【摘要】：恩拉霉素是由放線菌(Streptomyces fungicidicus)發(fā)酵而得的一類多肽類抗生素,由于其獨特的分子結構,對革蘭氏陽性菌有很強的活性,特別是對腸道內(nèi)的有害梭菌有很強的抑殺作用。作為全新的飼料添加劑,恩拉霉素能促進豬、雞等的生長,廣泛用于畜牧業(yè)。目前恩拉霉素的含量測定是按照農(nóng)業(yè)部1999年頒發(fā)的《進口獸藥質量標準》方法進行,本研究根據(jù)恩拉霉素的結構特點,通過實驗,建立了恩拉霉素的高效液相色譜檢測法。篩選優(yōu)化色譜條件為:色譜柱:Thermo C18,5μm,4.6mm×250mm;流動相:乙腈:0.05mol/L磷酸二氫鈉溶液=30:70;紫外檢測波長267nm;柱溫:30℃;流速:1.0mL/min;進樣量:20μL。在該條件下,檢測結果顯示恩拉霉素A和恩拉霉素B在12.5~200μg/mL范圍內(nèi)線性關系良好,恩拉霉素A和恩拉霉素B的回歸方程分別為A1=11587x+18926(R2=0.998)和A2=35969x-46745(R2=0.996);恩拉霉素A的平均回收率為98.9%,恩拉霉素B的平均回收率為98.7%;相對標準偏差(RSD)為0.27%。實驗結果證明采用建立的高效液相色譜法(HPLC)檢測恩拉霉素含量具有線性范圍寬、分析時間短、樣品前處理簡便、定量結果準確、重復性好、穩(wěn)定性好和回收率高等特點,可以作為恩拉霉素含量檢測的質控方法。同時將高效液相色譜法與現(xiàn)行標準微生物檢測法進行了比較,進一步證明高效液相色譜法(HPLC)操作簡便、快速、精準的特點,為其質量控制提供了更為可靠的依據(jù)。
[Abstract]:Enramycin is a kind of polypeptide antibiotics fermented by actinomycetes (Streptomyces fungicidicus). Because of its unique molecular structure, it has strong activity against Gram-positive bacteria, especially against harmful Clostridium perfringens in intestinal tract. As a new feed additive, Enramycin can promote the growth of pigs, chickens and so on, and is widely used in animal husbandry. At present, the content of Enramycin was determined according to the quality Standard of imported Veterinary drugs issued by the Ministry of Agriculture in 1999. according to the structural characteristics of Enramycin, a high performance liquid chromatography (HPLC) method for the determination of Enramycin was established. The optimum chromatographic conditions were as follows: chromatographic column: Thermo C 18, 5 渭 m, 4.6mm 脳 250 mm, mobile phase: acetonitrile: 0.05mol/L sodium dihydrogen phosphate solution = 30 鈮，

本文編號：2506583