發(fā)布時間：2018-04-17 15:13

  本文選題：槐杞黃顆粒 + 阿霉素腎病��； 參考：《重慶醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的：觀察炎癥因子白介素-17（interleukin-17）在阿霉素腎病小鼠腎小球硬化進程中表達情況，探討槐杞黃顆粒對小鼠阿霉素腎病的保護作用及可能機制。 方法：雄性，8w，BAL b/c小鼠隨機分為正常對照組、阿霉素腎病組和阿霉素腎病阿霉素腎病槐杞黃治療組，每組12只。經(jīng)尾靜脈一次性注射10mg/kg阿霉素建立阿霉素腎病小鼠模型。阿霉素腎病槐杞黃治療組自阿霉素注射后第7d起，每日給予4g/kg槐杞黃清膏灌胃，正常對照組和阿霉素腎病組每日給予等量蒸餾水灌胃，灌胃共7周。觀察小鼠一般狀況和體重改變。阿霉素注射后第2、8周末分別處死小鼠，每組各6只。光學(xué)顯微鏡下觀察腎組織病理改變；免疫組化檢測腎臟局部IL-17表達情況；考馬斯亮藍法檢測24h尿蛋白含量；全自動生化分析儀檢測血清白蛋白和肌酐水平。 結(jié)果：實驗前正常對照組、阿霉素腎病組、阿霉素腎病槐杞黃治療組小鼠體重和24h尿蛋白含量無明顯差別。阿霉素腎病組小鼠自實 驗第2周開始出現(xiàn)大量蛋白尿、血清白蛋白降低持續(xù)至實驗結(jié)束（與正常對照組比較，P0.05）；實驗第8周，腎組織IL-17表達明顯增強，伴有明顯的局灶節(jié)段性腎小球硬化，甚至腎小球硬化及腎功能不全（與正常對照組比較，P0.05）。與同時間點阿霉素腎病組相比，阿霉素腎病槐杞黃治療組小鼠24h尿蛋白明顯降低、血清白蛋白升高、腎組織病理改變及腎功能損害程度減輕（P0.05）。 結(jié)論：槐杞黃可能通過降低阿霉素腎病小鼠腎組織IL-17表達，減輕腎臟局部炎癥反應(yīng)，發(fā)揮腎臟起保護作用。 目的：探討interleukin-17(IL-17)對人系膜細胞體外增殖、合成分泌細胞外基質(zhì)的影響及槐耳清膏的干預(yù)作用。 方法：體外培養(yǎng)的人系膜細胞分為正常對照組、 IL-17組、IL-17+槐耳清膏組、槐耳清膏對照組。臺盼藍拒染法檢測槐耳清膏的細胞毒性，MTT法測系膜細胞增殖情況，ELISA檢測細胞培養(yǎng)上清液中纖連蛋白（FN）水平。 結(jié)果：（1）各實驗濃度槐耳清膏均無明顯細胞毒性作用；（2）與正常對照組比較，不同濃度IL-17均可促進系膜細胞增殖（P0.05），各濃度之間無明顯差異；不同濃度的槐耳清膏可抑制IL-17誘導(dǎo)的系膜細胞增殖，與IL-17（10ng/ml）組比較有顯著差異（P0.05）；（3）槐耳清膏可抑制IL-17促系膜細胞合成分泌FN的作用，且可能具有一定時間量效關(guān)系。 結(jié)論: IL-17能夠誘導(dǎo)人系膜細胞增殖，促進系膜細胞合成分泌纖連蛋白。槐耳清膏可能通過抑制IL-17的刺激作用，發(fā)揮腎臟保護功能，從而延緩腎小球硬化的進展。
[Abstract]:Objective: To observe the expression of inflammatory factor interleukin -17 (interleukin-17) in the glomerulosclerosis process of adriamycin nephropathy mice, and to explore the protective effect and possible mechanism of Huai Qi Huang Granule on adriamycin nephrosis in mice.
Methods: male, 8W, BAL b/c mice were randomly divided into normal control group, adriamycin nephropathy group and adriamycin adriamycin nephropathy Huaiqihuang treatment group, 12 rats in each group. Adriamycin nephropathy mouse model by tail vein injection of 10mg/kg adriamycin. Adriamycin nephropathy Huaiqihuang treatment group after adriamycin injection 7d, daily give 4g/kg Huaiqihuang creamwith gavage, the normal control group and adriamycin group daily given distilled water orally by gavage for 7 weeks. The general condition and body weight of mice was observed. After adriamycin injection the mice were sacrificed after 2,8 weeks, 6 rats in each group. The renal pathologic changes were observed under light microscope; immune immunohistochemical detection of IL-17 in kidney of expression; detection of 24h urinary protein content by Coomassie brilliant blue method; automatic biochemical analyzer to detect the serum albumin and creatinine levels.
Results: there was no significant difference in body weight and 24h urine protein between the normal control group, adriamycin nephrosis group, adriamycin nephrosis group and Huai Qi Huang group before treatment.
Test second weeks massive proteinuria, serum albumin decreased continuously until the end of the experiment (compared with normal control group, P0.05); the eighth week, the expression of IL-17 was significantly enhanced, focal segmental glomerular sclerosis accompanied by obvious, even glomerular sclerosis and renal insufficiency (compared with normal control group, P0.05). Compared with the same time point of adriamycin nephropathy group, adriamycin nephropathy Huaiqihuang treatment group were significantly lower 24h urinary protein, serum albumin increased, the pathological change of kidney tissue and renal function damage reduction (P0.05).
Conclusion: sophorum huyfcii may reduce the expression of IL-17 in renal tissue of adriamycin nephrotic mice, reduce the local inflammatory reaction of kidney and play the protective role of kidney.
Objective: To investigate the effect of interleukin-17 (IL-17) on the proliferation of mesangial cells in vitro, affect the synthesis and secretion of extracellular matrix and effects of PS-T.
Methods: in vitro cultured human mesangial cells were divided into normal control group, IL-17 group, IL-17+ PS-T group, PS-T control group. Trypan blue to detect cytotoxicity of PS-T staining method, measuring mesangial cell proliferation by MTT method, the supernatant of fibronectin in cultured ELISA cells (detection the level of FN).
Results: (1) the experimental concentration of PS-T had no obvious cytotoxic effect; (2) compared with the normal control group, different concentration of IL-17 can promote the proliferation of mesangial cells (P0.05), no significant difference between the concentration; different concentrations of Huaier cream can inhibit the proliferation of mesangial cells induced by IL-17, and IL-17 (10ng/ml) group had significant difference (P0.05); (3) PS-T inhibited IL-17 induced mesangial cell synthesis and secretion of FN, and may have a certain amount of time effect relationship.
Conclusion: IL-17 can induce the proliferation of mesangial cells, promote mesangial cell synthesis and secretion of fibronectin. PS-T through inhibition of IL-17 stimulation, to prevent renal function, thereby delaying the progression of glomerular sclerosis.

【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R285.5;R726.9

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