本文選題：IL- + 金黃色葡萄球菌�。� 參考：《中國當(dāng)代兒科雜志》2017年06期

【摘要】：目的觀察重組融合蛋白IL-18對金黃色葡萄球菌(SA)感染后小鼠體內(nèi)免疫相關(guān)炎癥因子表達的影響,探討IL-18在體內(nèi)防御SA感染的可能機制。方法將40只SPF級雌性BLAB/c小鼠隨機分為對照組、SA感染組、免疫組和干預(yù)組。采用鼻腔接種SA液建立SA感染小鼠模型,免疫組和干預(yù)組均在建模前以IL-18滴鼻,但免疫組不予SA接種,對照組以PBS進行替代處理。采用ELISA法測定各組小鼠血液及支氣管肺泡灌洗液(BALF)中IL-4、干擾素-γ(IFN-γ)、腫瘤壞死因子(TNF)、粒細胞集落刺激因子(G-CSF)、Ig M的濃度。實時熒光定量PCR技術(shù)檢測各組小鼠肺組織中巨噬細胞炎性蛋白(MIP)-1α和MIP-2βm RNA表達水平。結(jié)果與對照組相比,SA感染組和免疫組小鼠血清及BALF中IL-4、G-CSF、Ig M濃度,以及肺組織中MIP-1α、MIP-2βm RNA含量均升高(P0.05);SA感染組小鼠血清及BALF中IFN-γ水平降低,TNF水平升高(P0.05);免疫組小鼠血清及BALF中IFN-γ水平升高(P0.05)。與SA感染組相比,干預(yù)組小鼠血清及BALF中IL-4、IFN-γ、G-CSF、Ig M濃度,以及肺組織中MIP-1αm RNA含量均升高,血清及BALF中TNF水平,以及肺組織中MIP-2βm RNA含量均降低(P0.05)。除血清IFN-γ水平外,其余上述指標(biāo)在干預(yù)組小鼠中均高于對照組(P0.05)。結(jié)論重組融合蛋白IL-18經(jīng)黏膜免疫小鼠,可改變SA感染后小鼠血清及BALF中炎癥因子,以及肺組織中MIP-1α、MIP-2βm RNA的表達水平,從而促進機體的抗感染免疫反應(yīng),增強了機體清除病原體的能力。
[Abstract]:Objective to observe the effect of recombinant fusion protein IL-18 on the expression of immune-associated inflammatory factors in mice infected with staphylococcus aureus and to explore the possible mechanism of IL-18 in the defense of SA infection in vivo. Methods 40 SPF female BLAB/c mice were randomly divided into three groups: control group, immune group and intervention group. The model of SA-infected mice was established by nasal inoculation with SA solution. Both the immune group and the intervention group were given IL-18 nasal drip before modeling, but the immune group was not inoculated with SA, and the control group was treated with PBS. The concentrations of IL-4, IFN- 緯, TNFF and G-CSF- IFN- 緯 in blood and bronchoalveolar lavage fluid (BALF) of mice in each group were determined by ELISA method. The expression of macrophage inflammatory protein (MIP-1 偽) and MIP-2 尾 m RNA in lung tissue of mice in each group was detected by real-time fluorescence quantitative PCR. Results compared with the control group, the serum and BALF levels of IL-4G-CSFG in the SA infected and immunized mice were higher than those in the control group. The levels of IFN- 緯 in serum and BALF of mice infected with P0.05 were increased, and the levels of IFN- 緯 in serum and BALF of immunized mice were increased. Compared with SA infection group, the concentration of IL-4 IFN- 緯 G-CSFFG G M in serum and BALF, the content of MIP-1 偽 m RNA in lung tissue, the TNF level in serum and BALF, and the content of MIP-2 尾 m RNA in lung tissue in the intervention group were all lower than those in the control group (P 0.05). With the exception of serum IFN- 緯, the above indexes in the intervention group were higher than those in the control group (P 0.05). Conclusion the recombinant fusion protein IL-18 can change the levels of inflammatory factors in serum and BALF and the expression level of MIP-1 偽 -MIP-2 尾 m RNA in lung tissue of SA-infected mice, thus promoting the anti-infection immune response. Enhanced the body's ability to remove pathogens.
【作者單位】： 江西省兒童醫(yī)院呼吸內(nèi)科;
【分類號】：R725.1
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本文編號：1830081