本文選題：CXCL10　切入點(diǎn)：子癇前期　出處：《山西醫(yī)科大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：通過(guò)研究正常孕晚期婦女和子癇前期重度患者胎盤組織中趨化因子CXCL10的表達(dá)情況，探討CXCL10在子癇前期患者胎盤組織中的表達(dá)及意義。 方法：選取30例正常孕晚期婦女和30例子癇前期患者作為對(duì)照組和研究組，采用原位雜交方法測(cè)定兩組胎盤組織中CXCL10mRNA的表達(dá)水平，并且應(yīng)用免疫組化方法檢測(cè)兩組胎盤組織中CXCL10的表達(dá)水平，通過(guò)醫(yī)學(xué)圖像分析系統(tǒng)對(duì)實(shí)驗(yàn)結(jié)果進(jìn)行定量檢測(cè)并作比較。數(shù)據(jù)資料應(yīng)用SPSS17.0統(tǒng)計(jì)軟件進(jìn)行統(tǒng)計(jì)學(xué)處理，，兩樣本均數(shù)比較采用獨(dú)立樣本t檢驗(yàn)。 結(jié)果： （1）原位雜交方法顯示CXCL10mRNA在正常孕晚期婦女和子癇前期患者胎盤組織的滋養(yǎng)細(xì)胞胞漿中有陽(yáng)性表達(dá)，CXCL10mRNA在子癇前期患者胎盤組織中的表達(dá)（平均灰度值：144.52±3.46）明顯高于其在正常孕晚期婦女胎盤組織中的表達(dá)（平均灰度值：156.04±2.78），差異有統(tǒng)計(jì)學(xué)意義（P＜0.05）； （2）免疫組化方法顯示CXCL10在正常孕晚期婦女和子癇前期患者胎盤組織的滋養(yǎng)細(xì)胞胞漿中有陽(yáng)性表達(dá)，CXCL10在子癇前期患者胎盤組織中的表達(dá)（平均灰度值：98.29±3.58）明顯高于其在正常孕晚期婦女胎盤組織中的表達(dá)（平均灰度值：118.90±3.29），差異有統(tǒng)計(jì)學(xué)意義（P＜0.05）。 結(jié)論：原位雜交及免疫組化方法均顯示CXCL10在正常妊娠組和子癇前期患者胎盤組織滋養(yǎng)細(xì)胞胞漿中有表達(dá)。兩種實(shí)驗(yàn)方法均提示CXCL10在子癇前期患者胎盤組織中的陽(yáng)性表達(dá)高于正常妊娠組，提示CXCL10的升高與子癇前期的發(fā)病機(jī)制有關(guān)。
[Abstract]:Objective: to study the expression of chemokine CXCL10 in placenta of normal women with late pregnancy and severe preeclampsia, and to explore the expression and significance of CXCL10 in placenta of preeclampsia. Methods: 30 normal women with late pregnancy and 30 patients with preeclampsia were selected as control group and study group. In situ hybridization was used to detect the expression of CXCL10mRNA in placenta of the two groups. The expression of CXCL10 in two groups of placentas was detected by immunohistochemical method, and the experimental results were quantitatively detected and compared by the medical image analysis system. The data were processed statistically by SPSS17.0 statistical software. The mean of two samples was compared by independent sample t test. Results:. (1) in situ hybridization method showed that the expression of CXCL10 mRNA in placental cytoplasm of normal pregnant women and preeclampsia patients was significantly higher than that of CXCL10 mRNA in placenta of preeclampsia (mean gray value: 144.52 鹵3.46). The expression in placenta of normal women in late pregnancy (mean gray value: 156.04 鹵2.78) was significantly different (P < 0.05). (2) Immunohistochemical method showed that the expression of CXCL10 in placental cytoplasm of normal women with late pregnancy and preeclampsia was significantly higher than that of CXCL10 in placenta of preeclampsia (mean gray value: 98.29 鹵3.58). The expression (mean gray value: 118.90 鹵3.29) of placenta was significantly higher than that of normal women (P < 0.05) during the third trimester of pregnancy. Conclusion: the expression of CXCL10 in placental trophoblast cytoplasm of normal pregnancy group and preeclampsia group is demonstrated by in situ hybridization and immunohistochemistry. Both methods suggest that CXCL10 is expressed in placenta of preeclampsia. The positive expression was higher in normal pregnancy group than in normal pregnancy group. It is suggested that the increase of CXCL10 is related to the pathogenesis of preeclampsia.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R714.244

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 朱雪娟;宋建華;張學(xué)光;孫華;張光波;胡曉紅;薛愛(ài)麗;;干擾素-γ誘導(dǎo)蛋白10在子癇前期大鼠模型的表達(dá)[J];現(xiàn)代婦產(chǎn)科進(jìn)展;2011年04期

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