【摘要】：目的： 1、探討新生兒高膽紅素血癥與巨細(xì)胞病毒(HCMV)感染的相關(guān)性及臨床意義。 2、進(jìn)一步探討熒光定量核酸擴(kuò)增法對(duì)新生兒巨細(xì)胞病毒感染的診斷價(jià)值。 材料和方法： 收集淄博市婦幼保健院不明原因新生兒高膽紅素血癥標(biāo)本170例(排除高膽紅素血癥新生兒血清標(biāo)本100例為正常對(duì)照),疾病診斷符合《實(shí)用新生兒學(xué)》新生兒高膽紅素血癥診斷標(biāo)準(zhǔn),標(biāo)本分離血漿后在全自動(dòng)生化分析以上檢測(cè)肝功能,記錄肝功各項(xiàng)指標(biāo)結(jié)果,記錄膽紅素濃度；以酶聯(lián)免疫吸附法(ELISA)檢測(cè)受檢者血漿中HCMV-IgM和HCMV-IgG抗體,熒光定量PCR方法檢測(cè)血漿游離DNA濃度和單個(gè)核細(xì)胞DNA濃度,并進(jìn)行統(tǒng)計(jì)學(xué)處理。 結(jié)果： 1、新生兒高膽紅素血癥患者標(biāo)本HCMV-IgM和HCMV-IgG陽(yáng)性率均高于正常對(duì)照組,兩者比較有統(tǒng)計(jì)學(xué)意義(P0.05)。 2、單個(gè)核細(xì)胞中HCMVDNA陽(yáng)性率較血漿游離DNA高,兩者比較有統(tǒng)計(jì)學(xué)意義(P0.05)。 3、HCMV-IgG陽(yáng)性組肝功能指標(biāo)ALT、AST、GGT均高于HCMV-IgG陰性組,兩者比較有統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論： 新生兒高膽紅素血癥患者血清HCMV-IgG及HCMV-IgM陽(yáng)性率均高于正常對(duì)照組,兩者比較有統(tǒng)計(jì)學(xué)意義,說(shuō)明新生兒的巨細(xì)胞病毒感染與新生兒高膽紅素血癥及肝損傷相關(guān)性大。單個(gè)核細(xì)胞中檢測(cè)HCMV-DNA陽(yáng)性率高于血漿中的游離DNA,兩者比較有統(tǒng)計(jì)學(xué)意義,說(shuō)明在進(jìn)行HCMV的DNA檢測(cè)時(shí),宜選用單個(gè)核細(xì)胞。
[Abstract]:Objective: 1 to investigate the relationship between neonatal hyperbilirubinemia and cytomegalovirus (HCMV) infection and its clinical significance. 2. To investigate the diagnostic value of fluorescent quantitative nucleic acid amplification in neonatal cytomegalovirus infection. Materials and methods: 170 samples of neonatal hyperbilirubinemia with unknown cause were collected from Zibo Maternal and Child Health Hospital (excluding 100 serum samples of neonates with hyperbilirubinemia as normal control). The diagnosis of the disease was in accordance with the diagnostic criteria of neonatal hyperbilirubinemia. After the plasma samples were separated, the liver function was detected by automatic biochemical analysis, the results of various indexes of liver function were recorded, and the concentration of bilirubin was recorded. Enzyme linked immunosorbent assay (ELISA) was used to detect the HCMV-IgM and HCMV-IgG antibodies in the plasma of the subjects, and fluorescence quantitative PCR was used to detect the concentration of free DNA and DNA in mononuclear cells. Results: 1. The positive rates of HCMV-IgM and HCMV-IgG in neonates with hyperbilirubinemia were significantly higher than those in normal controls (P0.05). 2. The positive rate of HCMVDNA in mononuclear cells was higher than that in plasma free DNA (P0.05). 3The liver function index ALT,AST,GGT of HCMV-IgG positive group was significantly higher than that of HCMV-IgG negative group (P0.05). Conclusion: the positive rates of serum HCMV-IgG and HCMV-IgM in neonates with hyperbilirubinemia were significantly higher than those in normal controls. The results showed that neonatal cytomegalovirus infection was associated with neonatal hyperbilirubinemia and liver injury. The positive rate of HCMV-DNA in mononuclear cells was higher than that of free DNA, in plasma, which indicated that mononuclear cells should be selected for DNA detection of HCMV.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R722.1

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