本文選題：蛋白質(zhì)精氨酸甲基轉(zhuǎn)移酶1 + 先天性巨結(jié)腸��； 參考：《青島大學(xué)》2012年碩士論文

【摘要】：目的：蛋白質(zhì)精氨酸甲基轉(zhuǎn)移酶1(PRMT1)在細(xì)胞中精氨酸的甲基化過程中起到重要作用,在神經(jīng)元細(xì)胞的生長和發(fā)育過程中有著重要意義。先天性巨結(jié)腸(HD)的病理特點(diǎn)為末端腸段神經(jīng)節(jié)細(xì)胞的缺乏。在本研究中,將檢測PRMT1在HD患兒結(jié)腸狹窄段(HD組)及正常結(jié)腸組織(對照組)中的表達(dá)情況,探討HD發(fā)病機(jī)制。 方法：標(biāo)本取自2010年9月至2011年10月間行手術(shù)治療患兒,20例HD組標(biāo)本取自狹窄腸段(無神經(jīng)節(jié)細(xì)胞腸段),20例對照組標(biāo)本取自正常腸段組織。40例患兒均在聊城市人民醫(yī)院和聊城市第二人民醫(yī)院行手術(shù)治療。手術(shù)中采取HD患兒痙攣段及對照組患兒新鮮結(jié)腸標(biāo)本,立即送至實(shí)驗(yàn)室,置于-86℃低溫冰箱內(nèi)保存,所有標(biāo)本收集后進(jìn)行檢測。通過Western Blot實(shí)驗(yàn)分析腸段PRMT1蛋白表達(dá)量；通過免疫組織化學(xué)實(shí)驗(yàn)觀察PRMT1在結(jié)腸各層組織細(xì)胞中的表達(dá)情況。 結(jié)果：HD組狹窄段腸段PRMT1蛋白含量明顯減少(p0.05)。兩組腸粘膜隱窩細(xì)胞PRMT1均顯著表達(dá)。在對照組粘膜下層與肌間神經(jīng)叢PRMT1顯著表達(dá),HD組沒有PRMT1的明顯表達(dá)(p0.05),這正是HD患兒的特征。 結(jié)論：結(jié)腸組織PRMT1表達(dá)量減少可能導(dǎo)致HD發(fā)生；PRMT1作為一種標(biāo)志物來診斷HD有一定價(jià)值。
[Abstract]:Aim: protein arginine methyltransferase 1 (PRMT1) plays an important role in the methylation of arginine in cells and plays an important role in the growth and development of neuronal cells. The histopathological feature of HD in Hirschsprung's disease is the lack of ganglion cells in the terminal segment of the intestine. In this study, the expression of PRMT1 was detected in HD patients with colon stenosis and normal colon tissues (control group) to investigate the pathogenesis of HD. Methods: 20 patients with HD received surgical treatment from September 2010 to October 2011 were collected from narrow intestinal segment (20 cases without ganglion cell segment of intestine) and 20 cases from normal intestinal segment tissue. 40 cases of children in Liaocheng City were enrolled in this study. People's Hospital and Liaocheng second people's Hospital for surgical treatment. The spastic sections of HD children and the fresh colon samples of the control group were sent to the laboratory immediately and stored in the cryogenic refrigerator at -86 鈩，

本文編號：1928690