【摘要】：本研究以黔北麻羊為主要研究對象,分析FSHβ、FSHR、Gn RH和Gn RHR基因多態(tài)性與繁殖力的關系,Gn RHR和FSHR在發(fā)情間期和發(fā)情期的表達規(guī)律,Gn RHR基因CDS區(qū)功能及結構。本試驗旨在研究黔北麻羊繁殖相關基因多態(tài)與表達在低產(chǎn)和高產(chǎn)山羊的差異,為從分子遺傳方法上提高山羊繁殖力提供科學依據(jù)。研究結果如下:1、FSHβ和Gn RH基因中沒有檢測出多態(tài)位點,均不具有多態(tài)性。2、FSHR基因外顯子1的C126T和第10外顯子的C1246A和C1412A發(fā)現(xiàn)3個SNP位點。遺傳變異與產(chǎn)羔數(shù)關聯(lián)分析,C1246A位點,黔北麻羊個體產(chǎn)羔數(shù)存在差異,C1412A位點黔北麻羊和貴州黑山羊個體產(chǎn)羔數(shù)存在差異;在Gn RHR基因第1外顯子發(fā)現(xiàn)G121A突變。分析發(fā)現(xiàn),在黔北麻羊和貴州黑山羊兩個群體個體的產(chǎn)羔數(shù)存在明顯差異。3、在發(fā)情間期,黔北麻羊BB基因型個體下丘腦和子宮FSHR的表達量高于AA基因型個體(P㩳0.05);黔北麻羊AA基因型垂體、輸卵管、卵巢和子宮Gn RHR的表達量高于GG基因型黔北麻羊(P㩳0.05)。在發(fā)情期,黔北麻羊BB基因型個體卵巢和子宮FSHR的表達量高于AA基因型個體(P㩳0.05);黔北麻羊AA基因型垂體、輸卵管、卵巢和子宮Gn RHR的表達量高于GG基因型黔北麻羊(P㩳0.05)。4、在黔北麻羊垂體中成功克隆Gn RHR基因CDS區(qū)984bp,可編碼328個氨基酸。
[Abstract]:In this study, Guizhou Ma sheep was used as the main research object to analyze the relationship between FSH 尾, FSHR,Gn RH and Gn RHR gene polymorphism and fecundity. The expression of, Gn RHR and FSHR during estrus and estrus was analyzed. The function and structure of, Gn RHR gene CDS region. The purpose of this study was to study the difference between the polymorphism and expression of reproductive related genes in low yield and high yield goats, and to provide scientific basis for improving the fecundity of goats by molecular genetic method. The results were as follows: 1. No polymorphism was detected in Gn RH 尾 and gene. 2, three SNP loci were found in C126T in Exon 1 and C1246A and C1412A in Exon 10. The association analysis between genetic variation and lambing number showed that there were differences in C1246A locus and individual lambing number between Guizhou and Guizhou black sheep, and G121A mutation was found in exon1 of Gn RHR gene in Guizhou black sheep and Guizhou black sheep, and there were differences in individual lambing number between Guizhou black sheep and Guizhou black sheep at C1246A locus and individual lambing number in Qianbei Ma sheep and Guizhou black sheep at C1412A locus. It was found that there was a significant difference in the number of lambs between Guizhou black sheep and Guizhou black sheep. 3, in the estrus period, The expression of FSHR in Hypothalamus and uterus of individuals with BB genotype was higher than that of individuals with AA genotype (P 鈮，

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