【摘要】：近年來,隨著人民生活水平的不斷提高,飲食習(xí)慣和飲食結(jié)構(gòu)的改變以及人口老齡化,我國直結(jié)腸癌的發(fā)病率和死亡率均呈現(xiàn)上升趨勢(shì)。直結(jié)腸癌發(fā)病率和致死率在消化系統(tǒng)惡性腫瘤中僅次于胃癌,食管癌,大多數(shù)患者發(fā)現(xiàn)時(shí)已屬于中晚期。 到目前為止,CEA、CA19-9、CA24-2是公認(rèn)的最可信的與直結(jié)腸癌相關(guān)的血清腫瘤標(biāo)志物,但是診斷敏感性和特異性并不高。本實(shí)驗(yàn)研究目的是對(duì)比直結(jié)腸癌病人、健康志愿者血清白蛋白與自旋探針結(jié)合能力,并分析自旋探針標(biāo)記的血清白蛋白電子順磁共振波譜在直結(jié)腸癌診斷中的價(jià)值。 隨機(jī)選取2012年10月至2013年3月收治的141例直結(jié)腸癌病人和180例健康志愿者的血清,選用16Doxyl硬脂酸作為自旋探針,通過電子順磁共振波譜儀獲取直結(jié)腸癌病人、健康志愿者血清白蛋白與自旋探針結(jié)合的波譜圖譜。利用從圖譜中獲得的五個(gè)參數(shù)(△H1△H2△H3△H4△H5,相對(duì)線寬和峰寬),結(jié)合統(tǒng)計(jì)學(xué)中的判別分析方法,建立判別函數(shù)。 直結(jié)腸癌患者血清白蛋白與自旋探針的結(jié)合能力,與健康人的相比,有明顯的差異。通過對(duì)波譜分析,從波譜中獲得5種參數(shù),將這5種參數(shù)應(yīng)用到判別分析的統(tǒng)計(jì)學(xué)方法中,得到判別分析的函數(shù)：F1=338.904△H1+88328.145△H2-103.109△H3+96423.334△H4+265.178△H5-255051.924; F2=328.375AH1+88226.653△H2-116.781△H3+96324.452△H4+259.369△H5-254166.845。診斷特異性和敏感性分別為87.9%和86.1%,證實(shí)篩檢試驗(yàn)有很大的診斷價(jià)值。 直結(jié)腸癌病人、健康人血清白蛋白與自旋探針結(jié)合能力不同,腫瘤相關(guān)的血清白蛋白的變化可以通過自旋標(biāo)記的電子順磁共振波譜來評(píng)價(jià),自旋探針標(biāo)記的血清白蛋白電子順磁共振波譜診斷直結(jié)腸癌具有較高的診斷特異性和敏感性,該方法有可能成為臨床新的直結(jié)腸癌實(shí)驗(yàn)室診斷方法。
[Abstract]:In recent years, with the improvement of people's living standard, the change of diet habits and diet structure, and the aging of population, the morbidity and mortality of colon cancer in China are on the rise. The morbidity and mortality of colon cancer are second only to gastric cancer and esophageal carcinoma in digestive system malignant tumor. So far, CA19-9 CA24-2 is recognized as the most reliable serum tumor marker associated with colorectal cancer, but the diagnostic sensitivity and specificity are not high. The aim of this study was to compare the binding ability of serum albumin with spin probe in patients with colon cancer and to analyze the value of electron paramagnetic resonance spectroscopy (EPR) of serum albumin labeled with spin probe in the diagnosis of colon cancer. Serum samples of 141 patients with colorectal cancer and 180 healthy volunteers were randomly selected from October 2012 to March 2013. 16Doxyl stearic acid was used as spin probe to obtain colorectal cancer patients by electron paramagnetic resonance spectroscopy. Spectrogram of the binding of serum albumin with spin probe in healthy volunteers. Using the five parameters (H 1H 2H 3H 4H 5, relative linewidth and peak width) obtained from the map and combining the discriminant analysis method in statistics, the discriminant function was established. The binding ability of serum albumin with spin probe in colon cancer patients was significantly different from that in healthy people. Through the spectral analysis, five parameters were obtained from the spectrum, and the five parameters were applied to the statistical method of discriminant analysis. The function of discriminant analysis was obtained, the function of discriminant analysis was 338.904 H1 88328.145 H2-103.109 H3 96423.334 H4 265.178 H5-255051.924, F2=328.375AH1 88226.653 H2-116.781 H3 96324.452 H4 259.369 H5-254166.845. The diagnostic specificity and sensitivity were 87.9% and 86.1% respectively. In colorectal cancer patients, the binding ability of serum albumin to spin probe is different. The change of serum albumin associated with tumor can be evaluated by spin labeled electron paramagnetic resonance spectroscopy. Electron paramagnetic resonance spectroscopy of serum albumin labeled with spin probe has high diagnostic specificity and sensitivity, and this method may become a new laboratory diagnostic method for colorectal cancer.
【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R735.34;R445.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 宋風(fēng)忠;谷令彪;朱曉蕊;;基于ESR技術(shù)的煙氣中自由基分析的研究進(jìn)展[J];安徽農(nóng)業(yè)科學(xué);2011年14期

2 胡明華;翁媛英;吳玉林;陳再智;;多層螺旋CT對(duì)直腸癌和結(jié)腸癌的診斷價(jià)值[J];放射學(xué)實(shí)踐;2007年08期

3 王長振;吳可;;位置定向自旋標(biāo)記——電子順磁共振技術(shù)在蛋白質(zhì)結(jié)構(gòu)研究中的應(yīng)用[J];軍事醫(yī)學(xué)科學(xué)院院刊;2006年05期

4 李俊;盧文喜;辛欣;王偉卓;;判別分析法在飲用水源地水質(zhì)評(píng)價(jià)中的應(yīng)用[J];人民黃河;2009年02期

5 王翠平;葉柳;謝安建;李廣;李愛俠;張子云;張惠;;電子順磁共振技術(shù)應(yīng)用及進(jìn)展[J];實(shí)驗(yàn)室研究與探索;2013年05期

6 郭來敬,張志仁,陳東輝,王璞;電子順磁共振檢測(cè)心肌缺血再灌產(chǎn)生的氧自由基及其保護(hù)的實(shí)驗(yàn)研究[J];心肺血管病雜志;2000年01期

7 楊磊;許衛(wèi);杜祥穎;于春水;郝帥營;;腹部CTA和CTC及融合圖像在結(jié)、直腸腫瘤的臨床應(yīng)用價(jià)值[J];醫(yī)學(xué)影像學(xué)雜志;2008年12期

8 李曉毅;Bayes判別分析及其在疾病診斷中的應(yīng)用[J];中國衛(wèi)生統(tǒng)計(jì);2004年06期

9 鄭樹;蔡善榮;張?zhí)K展;;重視結(jié)直腸癌高危人群的篩查[J];中華消化雜志;2006年06期

10 薛鴻慶;電子順磁共振[J];自然雜志;1981年12期

，

本文編號(hào)：2256806