本文選題：高脂飲食　切入點(diǎn)：氣道　出處：《中國(guó)當(dāng)代兒科雜志》2017年07期

【摘要】：目的探討高脂飲食對(duì)瞬時(shí)感受器電位陽離子通道香草酸-1(TRPV1)在小鼠呼吸系統(tǒng)和背根神經(jīng)節(jié)(DRG)上的表達(dá)變化,并研究其對(duì)感覺神經(jīng)元興奮性的影響。方法將C57BL/6小鼠20只隨機(jī)分成普通飼料組和高脂飼料組,每組10只。分別喂養(yǎng)相應(yīng)飼料并監(jiān)測(cè)體重。喂養(yǎng)7周后取兩組小鼠肺葉、支氣管組織,以及胸3~4段DRG行免疫組化染色檢測(cè)TRPV1表達(dá)水平,并用膜片鉗測(cè)量DRG動(dòng)作電位和TRPV1電流。結(jié)果經(jīng)7周飼養(yǎng)后,高脂飼料組平均增重(6.4±2.6 g)顯著大于普通飼料組(2.3±0.5 g)(P0.001)。高脂飼料組支氣管、肺泡和DRG上TRPV1的陽性表達(dá)均明顯高于普通飼料組(P0.05)。高脂飼料組DRG上TRPV1電流強(qiáng)度和動(dòng)作電位激發(fā)數(shù)目較普通飼料組均明顯升高(P0.05)。結(jié)論高脂飲食引起小鼠體重明顯增加,導(dǎo)致小鼠呼吸系統(tǒng)和外周感覺神經(jīng)元中TRPV1呈現(xiàn)高表達(dá)和易激惹高興奮性,提示TRPV1可能是導(dǎo)致支氣管超高反應(yīng)病理生理機(jī)制中的一個(gè)重要因子。
[Abstract]:Objective to investigate the effect of high fat diet on the expression of vanillic acid-1 (vanillic acid-1) in the respiratory system and dorsal root ganglion (DRG) of mice and its effect on the excitability of sensory neurons.Methods Twenty C57BL/6 mice were randomly divided into normal diet group and high fat diet group with 10 mice in each group.The corresponding feed was fed and the body weight was monitored.After feeding for 7 weeks, the expression of TRPV1 was detected by immunohistochemical staining in lung lobes, bronchi and 4 segments of thoracic DRG of mice. DRG action potential and TRPV1 current were measured with patch clamp.Results after feeding for 7 weeks, the average weight gain of the high-fat diet group was 6.4 鹵2.6 g, which was significantly higher than that of the normal diet group (2.3 鹵0.5 g / kg, P 0.001).The positive expression of TRPV1 in bronchi, alveoli and DRG in high fat diet group was significantly higher than that in normal diet group.The number of TRPV1 current and action potential excitation on DRG in high fat diet group was significantly higher than that in normal diet group.Conclusion High fat diet can significantly increase the body weight of mice and induce high expression of TRPV1 in respiratory system and peripheral sensory neurons of mice.It suggests that TRPV1 may be an important factor in the pathophysiological mechanism of bronchial hyperthermia.
【作者單位】： 武漢大學(xué)人民醫(yī)院兒科;孝感市婦幼保健院兒科;
【分類號(hào)】：R725.6

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