【摘要】：目的制備載水飛薊賓熱敏脂質(zhì)體-微泡SonoVue復(fù)合體并進行質(zhì)量分析。方法以薄膜旋蒸法制備載水飛薊賓熱敏脂質(zhì)體,然后利用戊二醛偶聯(lián)法將超聲微泡SonoVue與載藥脂質(zhì)體相連接,通過紫外分光光度法檢測熱敏脂質(zhì)體藥物包封率及不同溫度下釋藥特性。通過激光共聚焦顯微鏡檢測載藥脂質(zhì)體與微泡的偶聯(lián)率。結(jié)果載水飛薊賓熱敏脂質(zhì)體-微泡SonoVue復(fù)合體粒徑(2 724±559.9)nm,藥物包封率(71.0±8.3)%,41℃時藥物平均釋放率明顯升高,達到(55.6±7.6)%。偶聯(lián)后,載藥脂質(zhì)體在微泡周圍聚集,熱敏脂質(zhì)體與微泡偶聯(lián)率為(90.0±4.9)%。結(jié)論載水飛薊素?zé)崦糁|(zhì)體-微泡SonoVue復(fù)合體具有良好的偶聯(lián)率、較高的藥物包封率及控溫釋藥特性,為藥物遞送提供一種新的載體。
[Abstract]:Objective to prepare silybin-loaded thermosensitive liposome-microbubble SonoVue complex and analyze its quality. Methods Silybin loaded thermosensitive liposomes were prepared by thin film spinning method. The ultrasonic microbubble SonoVue was linked to the drug-loaded liposome by glutaraldehyde coupling method. The encapsulation efficiency of thermosensitive liposomes and the drug release characteristics at different temperatures were determined by UV spectrophotometry. The coupling rate between liposomes and microbubbles was measured by confocal laser microscopy. Results the particle size of silibine-microbubble SonoVue complex was (2 724 鹵559.9) nm, and the drug encapsulation efficiency was (71. 0 鹵8. 3) at 41 鈩，

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