【摘要】：建立山羊流產(chǎn)嗜性衣原體與Q熱立克次氏體基因組DNA特異擴(kuò)增檢測方法,對云南省河谷地區(qū)可疑流產(chǎn)病料時(shí)行病原檢測并最終證實(shí)病原的存在,在此基礎(chǔ)上了解病原的進(jìn)化。獲得特異基因產(chǎn)物完成測序驗(yàn)證后遞交GenBank,能過BLAST初步分析后調(diào)取相關(guān)基因,應(yīng)用DNAstar5.0計(jì)算序列間的相似性,計(jì)算遺傳距離,應(yīng)用MEGA5.2軟件進(jìn)行比對并構(gòu)建系統(tǒng)發(fā)育樹,進(jìn)行聚類分析。云南流產(chǎn)嗜性衣原體KR778870與有參考序列的衣原體毒株比較其核苷酸同源性均為100%,屬同一基因型,與鸚鵡熱衣原體同源率最高為94.9%;Q熱立克次氏體云南毒株KR697576與納米比亞毒株CP007555同源性為100%,其次美國毒株為CP00102同源率99.7%,臺(tái)灣株EU000273為99.4%,同源率最低的毒株EU009657僅為42.6%。通過基因特異性PCR確定流產(chǎn)山羊中檢測到流產(chǎn)嗜性衣原體及Q熱立克次氏體,流產(chǎn)嗜性衣原體PMP基因無變異,Q熱立克次氏體TransⅢ基因則有一定的變異。
[Abstract]:A specific amplification method of genomic DNA between chlamydia and Q Rickettsia in goat abortion was established. The pathogen was detected in suspected abortion materials in the valley of Yunnan Province and the existence of the pathogen was finally confirmed. On this basis, the evolution of the pathogen was understood. The specific gene products were sequenced and verified by GenBank, and the related genes were obtained after BLAST preliminary analysis. DNAstar5.0 was used to calculate the similarity between sequences, genetic distance was calculated, MEGA5.2 software was used to compare and construct the phylogenetic tree, and cluster analysis was carried out. Compared with chlamydia strains with reference sequence, the homology of KR778870 was 100%, belonging to the same genotyping, and the highest homology rate with Chlamydia psittaci was 94.9%, the highest homology rate was 94.9%, the homology rate of KR697576 was 100%, that of American strain was 99.7%, that of Taiwan strain was 99.4%, and that of EU009657, which had the lowest homology rate, was only 42.6%, which was 99.7% for the American strain, 99.4% for Taiwan strain, and 42.6% for the lowest homology rate of Chlamydia psittaci. By gene-specific PCR, chlamydia and Q-Rickettsia were detected in aborted goats. There was no variation in PMP gene of Chlamydia chlamydia abortion, but there was a certain variation in Q-Rickettsia TransIII gene.
【作者單位】： 玉溪農(nóng)業(yè)職業(yè)技術(shù)學(xué)院畜牧獸醫(yī)系;云南省熱帶亞熱帶動(dòng)物病毒病重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(31160499)
【分類號】：S858.27

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