本文關(guān)鍵詞： 前列腺癌 慢性前列腺炎 超聲血流動(dòng)力學(xué) 微血管密度　出處：《廣西醫(yī)科大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：本研究旨在通過(guò)經(jīng)直腸彩色多普勒超聲對(duì)前列腺可疑病灶區(qū)血流動(dòng)力學(xué)變化的觀察,并結(jié)合與病理微血管密度(microvascular denstity,MVD)的免疫組化觀察,探討經(jīng)直腸彩色多普勒超聲鑒別診斷前列腺癌和慢性前列腺炎的診斷價(jià)值。 方法：收集臨床疑為前列腺癌患者共61例,經(jīng)直腸超聲掃查前列腺,檢測(cè)前列腺可疑病灶區(qū)血流動(dòng)力學(xué)指數(shù)(Vs、Vd、PI、RI),并進(jìn)行血運(yùn)分級(jí)(0～3級(jí))。并于超聲引導(dǎo)下行前列腺穿刺,獲取病理結(jié)果證實(shí)為前列腺癌38例,慢性前列腺炎23例。點(diǎn)陣列包埋、石蠟切片行HE染色,經(jīng)病理醫(yī)師診斷,用CD34單克隆抗體對(duì)前列腺活檢組織行免疫組織化學(xué)染色,采用Weinder方法對(duì)CD34標(biāo)記的微血管密度進(jìn)行定量計(jì)數(shù)。 結(jié)果：前列腺癌組病灶區(qū)血流動(dòng)力學(xué)指數(shù)(Vs、PI、RI)及血運(yùn)分級(jí)明顯高于慢性前列腺炎組(P0.05)；Vs、PI、RI各指標(biāo)均在前列腺癌(C+D)期和(A+B)期、前列腺癌Gleason評(píng)分分為≥7分高Gleason評(píng)分組和7分低Gleason評(píng)分組兩組中比較有差異性(P均0.05)。血流動(dòng)力學(xué)指數(shù)Vd于各組比較中均無(wú)明顯差異(P0.05)。MVD計(jì)數(shù)在前列腺癌組和慢性前列腺炎組分別為46.70±13.87、34.38±7.28(P0.05)。MVD計(jì)數(shù)在前列腺癌(C+D)期和(A+B)期分別為56.99±12.85、39.97±10.21(P0.05)；在前列腺癌高Gleason評(píng)分組和低Gleason評(píng)分組分別為53.79±13.30、36.96±7.24(P0.05)。前列腺癌病灶區(qū)的血流動(dòng)力學(xué)指數(shù)(Vs、PI、RI)及血運(yùn)分級(jí)與前列腺癌組織MVD呈顯著正相關(guān)(P0.05)。 結(jié)論：經(jīng)直腸彩色多普勒超聲影像顯示的前列腺癌血流動(dòng)力學(xué)指數(shù)(Vs、PI、RI)和血運(yùn)分級(jí)能較好地反映腫瘤組織內(nèi)微血管的變化,且前列腺癌組可疑病灶或血流豐富區(qū)血流動(dòng)力學(xué)指數(shù)(Vs、PI、RI)及血運(yùn)分級(jí)明顯高于慢性前列腺炎組,可作為兩者鑒別診斷的有用指標(biāo)。
[Abstract]:Objective: to observe the changes of hemodynamics in the suspected lesion area of prostate by transrectal color Doppler ultrasound and to observe the microvascular denstitystitystitystitystitystitystitystityus microvascular VDD by immunohistochemistry. To evaluate the diagnostic value of transrectal color Doppler ultrasound in the differential diagnosis of prostate cancer and chronic prostatitis. Methods: a total of 61 patients with suspected prostate cancer were collected. The prostate was examined by transrectal ultrasound, and the hemodynamic index of the suspected lesions was detected. Pathological results were obtained from 38 cases of prostate cancer and 23 cases of chronic prostatitis. The specimens were embedded with dot array and stained with HE on paraffin sections. The biopsy tissues were stained with CD34 monoclonal antibody by immunohistochemical staining after being diagnosed by pathologist. The microvessel density (MVD) labeled with CD34 was counted quantitatively by Weinder method. Results: the regional hemodynamic index (Pi) and the grade of blood circulation in prostate cancer group were significantly higher than those in chronic prostatitis group (P 0.05). The Gleason score of prostate cancer was divided into two groups: high score (鈮，

本文編號(hào)：1497117