【摘要】：目的：本研究試圖運(yùn)用代謝組學(xué)研究思路和技術(shù)驗(yàn)證前期研究結(jié)果對(duì)于兒童紫癜性腎炎中醫(yī)分型的合理性；明確兒童紫癜性腎炎尿液代謝物的變化特點(diǎn),探索疾病的生物標(biāo)志物；明確兒童紫癜性腎炎熱傷腎絡(luò)證尿液代謝物的變化特點(diǎn),探索證候的生物標(biāo)志物；從代謝組學(xué)水平探討兒童紫癜性腎炎中醫(yī)證型的物質(zhì)基礎(chǔ)及診斷標(biāo)準(zhǔn)。方法：采集符合納入標(biāo)準(zhǔn)的兒童紫癜性腎炎熱傷腎絡(luò)證及非熱傷腎絡(luò)證患兒各9例,及健康體檢者22例的空腹晨尿中段尿,-80℃冰箱存儲(chǔ),樣本采集齊后利用超高液相色譜-離子肼質(zhì)譜系統(tǒng)進(jìn)行譜圖掃描,取得各樣品的代謝指紋譜,經(jīng)譜圖預(yù)處理,得出具有保留時(shí)間、質(zhì)荷比、峰面積的三維數(shù)據(jù),利用多元統(tǒng)計(jì)分析方法中的主成分分析考察樣本分布總體情況,進(jìn)一步使用偏最小二乘法及經(jīng)正交校正的偏最小二乘法對(duì)各組的代謝指紋譜進(jìn)行比較,根據(jù)載荷圖,以及主成分貢獻(xiàn)值及T檢驗(yàn),得出區(qū)別各組間的重要化合物,這些化合物可能是疾病和證型的“生物標(biāo)記物群”結(jié)果：(1)兒童紫癜性腎炎和正常對(duì)照之間可以明顯區(qū)分。(2)兒童紫癜性腎炎熱傷腎絡(luò)證和正常對(duì)照組可以明顯區(qū)分。(3)兒童紫癜性腎炎非熱傷腎絡(luò)證組樣本分布較散亂。(4)根據(jù)數(shù)據(jù)庫檢索,區(qū)別兒童紫癜性腎炎的化合物可能是羥基絡(luò)醇4-硫酸酯、精氨酰絲氨酸、雌酮、色氨酰蛋氨酸、維生素K2、鈍葉素葡萄糖苷、S-氨甲基二氫硫、苯丙精氨酸、L-蛋氨酸、同型半胱氨酸硫內(nèi)酯、膽甾-3,7,12,25四醇-3-葡糖苷酸、磷脂酰膽堿、乙酰乙酸、N2,N2-二甲基鳥苷；區(qū)別兒童紫癜性腎炎熱傷腎絡(luò)證的化合物可能是丙酸、醛固酮、均聚L-精氨酸、維生素K2、鈍葉素葡萄糖苷、S-氨甲基二氫硫、苯丙精氨酸、L-蛋氨酸、同型半胱氨酸硫內(nèi)酯、羥基絡(luò)醇4-硫酸酯、精氨酰絲氨、雌酮、色氨酰蛋氨。結(jié)論：(1)代謝組學(xué)的研究方法可以成功區(qū)分兒童紫癜性腎炎和正常對(duì)照組,可能的差異化合物有羥基絡(luò)醇4-硫酸酯、精氨酰絲氨酸、雌酮、色氨酰蛋氨酸、維生素K2、鈍葉素葡萄糖苷、S-氨甲基二氫硫、苯丙精氨酸、L-蛋氨酸、同型半胱氨酸硫內(nèi)酯、膽甾-3,7,12,25四醇-3-葡糖苷酸、磷脂酰膽堿、乙酰乙酸、N2,N2-二甲基鳥苷。(2)代謝組學(xué)的研究方法可以成功區(qū)分兒童紫癜性腎炎熱傷腎絡(luò)證和正常對(duì)照組,可能的差異性化合物是丙酸、醛固酮、均聚L-精氨酸、維生素K2、鈍葉素葡萄糖苷、S-氨甲基二氫硫、苯丙精氨酸、L-蛋氨酸、同型半胱氨酸硫內(nèi)酯、羥基絡(luò)醇4-硫酸酯、精氨酰絲氨酸、雌酮、色氨酰蛋氨酸。(3)兒童紫癜性腎炎熱傷腎絡(luò)證其代謝表征的確發(fā)生了變化,前期兒童紫癜性腎炎中醫(yī)證型的研究結(jié)果中對(duì)于熱傷腎絡(luò)證證型的劃分是有物質(zhì)基礎(chǔ)和科學(xué)性的。
[Abstract]:Objective: this study attempts to verify the rationality of the results of previous studies on TCM classification of Henoch-Schonlein purpura nephritis (HSPN) in children by means of metabonomics research ideas and techniques. The changes of urine metabolites in children with Henoch-Schonlein purpura nephritis (HSPN) and the biomarkers of the disease were investigated, and the changes of urinary metabolites in children with Henoch-Schonlein purpura nephritis (HSPN) were determined. To discuss the material basis and diagnostic criteria of TCM syndromes of Henoch-Schonlein purpura nephritis in children from the level of metabonomics. Methods: according to the inclusive criteria, 9 cases of febrile injury of kidney collateral syndrome and 9 cases of non-heat injury syndrome of kidney collateral syndrome in children with Henoch-Schonlein purpura nephritis were collected, and 22 cases of healthy check-up were collected from fasting morning urine and stored in -80 鈩，

本文編號(hào)：2331218