本文選題：超聲造影 + 土三七��； 參考：《中南大學》2014年碩士論文

【摘要】：第一部分土三七誘導大鼠HSOS模型的建立與評價 目的：采用PBS、土三七水煎液大鼠灌胃,模擬臨床患者服藥情況,建立穩(wěn)定、可復制的且能有效模擬臨床發(fā)病過程的大鼠HSOS模型,并給予綜合評價。 方法：30只健康雄性SD大鼠,隨機分為兩組：PBS正常對照組10只和土三七實驗組20只。土三七組給予土三七水煎液,18.18ml/kg/d,對照組給予等容積PBS,每日一次,連續(xù)28天,末次給藥后,次日清晨各組大鼠給予安樂死。取大鼠血清,檢測肝功能情況,留取肝組織行HE染色和MASSON染色,采用Deleve評分標準進行病理學評分。 結果： 1.土三七組有18只大鼠成模,PBS對照組10只大鼠均未誘導出HSOS。 2.與PBS正常對照組比較,土三七組大鼠肝指數、ALT、AST、TBIL均明顯升高,ALB明顯下降(P0.05) 3.根據修改后的Deleve評分標準,土三七組大鼠多為中-重度HSOS改變。 結論：本實驗成功建立了土三七水煎液致肝竇阻塞綜合征大鼠模型,為進一步研究HSOS發(fā)病機制奠定了良好的實驗基礎。 第二部分超聲造影對土三七誘導的HSOS的診斷價值 目的：將超聲造影與定量分析技術相結合,應用于大鼠HSOS模型,討論超聲造影技術在HSOS中的診斷價值。 方法：PBS組和土三七組大鼠連續(xù)灌胃28天后,進行超聲造影檢查,使用定量分析軟件對造影圖像進行分析,繪制時間-強度曲線,得出各項定量參數。采用t檢驗法進行統(tǒng)計學分析,對兩組大鼠各項定量參數進行比較,以P0.05認為差異有統(tǒng)計學意義。 結果： 1.超聲造影圖像中土三七組大鼠肝實質強化不均勻,可見多處小片狀高增強區(qū)域,甚至出現大片無灌注區(qū),整個肝臟呈“地圖樣”強化,門靜脈造影劑充填緩慢,肝靜脈強化減弱或不強化。 2.時間-強度曲線中各項定量參數分析顯示,與對照組比較,實驗組大鼠達峰時間(TTP)、峰值減半時間(T1/2)、曲線上升支斜率(K1)、曲線下降支斜率(K2)延長,峰值強度(PI)減小(P0.05),其余參數如強度增量(PI-BI)、曲線下面積(AREA)兩組間差異均無統(tǒng)計學意義(P0.05)。 結論：超聲造影與定量分析技術相結合,對HSOS的診斷具有一定的意義。超聲造影有望成為無創(chuàng)傷性診斷HSOS的新指標。
[Abstract]:The first part: establishment and evaluation of HSOS model induced by Panax notoginseng in rats Objective: to establish a stable, replicable and effective HSOS model of rats with PBS- and Panax notoginseng decoction to simulate the drug taking by clinical patients, and to give a comprehensive evaluation. Methods 30 healthy male Sprague-Dawley rats were randomly divided into two groups: control group (n = 10) and control group (n = 20). The rats in the control group were treated with isovolumetric PBSs once a day for 28 days. The rats in each group were euthanized the next morning after the last administration. The liver function was detected in the serum of rats. The liver tissues were stained with HE and MASSON. The pathological scores were evaluated by Deleve scoring standard. Results: 1. No HSOSs were induced in 18 rats of PBS group and 10 rats in control group. 2. Compared with the normal control group of PBS, the liver index of rats in Panax notoginseng group was significantly higher than that in the control group (P 0.05). 3. According to the revised Deleve score, moderate to severe HSOS changes were found in rats of Panax notoginseng group. Conclusion: the rat model of hepatic sinusoidal obstruction syndrome induced by water decoction of Panax notoginseng was successfully established in this experiment, which laid a good experimental foundation for further study on the pathogenesis of HSOS. The value of contrast-enhanced ultrasonography in the diagnosis of HSOS induced by Panax notoginseng Objective: to study the diagnostic value of contrast-enhanced ultrasound (CEUs) in HSOS in rat HSOS model by combining contrast-enhanced ultrasound with quantitative analysis. Methods after 28 days of continuous gavage in the two groups, ultrasound examination was performed, and quantitative analysis software was used to analyze the contrast images. The time-intensity curves were drawn and the quantitative parameters were obtained. T test method was used to carry on the statistical analysis, the two groups of rats were compared with each other quantitative parameters, with P0.05 that the difference was statistically significant. Results: 1. The enhancement of hepatic parenchyma was not uniform in the contrast-enhanced images of the rats in the group of Notoginseng. There were many small and high enhancement areas, even a large area without perfusion. The whole liver was enhanced like a map, and the portal vein contrast agent was filled slowly. Hepatic vein enhancement was weakened or not. 2. The quantitative parameter analysis in the time-intensity curve showed that compared with the control group, the rats in the experimental group had the highest peak time of TTPN, the peak half time of T1 / 2, the slope of the ascending branch of the curve, the slope of the descending branch of the curve, and the slope of the descending branch of the curve (K2). Peak intensity (Pi) decreased P0.05N, but there was no significant difference between the two groups in other parameters, such as intensity increment PI-BII, area under curve area (AREAA), between the two groups, there was no significant difference between the two groups (P 0.05). Conclusion: the combination of contrast-enhanced ultrasound and quantitative analysis is of significance for the diagnosis of HSOS. Contrast-enhanced ultrasonography is expected to be a new index for non-traumatic diagnosis of HSOS.
【學位授予單位】：中南大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R445.1;R575

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