不同石漠化程度對灌叢草地土壤細菌遺傳多樣性的影響
[Abstract]:In order to study the effect of lithodesertification on genetic diversity of soil bacteria in scrub grassland, potential (A), mild (B), was collected in the control area of lithodesertification in Puding County, Guizhou Province. Four different types of scrub grassland soil samples from moderate (C) and severe lithodesertification (D) were used as control (E), to study the structure of soil bacterial community by using PCR-DGGE technique. The results showed that the Shannon index and richness index of sample plot A were higher than those of sample plot B, C and D, indicating that the complexity and richness of bacterial community decreased with the aggravation of rocky desertification, while the (E) of control group was the lowest. This indicated that the complexity and richness of bacteria in the soil decreased significantly after farming. Cluster analysis showed that the control group E was a family, A, B, C, D was a large family, in which A and B, C and D soil bacterial PCR-DGGE map similarity was higher. In the analysis of phylogenetic tree, 13 sequence fragments were found to form 10 branches, and soil bacteria formed different community structure and high genetic diversity in the soil of different degrees of Rocky desertification scrub grassland.
【作者單位】: 貴州省草業(yè)研究所;
【基金】:貴州省科學技術(shù)基金(黔科合J字[2012]2198) 貴州省聯(lián)合基金(黔科合LH字[2014]7699)資助
【分類號】:S812.2
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