【摘要】：本試驗旨在研究白酒糟(產(chǎn)自貴州美酒河流域)對黔北麻羊瘤胃發(fā)酵及血漿生化指標的影響。以6只年齡、體重基本一致的黔北麻羊為試驗動物,采用3×3拉丁方試驗設計,將其分成3組,每組2個重復,每個重復1只羊。對照組飼喂基礎飼糧,試驗1組和試驗2組以白酒糟分別替代基礎飼糧中20%、40%的精料。試驗期45 d,分3期,每期15 d,包括10 d預試期和5 d正試期。正試期采集血漿和瘤胃液樣品,測定瘤胃液pH、緩沖力、氨態(tài)氮和揮發(fā)性脂肪酸濃度,瘤胃液微晶纖維素酶、羧甲基纖維素酶、纖維二糖酶、木聚糖酶活性以及血漿葡萄糖、尿素氮、總蛋白、白蛋白、總膽固醇、肌酐含量。結果顯示:1)3組黔北麻羊瘤胃液pH、緩沖力差異不顯著(P0.05);氨態(tài)氮濃度均處于正常范圍,試驗1組氨態(tài)氮濃度顯著高于試驗2組(P0.05)。2)試驗1組瘤胃液木聚糖酶活性顯著高于對照組(P0.05),各組間瘤胃液微晶纖維素酶、羧甲基纖維素酶、纖維二糖酶活性差異不顯著(P0.05)。3)試驗1組瘤胃液乙酸濃度最高,顯著高于試驗2組(P0.05);瘤胃液總揮發(fā)性脂肪酸濃度也最高,顯著高于對照組和試驗2組(P0.05);3組瘤胃液丙酸和丁酸濃度、乙酸/丙酸差異均不顯著(P0.05)。4)各組血漿生化指標無顯著差異(P0.05)。由此可見,白酒糟替代40%以內(nèi)精料不會對黔北麻羊瘤胃發(fā)酵與血漿生化指標產(chǎn)生不良影響,相比較而言,以20%水平較優(yōu)。
[Abstract]:The purpose of this study was to study the effects of liquor grains (produced in Meijiuhe River Basin, Guizhou Province) on rumen fermentation and plasma biochemical indexes of Ma sheep in northern Guizhou. Six Qianbei Ma sheep, which were of the same age and weight, were divided into three groups with 2 repeats and 1 sheep in each group by 3 脳 3 Latin square design. The control group was fed with basic diet, and group 1 and group 2 were treated with liquor grains instead of 20% and 40% concentrate respectively. The trial period was 45 days, divided into 3 phases, 15 days each, including 10 days pre-trial period and 5 days positive trial period. Plasma and rumen juice samples were collected during the positive trial period to determine the pH, buffer capacity, ammonia nitrogen and volatile fatty acid concentrations, microcrystal cellulase, carboxymethyl cellulase, cellobiase, xylanase activity and plasma glucose in rumen fluid. Urea nitrogen, total protein, albumin, total cholesterol, creatine content. The results showed that: 1) there was no significant difference in pH, buffer capacity between the three groups (P 0.05). The concentration of ammonia nitrogen in group 1 was significantly higher than that in group 2 (P 0.05). 2) the activity of xylanase in rumen fluid in group 1 was significantly higher than that in control group (P 0.05). There was no significant difference in the activities of Carboxymethyl cellulase and cellulase (P 0.05). 3) the concentration of acetic acid in rumen juice of group 1 was the highest, which was significantly higher than that of group 2 (P 0.05). The concentration of total volatile fatty acids in rumen fluid was also the highest, which was significantly higher than that in control group and test group 2 (P 0.05). There was no significant difference in propionic acid and butyric acid concentration and acetic acid / propionic acid between the three groups (P 0.05). 4) there was no significant difference in plasma biochemical indexes among the three groups (P 0.05). It can be seen that the substitution of liquor grains for less than 40% concentrate will not have a negative effect on rumen fermentation and plasma biochemical indexes of Qianbei Ma Sheep, and the level of 20% is better than that of 20%.
【作者單位】： 貴州大學動物科學學院;貴州大學新農(nóng)村發(fā)展研究院;貴州省草業(yè)研究所;黔東南民族職業(yè)技術學院;
【基金】：國家自然科學基金(31360563) 貴州山區(qū)牧草產(chǎn)業(yè)化生產(chǎn)技術研究集成與應用(黔科合重大專項字[2014]6017號) 貴州大學科技興村人才培育基地建設項目;黔東南科合J字[2016]015號
【分類號】：S827.5

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