8910細(xì)胞黏附和遷移能力的作用,該作用與抑制TNF-α/NF-κB途徑有關(guān)。
[Abstract]:Objective to study the effect of riboflavin on the adhesion and migration of ovarian cancer HO8910 cell line by cisplatin DDP in vitro. Methods RIB was interfered with by lentivirus packaging riboflavin transporter 2(RFT2)shRNA interference vector and RIB competitive inhibitor chlorpromazine. Metabolic pathway. HO8910 ovarian cancer cells were randomly divided into two groups: blank control group, control group, RFT2 shRNA group, 50 渭 mol shRNA group) group, 20 渭 mol shRNA shRNA DDP group, chl DDP group and DDP RIB(20 渭 mol Lf-1 group, the cells in each group were treated for 48 h. Cell adhesion assay and Transwell method Western blot was used to detect the expression of VEGF MMP9 and MMP2. The expression of TNF- 偽 and NF- 魏 B/p65 was detected by confocal laser. Results compared with the control group, RFT2 shRNA or CHL combined with DDP could significantly reduce the adhesion and migration ability of HO8910 cells, decrease the expression of MMP9 and MMP2, and decrease the expression of MMP9 and MMP2. Conclusion interfering with the metabolic pathway of FIB can enhance the ability of DDP to adhesion and migration of ovarian cancer HO8910 cells, which is related to the inhibition of TNF- 偽 / NF- 魏 B pathway.
【作者單位】： 麗水學(xué)院醫(yī)學(xué)與健康學(xué)院;麗水市婦幼保健院產(chǎn)科;
【基金】：浙江省醫(yī)藥衛(wèi)生科學(xué)研究基金資助項目(2013KYA236) 麗水學(xué)院博士科研啟動基金資助(QD1229)
【分類號】：R737.31
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本文編號：1663912