盡管支原體缺乏細(xì)胞壁,而且只有很小的基因組,但各屬的支原體都能夠引起不同宿舍的慢性感染。豬鼻支原體的較高感染性可能是由于豬的生長(zhǎng)環(huán)境發(fā)生了變化,而更大程度上因?yàn)樨i肉生產(chǎn)系統(tǒng)向?qū)I(yè)化,集約化演變而導(dǎo)致。然而,豬鼻支原體是通過鼻子對(duì)鼻子來傳播,通過感染豬的接觸或谷倉、農(nóng)場(chǎng)中的空氣傳播等。雖然M.豬鼻是豬的上呼吸道中正常菌群的一部分,但它具有致病性。它是關(guān)節(jié)炎,胸膜炎,心包炎和腹膜炎的病原體,并容易引起乳豬的地方性肺炎。大多數(shù)感染在亞臨床和臨床癥狀表現(xiàn)上可能有所不同,而且混合感染也很常見。菌株毒力的變化,天氣的變化,還有病原體和宿主的免疫系統(tǒng)等這些機(jī)制都是系統(tǒng)性的。在基因組水平,有報(bào)道稱不同牛群中分離出來的具有極大的異質(zhì)性。在一個(gè)農(nóng)場(chǎng)中的同一動(dòng)物中分離出來的不同菌株,可能在毒力或引起干擾和交叉反應(yīng)方面有所不同。在新陳代謝中,豬鼻支原體基因中的一些變化可以逃避宿主的免疫系統(tǒng),這可能有助于其感染。目前的研究主要集中在從不同方面來控制豬鼻支原體。（1）豬病原體的檢測(cè)主要是豬鼻支原體和豬肺炎支原體。臨床樣品為7-35天的豬鼻拭子,主要是來自中國的江蘇省,他們從不同患病牛群中收集了病原體,利用巢式PC... 

