發(fā)布時(shí)間：2018-08-07 18:54

【摘要】：經(jīng)顱超聲刺激是新近發(fā)展起來的一種大腦功能刺激技術(shù),該技術(shù)通過透過完整的顱骨傳遞超聲能量，能無創(chuàng)地調(diào)制大腦的神經(jīng)活動(dòng)。本文首先介紹了超聲在生物醫(yī)學(xué)中的應(yīng)用以及經(jīng)顱超聲刺激技術(shù)的研究現(xiàn)狀及意義，，然后介紹了經(jīng)顱超聲刺激系統(tǒng)的關(guān)鍵參數(shù)以及提高空間分辨率的方法。建立經(jīng)顱超聲系統(tǒng)之后，首先通過經(jīng)顱脈沖超聲刺激SD大鼠的motor及Barrel皮層，研究不同的超聲刺激參數(shù)調(diào)解大腦神經(jīng)活動(dòng)的能力；隨后，基于中風(fēng)模型建立之后立即給予超聲刺激能夠有效地對(duì)于保護(hù)缺血性腦損傷的假設(shè)，設(shè)計(jì)實(shí)驗(yàn)利用經(jīng)顱超聲刺激SD大鼠遠(yuǎn)端中動(dòng)脈缺血性模型，通過動(dòng)物行為學(xué)評(píng)定運(yùn)動(dòng)狀況（刺激組(n=10,5.5±2.5)對(duì)照組(n=10,10.5±1.4)）、TTC染色測定皮層組織梗死面積比（刺激組(13.78%±7.39%)對(duì)照組(43.39%±2.33%)）、內(nèi)源光成像技術(shù)評(píng)估腦功能等來評(píng)價(jià)經(jīng)顱超聲刺激對(duì)缺血性腦損傷的保護(hù)效果，最后通過激光散斑成像技術(shù)觀測感興趣區(qū)域的血供以及免疫組化測定細(xì)胞炎癥來研究超聲的腦保護(hù)的潛在作用機(jī)制，提出了血流灌注增強(qiáng)和炎癥反應(yīng)減弱兩種可能的保護(hù)作用機(jī)制。本項(xiàng)目的研究有望揭示經(jīng)顱超聲刺激在卒中后的早期神經(jīng)保護(hù)的作用機(jī)理，并為缺血性腦卒中的神經(jīng)保護(hù)提供新的治療康復(fù)方法。
[Abstract]:Transcranial ultrasound stimulation (TUS) is a newly developed technique of brain function stimulation, which can modulate the neural activity of the brain noninvasively by transmitting ultrasonic energy through the intact skull. In this paper, the application of ultrasound in biomedicine and the research status and significance of transcranial ultrasound stimulation are introduced, then the key parameters of transcranial ultrasound stimulation system and the method of improving spatial resolution are introduced. After the establishment of transcranial ultrasound system, the motor and Barrel cortex of SD rats were stimulated by transcranial pulse ultrasound first to study the ability of different ultrasound stimulation parameters to regulate the brain nerve activity. Based on the hypothesis that ultrasound stimulation can effectively protect ischemic brain injury immediately after the establishment of stroke model, we designed an ischemic model of distal middle artery of SD rats by transcranial ultrasound stimulation. The infarct area ratio of cortical tissue was measured by animal behavior assessment (stimulation group (10 5 鹵2 5 鹵2 5) control group (n 10 10 5 鹵1 4) and control group (13 78% 鹵7 39%) and control group (43 39% 鹵2 33%), endogenous light imaging technique) to evaluate the effect of transcranial ultrasound stimulation on ischemic ischemia. The protective effect of brain injury, Finally, the potential mechanism of ultrasonic brain protection was studied by using laser speckle imaging technique to observe the blood supply in the region of interest and to determine the cellular inflammation by immunohistochemistry. Two possible protective mechanisms of increased blood perfusion and weakened inflammatory response were proposed. This project is expected to reveal the mechanism of transcranial ultrasound stimulation in early post-stroke neuroprotection and provide a new method for the treatment and rehabilitation of ischemic stroke.
【學(xué)位授予單位】：上海交通大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R445.1;R743.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 崔雪蓮;張理義;徐志熊;;經(jīng)顱磁刺激在中風(fēng)康復(fù)中的應(yīng)用進(jìn)展[J];國際神經(jīng)病學(xué)神經(jīng)外科學(xué)雜志;2007年02期

2 王培芝;王巍;徐維維;王翠平;;低頻重復(fù)經(jīng)顱磁刺激治療腦卒中后抑郁療效觀察[J];河北醫(yī)藥;2008年09期

3 張瑩;舒兆瑞;沈梅紅;;線栓法制備腦缺血再灌注的影響因素及探討[J];遼寧中醫(yī)藥大學(xué)學(xué)報(bào);2012年12期

4 劉敬霞,李建生;線栓法制備大鼠局灶性腦缺血模型的進(jìn)展及評(píng)價(jià)[J];中國比較醫(yī)學(xué)雜志;2004年06期

本文編號(hào)：2171035