發(fā)布時間：2018-04-04 09:23

  本文選題：魚油　切入點：膿毒癥　出處：《南昌大學(xué)》2017年碩士論文

【摘要】：目的:研究膿毒癥幼鼠腸道菌群紊亂、易位和血清炎癥因子TNF-α的變化,以及魚油干預(yù)后對上述情況的影響。方法:1、實驗分組及喂養(yǎng)方式:選取24只健康SD幼鼠(4周齡),根據(jù)完全隨機(jī)分組原則分為三組:正常對照組,模型組及魚油治療組(每組各8只)。正常組為正常狀態(tài)每日飼喂基礎(chǔ)日糧,模型組飼喂每日基礎(chǔ)日糧,魚油治療組則是基礎(chǔ)日糧加魚油灌胃。按上述方式分別喂養(yǎng)1周。第8天時模型組、魚油治療組幼鼠皆制作膿毒癥模型,并依照5mg/kg的劑量經(jīng)腹腔注射制劑——大腸桿菌脂多糖(LPS)的方法造模,三組繼續(xù)如上述喂養(yǎng)方式喂養(yǎng)3d。2、標(biāo)本采集:制模第三天下午2時開胸收集實驗所需各組織,心臟取血用于ELISA實驗測定血清TNF-α,采集并準(zhǔn)確稱量肝、脾、腎、腸系膜淋巴結(jié)、結(jié)腸內(nèi)容物各1g,在距離屈氏韌帶8厘米處迅速切除約10厘米回腸段置于福爾馬林中浸泡以用于病理學(xué)檢查。3、觀察指標(biāo):觀察各組幼鼠腸道菌群(雙歧桿菌、乳酸桿菌、大腸桿菌、葡萄球菌)及細(xì)菌易位率的變化及比較;ELISA實驗測定各組幼鼠血清炎癥因子TNF-α數(shù)值變化;采用蘇木精—伊紅染色法(HE染色),顯微鏡下取每組(各組隨機(jī)2只)幼鼠回腸組織觀察病理學(xué)改變。結(jié)果:1、一般表現(xiàn):模型組制模后出現(xiàn)病態(tài)表現(xiàn):精神差,活動、食納減少,寒戰(zhàn)豎毛,呼吸增快,口唇發(fā)紺。剖腹時可見少數(shù)幼鼠存在腹腔積液,部分腸管腫脹、發(fā)黑。魚油治療組與模型組對比,各病態(tài)表現(xiàn)均有所減輕,正常對照組無上述病態(tài)表現(xiàn)。2、膿毒癥幼鼠存在腸道菌群紊亂狀態(tài),在細(xì)菌具體數(shù)量上表現(xiàn)為腸道雙歧桿菌及腸道乳酸桿菌明顯下降(P0.01),腸道大腸桿菌及葡萄球菌則明顯升高(P0.01),魚油對膿毒癥幼鼠菌群紊亂具有一定糾正作用(P0.01)。它增加了上述腸道雙歧、乳酸桿菌的數(shù)目(P0.01),降低了大腸桿菌、葡萄球菌的數(shù)目(P0.01),減少了腸道細(xì)菌易位的發(fā)生。3、膿毒癥幼鼠血清炎癥因子TNF-α明顯升高(P0.01),魚油能顯著減低TNF-α數(shù)值(P0.01)。4、膿毒癥幼鼠存在腸道粘膜組織學(xué)損傷,魚油可改善膿毒癥幼鼠回腸黏膜損傷和回腸組織病理變化。結(jié)論:膿毒癥幼鼠腸道菌群存在紊亂及易位,伴腸道粘膜損傷,血清炎癥因子TNF-α明顯升高;魚油干預(yù)可促進(jìn)腸道雙歧、乳酸桿菌的生長使數(shù)量增加,抑制大腸桿菌及葡萄球菌而使數(shù)量降低;可減少細(xì)菌易位的發(fā)生;對血清TNF-α的釋放具有一定抑制作用,可減輕組織和器官的一系列損傷;改善了膿毒癥幼鼠回腸黏膜損傷和回腸組織病理變化,使腸道免疫屏障保持完整,提高腸道黏膜修復(fù)力。
[Abstract]:Aim: to study the changes of intestinal flora disturbance, translocation and serum inflammatory factor TNF- 偽 in septic young rats.Methods: 24 healthy SD rats were randomly divided into three groups: normal control group, model group and fish oil treatment group (8 rats in each group).The normal group was fed the basic diet daily, the model group was fed the daily basic diet, and the fish oil treatment group was fed with the basic diet plus fish oil.The animals were fed for 1 week according to the above method.On the 8th day, the model group, fish oil treatment group all made sepsis model, and according to the dose of 5mg/kg through intraperitoneal injection preparation-Escherichia coli lipopolysaccharide lipopolysaccharide (LPS), the model was made.The three groups continued to feed 3d.2as mentioned above. Specimen collection: the tissues needed for the open-chest collection experiment were collected at 2: 00 p.m. on the third day of model making. Blood was taken from the heart to determine serum TNF- 偽 by ELISA test. The liver, spleen, kidney and mesenteric lymph nodes were collected and weighed accurately, and the liver, spleen, kidney and mesenteric lymph nodes were measured accurately.Colon contents, 1 g each, were quickly removed about 10 cm from the tricuspid ligament and immersed in formalin for pathological examination .3.The indicators: the intestinal flora (Bifidobacterium, Lactobacillus) was observed in each group.Comparison of the changes of Escherichia coli, Staphylococcus) and bacterial translocation rate in young rats serum TNF- 偽 was determined by Elisa.Hematoxylin-eosin staining was used to observe the pathological changes in ileum of each group (2 randomly in each group).Results: in the model group, there were pathological manifestations after modeling: mental retardation, activity, decrease of food intake, shivering hair, rapid breathing, cyanosis of lips.During laparotomy, a few young rats were found to have abdominal effusion, swelling and blackening of the intestine.Compared with the model group, the pathological manifestations of fish oil treatment group were alleviated, and the normal control group did not have the pathological manifestation. 2. The septic young rats had intestinal flora disorder state.The specific number of bacteria showed that intestinal bifidobacterium and intestinal Lactobacillus decreased significantly (P 0.01), intestinal Escherichia coli and Staphylococcus significantly increased P 0.01, fish oil had a certain corrective effect on sepsis young rat flora disorder (P0.01).It increases the number of Lactobacillus bifidus, P0. 01, and reduces the number of Escherichia coli.The number of staphylococci decreased the occurrence of intestinal bacterial translocation. The inflammatory factor TNF- 偽 in serum of septic young rats was significantly increased. Fish oil could significantly reduce the value of TNF- 偽, P0.01. 4. There were intestinal mucosal tissue damage in sepsis young rats.Fish oil can improve ileal mucosal injury and ileal histopathological changes in septic young rats.Conclusion: the intestinal flora of sepsis young rats has disorder and translocation, accompanied by intestinal mucosal injury, serum inflammatory factor TNF- 偽 is obviously increased, fish oil intervention can promote intestinal bifidus, Lactobacillus growth can increase the number.Inhibition of Escherichia coli and Staphylococcus can reduce the number of bacteria translocation can reduce the release of serum TNF- 偽 can reduce a series of tissue and organ damage.It improved the ileal mucosal injury and the pathological changes of ileum in septic young rats, kept the intestinal immune barrier intact and improved the repair ability of intestinal mucosa.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R720.597

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