【摘要】：本試驗旨在建立單胃動物仿生消化系統(tǒng)模擬豬飼料原料消化后還原糖釋放量的測定方法,為飼料養(yǎng)分生物學效價的評估提供參考。還原糖釋放量與上樣量的線性關系研究以玉米-豆粕混合物(75%玉米+25%豆粕)為研究對象,上樣量設0.2、0.4、0.6、0.8和1.0 g 5個處理,每個處理4個重復,每個重復1根消化管;方法的重復性檢驗以玉米-豆粕混合物、大麥、花生粕和米糠為研究對象,每個樣品設3個批次,每個批次4個重復;方法的可加性檢驗設19個處理,其中處理1~7分別為玉米、大麥、高粱、大豆粕、花生粕、棉籽粕和米糠,處理8~19為2種或2種以上飼料原料按不同比例組合制備的12種混合飼料,每個處理4個重復,每個重復1根消化管,在仿生消化系統(tǒng)中模擬豬消化后測定各處理的還原糖釋放量。結果表明:上樣量在0.2~0.8 g時,還原糖總釋放量與上樣量呈顯著的線性關系(R2=0.999 2),還原糖相對釋放量在559.56~582.70 mg/g DM變化,變異系數(shù)為1.66%,而上樣量為1.0 g時,還原糖相對釋放量比上樣量為0.2~0.8 g時的平均值下降5.37%;3個批次的大麥、花生粕、米糠和玉米-豆粕混合物的還原糖釋放量的批內變異系數(shù)、批間變異系數(shù)和總變異系數(shù)均不大于1.68%,批間最大相對偏差分別為0.68%、1.50%、1.39%和0.29%;12種混合飼料還原糖釋放量的實測值顯著高于計算值(P0.05),而還原糖釋放量的計算值與實測值的線性回歸模型與y=x相重合(截距P=0.480 5;斜率P=0.514 1)。由此得出,當上樣量在0.2~0.8 g時,上樣量與還原糖釋放量呈顯著線性關系;仿生消化法測定豬飼料原料還原糖釋放量的重復性和可加性滿足定量分析的基本要求。
[Abstract]:The purpose of this study was to establish a method for the determination of reducing sugar release from pig feed after digestion by bionic digestive system of monogastric animals, and to provide a reference for the evaluation of the biological titer of feed nutrients. The linear relationship between reduced sugar release and sampling volume was studied by using corn-soybean meal mixture (75% corn 25% soybean meal) as the research object. The sample size was 0.2, 0.4, 0.6, 0.8 and 1.0 g, respectively, with 4 repeats in each treatment. One digestive tube per repetition; The reproducibility test of corn-soybean meal mixture, barley, peanut meal and rice husk was carried out. Each sample was divided into 3 batches and 4 repeats per batch. The additivity of the method was determined by 19 treatments, including corn, barley, sorghum, soybean meal, peanut meal, cottonseed meal and rice husk, respectively. Treatment 8 / 19 is 12 kinds of mixed feed prepared by combination of 2 or more feed materials in different proportion, with 4 repeats per treatment and 1 digestive tube per repetition. The release of reducing sugar from each treatment was measured in bionic digestion system. The results showed that when the sample size was 0.2 鈮，

本文編號：2486462