本文選題：人參皂苷 + 造血干細(xì)胞��； 參考：《山東醫(yī)藥》2015年08期

【摘要】：目的觀察人參總皂苷預(yù)處理對(duì)電離輻射下人造血干細(xì)胞(HPCs)的保護(hù)作用,并探討其作用機(jī)制。方法將HPCs隨機(jī)分為對(duì)照組和觀察組,對(duì)照組不處理,觀察組予以人參皂苷5.0μmol/L培養(yǎng)24 h,分別用劑量為0、1、2、5 Gy的X線對(duì)兩組細(xì)胞進(jìn)行照射。MTT法測(cè)算細(xì)胞存活率,流式細(xì)胞儀測(cè)定輻射后24 h細(xì)胞內(nèi)的活性氧(ROS),Western blot法測(cè)定細(xì)胞內(nèi)的Caspase-3和Nrf-2。結(jié)果隨著輻射劑量的增加,兩組細(xì)胞存活率均逐漸下降(P均0.05),細(xì)胞內(nèi)ROS活性增強(qiáng)、Caspase-3表達(dá)增加、Nrf-2表達(dá)減少(P均0.05);與相同照射劑量對(duì)照組比較,觀察組細(xì)胞存活率增加(P均0.05),細(xì)胞內(nèi)ROS活性減弱、Caspase-3表達(dá)減少、Nrf-2表達(dá)增加(P均0.05)。結(jié)論人參皂苷對(duì)電離輻射下的HPCs具有保護(hù)作用,該作用與其抗凋亡和抑制氧化應(yīng)激有關(guān)。
[Abstract]:Objective to observe the protective effect of ginsenoside pretreatment on human hematopoietic stem cell (HPCs) induced by ionizing radiation and its mechanism. Methods HPCs was randomly divided into control group and observation group. The control group was treated with ginsenoside 5.0 渭 mol/L for 24 hours. The intracellular Caspase-3 and Nrf-2 were determined by flow cytometry 24 h after irradiation. Results with the increase of radiation dose, the survival rate of both groups decreased gradually (P 0.05), and the expression of Caspase-3 increased with the increase of ROS activity in both groups. In the observation group, the cell survival rate was increased (P < 0.05), and the expression of Caspase-3 was decreased, and the expression of nrf-2 was increased (P < 0.05). Conclusion ginsenoside has protective effect on HPCs induced by ionizing radiation, which is related to its anti-apoptosis and inhibition of oxidative stress.
【作者單位】： 陜西省人民醫(yī)院;
【基金】：陜西省自然科學(xué)基金資助項(xiàng)目(S2014JC11060)
【分類號(hào)】：R730.55

【共引文獻(xiàn)】

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