發(fā)布時間：2018-04-10 14:19

  本文選題：乳腺癌 + 同種移植動物模型��； 參考：《中國醫(yī)學科學院學報》2015年06期

【摘要】：目的探討乳腺癌超聲造影(CEUS)增強模式與缺氧誘導因子1α(HIF-1α)表達及微血管密度(MVD)的關系。方法在22只小鼠體內建立同種移植性乳腺癌,于接種后第4、6、7、8、9、10、11天隨機抓取3~4只小鼠進行常規(guī)超聲及CEUS檢查,檢查結束后處死小鼠并使用免疫組織化學方法檢測腫瘤標本中HIF-1α表達及MVD,分析CEUS增強模式與HIF-1α表達及MVD的關系。結果 22只小鼠乳腺癌中瘤體0.05 cm3組5例,CEUS表現(xiàn)為Ⅰ型增強模式,周邊環(huán)狀增強,內部無增強4例,Ⅱ型增強模式,周邊環(huán)狀增強并向病灶內呈點線狀穿入1例;0.05~0.75 cm3組9例,多表現(xiàn)為Ⅲ型增強模式,病灶彌漫性增強,均勻/不均勻5例;0.75 cm3組8例,多表現(xiàn)為Ⅳ型增強模式,周邊環(huán)狀增強,內部結節(jié)狀增強7例。3組瘤體在CEUS增強模式上差異有統(tǒng)計學意義(P0.01),Ⅰ~Ⅳ型CEUS增強模式與腫瘤大小相關(r=0.841,P0.05)。小鼠乳腺癌中HIF-1α表達與增強模式(Ⅰ~Ⅳ型)呈負相關(r=-0.596,P=0.003),與腫瘤大小無相關性(P0.05)。Ⅰ~Ⅳ型CEUS增強模式間MVD均值差異無統(tǒng)計學意義,MVD與腫瘤大小無相關性(P0.05)。結論 CEUS可動態(tài)評估同種移植性小鼠乳腺癌的血管生成,腫瘤增強模式與腫瘤HIF-1α的表達水平相關。
[Abstract]:Objective to investigate the relationship between the enhanced mode of CEUs and the expression of hypoxia inducible factor-1 偽 (HIF-1 偽) and microvessel density (MVD) in breast cancer.Methods the allograft breast cancer was established in 22 mice. On the 4th day after inoculation, 34 mice were randomly picked up for routine ultrasound and CEUS examination.After examination, the mice were killed and the expression of HIF-1 偽 and MVD in tumor specimens were detected by immunohistochemical method. The relationship between CEUS enhancement mode and HIF-1 偽 expression and MVD were analyzed.There were 8 cases in 0. 75 cm3 group with diffuse enhancement, 8 cases in 0. 75 cm3 group, and 8 cases in 0. 75 cm3 group. Most of them showed type 鈪，

本文編號：1731557