本文選題：鮑曼不動(dòng)桿菌 + 兒科患者�。� 參考：《中國抗生素雜志》2009年03期

【摘要】：目的研究從兒科肺炎患者中分離的鮑曼不動(dòng)桿菌對(duì)氨基糖苷類抗生素的耐藥性和常見的7種氨基糖苷類修飾酶基因特征。方法56株鮑曼不動(dòng)桿菌(AB)收集自2006年分離的兒科臨床肺炎患兒的深部痰培養(yǎng)標(biāo)本,均采用VITEK-32全自動(dòng)微生物鑒定儀GNI和GNS卡進(jìn)行細(xì)菌鑒定和藥敏試驗(yàn)。氨基糖苷類修飾酶基因檢測采用聚合酶鏈?zhǔn)椒磻?yīng)(PCR)方法。結(jié)果檢測的56株鮑曼不動(dòng)桿菌菌有8株呈多重耐藥性,陽性率14.29%,8株多重耐藥菌對(duì)氨基糖苷類藥物阿米卡星、妥布霉素和慶大霉素均耐藥,其余菌株對(duì)氨基糖苷類藥物均敏感,氨基糖苷類藥物的耐藥率14.29%。除呋喃妥因及β-內(nèi)酰胺類抗生素氨芐西林、頭孢唑林和頭孢曲松外其它抗生素的耐藥率均在15%以下。7種氨基糖苷類修飾酶基因中檢出2株aac(3)-Ⅱ(3.57%),2株aac(6')-Ⅰb(3.57%),4株aac(6')-Ⅱ(7.14%),6株ant(3″)-Ⅰ(10.71%);aac(3)-Ⅰ、ant(2″)-Ⅰ和aac(6')-Ⅰad均陰性。對(duì)3種氨基糖苷類抗生素耐藥的菌株均檢出了氨基糖苷類修飾酶基因。結(jié)論(1)兒科患者雖然極少應(yīng)用氨基糖苷類抗生素,但由于氨基糖苷類修飾酶基因可借助于整合子、轉(zhuǎn)座子和質(zhì)粒等在同種和異種細(xì)菌間傳播,使其對(duì)氨基糖苷類抗生素也產(chǎn)生了一定的耐藥性。(2)鮑曼不動(dòng)桿菌兒科患者分離株對(duì)阿米卡星、慶大霉素和妥布霉素的耐藥與aac(3)-Ⅱ、aac(6')-Ⅰb、aac(6')-Ⅱ和ant(3″)-Ⅰ四種基因有關(guān)。不同地區(qū)細(xì)菌的修飾酶基因有很大差異,而兒童與成人患者亦有不同,因此要重視兒科患者鮑曼不動(dòng)桿菌氨基糖苷類抗生素耐藥性和耐藥基因研究。
[Abstract]:Objective to study the antimicrobial resistance of Acinetobacter baumannii to aminoglycoside antibiotics and the characteristics of seven common aminoglycoside modified enzyme genes in pediatric patients with pneumonia. Methods 56 strains of Acinetobacter baumannii were collected from pediatric children with clinical pneumonia in 2006. The bacteria were identified by VITEK-32 GNI and GNS card. Aminoglycoside modified enzyme gene was detected by polymerase chain reaction (PCR). Results among 56 strains of Acinetobacter baumannii, 8 strains showed multidrug resistance. The positive rate was 14.29% and 8 strains were resistant to aminoglycoside drugs such as amikacin, tobramycin and gentamicin. The other strains were sensitive to aminoglycosides. The resistance rate of aminoglycosides was 14.29%. Defurantoin and 尾-lactam antibiotics ampicillin, The resistance rates of cefazolin and ceftriaxone to other antibiotics were below 15%. Two of the 7 aminoglycoside modified enzyme genes were found to be negative in two strains of aacantoin (3. 57)-1. 3. 57 and 4 strains of aacanthus 6-2. 7. 14. 14. 6 strains of ant(3 "- 鈪，

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