本文選題：核磁共振 + 生物分子��； 參考：《中國組織工程研究》2015年46期

【摘要】：背景:目前研究發(fā)現800 m跑的代謝特征不是十分明晰,為800 m的運動訓練造成了很大的阻礙。目的:了解800 m跑項目的物質和能量代謝特征,提高800 m跑訓練的科學化水平,驗證基于核磁共振的尿液代謝組學技術在運動訓練監(jiān)測方面的可行性。方法:對某高校運動訓練專業(yè)7名中跑2級運動員進行一次極限強度800 m跑,結合人體代謝數據庫(HMDB)等數據庫,對運動前、后的尿液進行代謝組學相關分析。結果與結論:OPLS-DA模型顯示運動前和運動后樣本的代謝模式呈現簇類分布。造成這一變化的特征性代謝物主要有亮氨酸、乳酸、肌酐、琥珀酸、次黃嘌呤、腺苷等代謝物。通過分析可以推斷出800 m跑是以糖酵解供能為主、磷酸原供能和有氧氧化供能共同參與的運動項目,800 m跑后運動員氨基酸代謝、甲烷代謝等代謝過程較為旺盛,而且在800 m跑的過程中會出現一定程度的氧化應激。結果說明基于核磁共振的尿液代謝組學技術有無創(chuàng)性、靈敏度高、樣品需求量少、處理簡單等優(yōu)勢。
[Abstract]:Background: at present, it is found that the metabolic characteristics of 800m running are not very clear, which is a great obstacle for 800m sports training. Objective: to understand the characteristics of substance and energy metabolism in 800m running events, improve the scientific level of 800m running training, and verify the feasibility of urine metabonomics technology based on nuclear magnetic resonance (NMR) in monitoring exercise training. Methods: seven middle and second grade athletes in a certain university were run with the limit strength of 800 m, and the urine before and after exercise was analyzed with the database of Human body Metabolism Database (HMDB) and other databases. Results and conclusions the OPLS-DA model showed that the metabolic patterns of pre-exercise and post-exercise samples showed cluster distribution. The main metabolites responsible for this change are leucine, lactic acid, creatinine, succinic acid, Hypoxanthine, adenosine and other metabolites. It can be inferred from the analysis that the main metabolic process of 800 m running is glycolysis, the metabolism of amino acid, methane and so on after 800 m running. And there will be a certain degree of oxidative stress during the 800 m run. The results showed that the urinary metabolomics technique based on nuclear magnetic resonance had the advantages of noninvasive, high sensitivity, less sample demand and simple treatment.
【作者單位】： 上海體育學院;
【基金】：上海市科學技術委員會科研計劃項目(13490503700)《運動員不同類型運動性疲勞代謝組學圖譜的建立及針灸調控》~~
【分類號】：R445.2;R87

【參考文獻】

相關期刊論文 前2條

1 戴偉東;張鳳霞;賈振華;魏聰;高鵬;路鑫;吳以嶺;許國旺;;基于液相色譜-質譜聯(lián)用技術的代謝組學方法用于中藥通心絡和人參對過度疲勞大鼠干預作用的評價[J];色譜;2011年11期

2 李江華;王智慧;朱小娟;葛耀軍;;代謝組學運動選材與傳統(tǒng)生化選材方法的比較[J];體育學刊;2013年01期

【共引文獻】

相關期刊論文 前10條

1 董培智;吳賢波;岑國棟;高永翔;;生物質譜技術在中藥研究中的應用[J];中國醫(yī)藥科學;2013年01期

2 劉健;萬磊;黃傳兵;汪元;曹云祥;劉磊;程園園;馮云霞;孫s，

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