【摘要】：本試驗旨在研究飼糧中添加亞麻籽和魚油對蛋雞蛋黃n-3多不飽和脂肪酸含量、肝臟脂肪酸組成及其合成代謝基因表達(dá)的影響。選擇28周齡海蘭褐殼蛋雞96只,隨機分為4組,每組24只。對照組飼喂基礎(chǔ)飼糧,試驗組分別在基礎(chǔ)飼糧中添加10%亞麻籽、10%亞麻籽+5%魚油和5%魚油。飼養(yǎng)21 d后連續(xù)收集7 d雞蛋,然后每組隨機選取4只雞屠宰取樣。結(jié)果表明,與對照組相比:1)添加亞麻籽和魚油均顯著增加蛋黃中n-3多不飽和脂肪酸含量(P0.05),其中單獨添加魚油的蛋黃中二十二碳六烯酸(DHA)和二十碳五烯酸含量最高;2)試驗組肝臟單不飽和脂肪酸比例均顯著降低(P0.05),肝臟n-3多不飽和脂肪酸比例均顯著增加(P0.05);3)單獨添加魚油顯著降低肝臟脂肪酸延長酶1、脂肪酸延長酶2和去飽和酶(脂肪酸去飽和酶1、脂肪酸去飽和酶2和硬脂酰輔酶A去飽和酶1)基因表達(dá)水平(P0.05)。由此可見,蛋雞飼糧中單獨添加亞麻籽或魚油可以富集蛋黃n-3多不飽和脂肪酸沉積;添加亞麻籽促進(jìn)肝臟α-亞麻酸轉(zhuǎn)化生成DHA過程,表現(xiàn)為上調(diào)脂肪酸延長酶和去飽和酶基因表達(dá),添加魚油顯示相反結(jié)果。
[Abstract]:The purpose of this study was to study the effects of flaxseed and fish oil on the content of polysaturated fatty acids in egg yolk, the composition of fatty acids in liver and the expression of synthetic metabolic genes in laying hens. 96 28-week-old Hailan brown shell laying hens were randomly divided into 4 groups with 24 hens in each group. The control group was fed with basic diet, and the experimental group was fed with 10% flaxseed, 10% flaxseed 5% fish oil and 5% fish oil, respectively. Eggs were collected for 7 days after 21 days of feeding, and then 4 chickens in each group were randomly selected for slaughtering and sampling. The results showed that compared with the control group, 1) adding flaxseed and fish oil significantly increased the content of n 鈮，

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