本文選題：磁共振成像 + 動態(tài)對比增強掃描　； 參考：《放射學(xué)實踐》2015年04期

【摘要】：目的:探討基于Reference-Region模型的DCE-MRI評價恩度對兔VX2骨腫瘤抗血管生成療效的可行性。方法:將成功建立脛骨VX2腫瘤模型(軟組織腫塊直徑1.0cm)的20只兔子隨機(jī)分成對照組(n=10)和實驗組(n=10),分別于給藥前、連續(xù)給藥14天后(對照組藥物為生理鹽水;實驗組藥物為恩度,藥物濃度為1.5mg/8mL)行DCE-MRI掃描,使用Referrence Region模型獲得腫瘤組織的對比劑容積轉(zhuǎn)移常量Ktrans和反流速率常數(shù)Kep。于第14天掃描結(jié)束后處死動物,取腫瘤外周及中心區(qū)域的組織進(jìn)行免疫組化染色檢測,得到腫瘤不同區(qū)域的微血管密度(MVD)及血管內(nèi)皮生長因子(VEGF)的表達(dá)情況,將DCE-MRI各參數(shù)與免疫組化結(jié)果進(jìn)行相關(guān)分析。結(jié)果:對照組中腫瘤外周區(qū)域和中心區(qū)域的Ktrans值在治療前分別為(32.58±3.10)和(28.50±3.54)min-1,治療后分別為(37.66±2.78)和(34.20±3.39)min-1;實驗組中腫瘤組織的外周區(qū)域和中心區(qū)域的Ktrans值在治療前分別為(27.70±4.75)和(23.90±4.40)min-1,在治療后分別為(22.20±4.29)和(18.30±4.23)min-1。實驗組VX2骨腫瘤外周區(qū)域與中心區(qū)域的Ktrans值、MVD值及VEGF表達(dá)情況間的差異均有統(tǒng)計學(xué)意義(P0.05),Kep值的差異無統(tǒng)計學(xué)意義(P0.05)。實驗組治療后腫瘤外周和中心區(qū)域的VEGF表達(dá)、MVD值與Ktrans值間均呈正相關(guān)關(guān)系(r值分別為0.924、0.945、0.848和0.909,P0.01);與Kep值間均無顯著相關(guān)性(r值分別為0.022、0.162、0.219和0.042,P0.01)。結(jié)論:兔VX2骨腫瘤模型的血流灌注空間分布具有異質(zhì)性;基于Reference模型的DCE-MRI定量參數(shù)Ktrans可以評價恩度對兔VX2骨腫瘤抗血管生成的療效。
[Abstract]:Objective: To explore the feasibility of DCE-MRI evaluation based on Reference-Region model for anti angiogenesis effect of rabbit VX2 bone tumor. Methods: 20 rabbits were randomly divided into the control group (n=10) and the experimental group (n =10) to establish the VX2 tumor model of the tibia (the diameter of the soft tissue mass 1.0cm). The control group was given 14 days after administration (the control group). DCE-MRI scanning was performed in the experimental group, the drug concentration was 1.5mg/8mL) and the Referrence Region model was used to obtain the volume transfer constant Ktrans of the tumor tissue and the reflux rate constant Kep. at the end of the fourteenth day scan. The expression of microvascular density (MVD) and vascular endothelial growth factor (VEGF) in different regions of the tumor were measured. The correlation between the parameters of DCE-MRI and the results of immunohistochemistry were analyzed. Results: the Ktrans values of the peripheral and central regions of the tumor were (32.58 + 3.10) and (28.50 + 3.54) min-1 respectively in the control group before treatment, respectively (3 7.66 + 2.78) and (34.20 + 3.39) min-1, the Ktrans values of the peripheral and central region of the tumor tissue in the experimental group were (27.70 + 4.75) and (23.90 + 4.40) min-1 respectively before treatment. The Ktrans value, MVD value and VEGF expression of the peripheral region and the central region of the VX2 bone swelling tumor in the experimental group of (22.20 + 4.29) and (18.30 + 4.23)) were respectively after the treatment. There was no statistical significance (P0.05), and there was no significant difference in the value of Kep (P0.05). The VEGF expression in the peripheral and central region of the experimental group was positively correlated with the value of MVD and Ktrans (r value was 0.924,0.945,0.848 and 0.909, P0.01), and there was no significant correlation with Kep values (R is 0.022,0.162,0.219 and 0., respectively) 042, P0.01) conclusion: the spatial distribution of blood perfusion in rabbit VX2 bone tumor model is heterogeneous, and the DCE-MRI quantitative parameter Ktrans based on Reference model can evaluate the effect of grace on the anti angiogenesis of rabbit VX2 bone tumor.
【作者單位】： 武漢大學(xué)人民醫(yī)院放射科;
【基金】：湖北省自然科學(xué)基金資助項目(2013CFB242) 湖北省衛(wèi)生廳科研資助項目(JX6B68)
【分類號】：R445.2;R738.1

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本文編號：1954303