【文章來源】：南京農(nóng)業(yè)大學(xué)江蘇省 211工程院校 教育部直屬院校

【文章頁數(shù)】：119 頁

【學(xué)位級(jí)別】：碩士

【文章目錄】：
摘要
ABSTRACT
CHAPTER 1 LITERATURE REVIEW
    1.1. TAXONOMY AND A GENERAL INTRODUCTION TO MYCOPLASMAS
    1.2. ECOLOGY AND PATHOGENESIS OF MYCOPLASMA
        1.2.1 Evasion of the Immune system
        1.2.2 Interaction with the Host Cells and Cytopathology
        1.2.3 M.hyorhinis Major Immunogen,P37
    1.3. ETIOLOGYY
    1.4. EPIDEMIOLOGY
    1.5. CLINICAL PRESENTATION OF MHR INFECTION IN SWINE
    1.6. DIAGNOSTIC TECHNIQUES FOR THE DETECTION OF MHR
        1.6.1 Diagnosis
        1.6.2 Macroscopic and microscopic examination
        1.6.3 Fluorescent antibody and immunohistochemistry assays
        1.6.4 Culture
        1.6.5 Serology
        1.6.6 Polymerase chain reaction(PCR)
    1.7. BIOSECURITY AND AN INTEGRATED APPROACH FOR THE MANAGEMENT OF MHR
    1.8. THE SWINE INDUSTRY IN KENYAAND CHINA
    1.9. PROBLEM STATEMENT
    1.10 RATIONALE
    1.11 HYPOTHESIS
    1.12 OBJECTIVES OF THE STUDY
CHAPTER 2 DETECTION OF RESPIRATORY PATHOGENS M.hyorhinis AND M.hyopneumoniae BY NESTEDPCR IN PORCINE NASAL SWABS FROM CLINICALLY INFECTED HERDS
    2.1 INTRODUCTION
    2.2 MATERIALS AND METHODS
        2.2.1 Animals
        2.2.2 Sample preparation
        2.2.3 Nested PCR
        2.2.4 Statistical analysis
    2.3 RESULTS
    2.4 DISCUSSION
CHAPTER 3 DETECTION,ISOLATION AND IDENTIFICATION OF M.hyorhinis AND ASSESSING THEANTIMICROBIAL PROFILES OF THE ISOLATES
    3.1 DETECTION,ISOLATION AND IDENTIFICATION OF MHR
        3.1.1 Introduction
        3.1.2 Materials and Methods
        3.1.3 Results
    3.2 ISOLATION OF MHR STRAINS
        3.2.1 Introduction
        3.2.2 Materials and Methods
        3.2.3 Results
        3.2.4 Discussion
    3.3. THE IN VITRO ASSESSMENT OF THE ANTIMICROBIAL PROFILES OF 8 MHR ISOLATES AGAINST
        3.3.1 Introduction
        3.3.2 Materials and Methods
        3.3.3 Results
        3.3.4 Discussion
CHAPTER 4 IMMUNOGENICITY TESTING IN MICE AND DETERMINATION OF ANTIBODY TITERS BYINDIRECT-ELISA
    4.1 INTRODUCTION
        4.1.1 Freund's adjuvant
        4.1.2 Immunoglobulins
    4.2 MATERIALS AND METHODS
        4.2.1 Antigen Preparation
        4.2.2 Quantification of Antigen by CCU
        4.2.3 Quantification of Antigen by BCA
        4.2.4 Quantification of Antigen using qPCR
        4.2.5 Results
        4.2.6 Immunization
        4.2.7 Detection of serum IgG antibody
            4.2.7.1 Preparation of coating protein
            4.2.7.2 Indirect IgG ELISA Assay(Tween-20 ELISA)
        4.2.8 Statistical Analysis
    4.3 RESULTS
    4.4 DISCUSSION
CHAPTER 5 PRIMARY STUDY OF INFECTION AND DEVELOPMENT OF A CHALLENGE MODEL FOR M.hyorhinis-INDUCED PATHOGENESIS IN SWINE
    5.1 INTRODUCTION
    5.2 MATERIALS AND METHODS
        5.2.1 Animals
        5.2.2 Experiment 1
        5.2.3 Experiment 2
        5.2.4 Experiment 3
        5.2.5 Sample collection and Detection
        5.2.6 Histopathological examination
    5.3 RESULTS
        5.3.1 Experiment 1
        5.3.2 Experiment 2
        5.3.3 Experiment 3
    5.4 DISCUSSION
CHAPTER 6 VIRULENCE SCREENING OF M. hyorhinis STRAINS THROUGH MORTALITY INDEX OFEXPERIMENTALLY INFECTED CHICKEN EMBRYOS
    6.1 INTRODUCTION
    6.2 MATERIALS AND METHODS
        6.2.1 Quantification of the strains by CCU
        6.2.2 Inoculation
        6.2.3 Detection by Culture,PCR and Histopathology
        6.2.4 Statistical analysis
    6.3 RESULTS
        6.3.1 Mortality
        6.3.2 Clinical Presentation
        6.3.3 Detection by Culture and PCR
        6.3.4 Histopathology
    6.4 DISCUSSION
REFERENCES
OVERALL CONCLUSION
INNOVATION
LIST OF PUBLICATIONS
ACKNOWLEDGEMENTS
ABBREVIATIONS
APPENDIX


【參考文獻(xiàn)】：
期刊論文
[1]豬鼻支原體vlp重復(fù)區(qū)融合蛋白的構(gòu)建表達(dá)及反應(yīng)原性分析[J]. 熊祺琰,紀(jì)燕,劉占軍,馬慶紅,馮志新,劉茂軍,白方方,邵國青.  中國人獸共患病學(xué)報(bào). 2013(09)
[2]Identification of antigens by monoclonal antibody PD4 and its expression in Escherichia coli[J]. Jin-Ying Ning Guo-Xun Sun Su Huang Hong Ma Ping An Lin Meng Shu-Mei Song Jian Wu Cheng-Chao Shou, Department of Biochemistry and Molecular Biology, School of Oncology and Beijing Institute for Cancer Research, Peking University, Beijing 100034, China.  World Journal of Gastroenterology. 2003(10)



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