發(fā)布時(shí)間：2018-01-19 06:24

  本文關(guān)鍵詞： 離心法 梯密度 精子 受精　出處：《中國男科學(xué)雜志》2016年08期 　論文類型：期刊論文

【摘要】：目的探究梯度密度離心中離心力、時(shí)間和梯度液體積對前向運(yùn)動(dòng)精子回收率和受精潛能的影響。方法實(shí)驗(yàn)分新鮮精液組和冷凍精液組各30例。每例精液充分混勻后,平均分為4份,進(jìn)行下列各組實(shí)驗(yàn):(1)組1為80%和40%的梯度離心液各0.5m L,300×g,離心20min;(2)組2為80%和40%的梯度離心液各1.0m L,300×g,離心20min;(3)組3為80%和40%的梯度離心液各1.0m L,1 200×g,離心5min;(4)組4為80%和40%的梯度離心液各0.5m L,1 200×g,離心5min。分別檢測處理前后每組精液濃度、前向運(yùn)動(dòng)精子百分率、正常形態(tài)率以及精子DNA碎片率,以分析離心力、時(shí)間和梯度液體積對精子前向運(yùn)動(dòng)精子回收率和受精潛能的影響。結(jié)果無論是新鮮精液,還是冷凍精液,組1、組3和組4前向運(yùn)動(dòng)精子的回收率和精子的正常形態(tài)率以及精子的DNA碎片率與組2比較無明顯差異(P0.05)。結(jié)論將現(xiàn)行的300×g,離心20min的離心方法,改為1 200×g,離心5min,梯度離心液體積從1.0mL降低至0.5mL,并不顯著影響精子正常形態(tài)率、前向運(yùn)動(dòng)精子的回收率和精子的受精潛能,是可行的。
[Abstract]:Objective to explore the centrifugal force of gradient density. Effects of time and gradient liquid volume on the recovery rate and fertilization potential of forward motile spermatozoa. Methods 30 cases of fresh semen group and 30 cases of frozen semen group were divided into 4 parts on average after each case was fully mixed. The following experiments were carried out in each group: group 1: 80% and 40% gradient centrifugation solution, 0.5 mL / L, 300 脳 g, centrifugation for 20 min; Group 2: 80% and 40% gradient centrifuge solution, 1.0 mL / g, respectively, for 20 min; The gradient centrifuge solution of 80% and 40% in group 3 was 1.0 mL / g each for 5 mins. Group 4: 80% and 40% gradient centrifugals (0.5 mL / g, 1 200 脳 g, respectively) were centrifuged for 5 min. The semen concentration and the percentage of forward motile sperm in each group were measured before and after treatment. Normal morphology rate and sperm DNA fragment rate were used to analyze the effects of centrifugal force, time and gradient liquid volume on the recovery rate and fertilization potential of forward motile spermatozoa. The results were as follows: fresh semen and frozen semen. There was no significant difference between group 1, group 3 and group 4 in the recovery of forward motile sperm, the normal morphology rate of sperm and the rate of DNA fragment in sperm compared with group 2 (P 0.05). The centrifugation method for 20 min was changed to 1 200 脳 g and centrifuged for 5 min. The volume of gradient centrifuge decreased from 1.0 mL to 0.5 mL, and the normal morphology rate of sperm was not significantly affected. The recovery rate of forward motile sperm and the fertilization potential of spermatozoa are feasible.
【作者單位】： 國家衛(wèi)生計(jì)生委男性生殖與遺傳重點(diǎn)實(shí)驗(yàn)室廣東省計(jì)劃生育科學(xué)技術(shù)研究所生殖中心;
【基金】：廣東省計(jì)劃生育委員會(huì)研究項(xiàng)目(編號(hào):20132010) 廣東省醫(yī)學(xué)科研基金項(xiàng)目(編號(hào):A2014195)資助
【分類號(hào)】：R446.19;R714.8
【正文快照】： 在輔助生育技術(shù)中,技術(shù)人員要通過相應(yīng)的精濃度、精子活動(dòng)率等精液常規(guī)分析。子分離技術(shù)除去不活動(dòng)的、形態(tài)不正常、不成熟的精(四)用Diffquik染色法進(jìn)行精子形態(tài)學(xué)分析子以及精漿,獲得高質(zhì)量的精子進(jìn)行體外受精。精子依照世界衛(wèi)生組織人類精液檢查與處理實(shí)驗(yàn)室分離技術(shù)的效率

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