【摘要】：為篩選出抗傳染性支氣管炎病毒(IBV)效果較好的植物,試驗(yàn)采用嵌合熒光素酶基因的IBV-3ab-luc為供試毒株,以人類肺癌細(xì)胞H1299為供試細(xì)胞,在升麻、丹皮、千里光、續(xù)斷、元胡、龍葵6種植物乙醇提取物處理H1299細(xì)胞之前、中、后的一定時間感染,24 h后通過檢測熒光素酶值,研究不同植物乙醇提取物的抗病毒機(jī)制。結(jié)果顯示:丹皮的對數(shù)值為3,千里光的對數(shù)值為2,龍葵的對數(shù)值為1,升麻、續(xù)斷和元胡的對數(shù)值為0。說明丹皮乙醇提取物抗IBV活性最強(qiáng),其次是千里光,龍葵較差,而升麻、續(xù)斷和元胡幾乎沒有抑制效果。
[Abstract]:In order to screen out the plants with good anti-infectious bronchitis virus (IBV), the chimeric luciferase gene IBV-3ab-luc was used as the test strain, and the human lung cancer cell line H1299 was used as the test cell. The luciferase value of H1299 cells was detected 24 hours after treatment with ethanol extract from six plants, namely, Gastrodia elata, Radix Corydalis and Solanum nigrum, before, during and after the treatment of H1299 cells with ethanol extract of Radix Euphorbia pulcherriae and Solanum nigrum, and the luciferase value of H1299 cells was detected 24 hours later. The antiviral mechanism of ethanol extract from different plants was studied. The results show that the logarithmic value of Danpi is 3, the logarithmic value of Qianli light is 2, the logarithmic value of sunflower is 1, and the logarithmic value of Gastrodia elata, Tripterygium wilfordii and Yuanhu is 0. The results showed that the anti-IBV activity of Danpi ethanol extract was the strongest, followed by Qianliguang and Solanum nigrum, while Gastrodia elata, Tripterygium wilfordii and Radix Bupleurum had little inhibitory effect.
【作者單位】： 長江大學(xué)農(nóng)學(xué)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(31572490)
【分類號】：S853.7

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