【摘要】：試驗(yàn)旨在通過有益菌轉(zhuǎn)化無機(jī)鋅制備富鋅益生素。將鋅濃度為50 mg/ml的硫酸鋅溶液與產(chǎn)朊假絲酵母、嗜酸乳桿菌、凝結(jié)芽孢桿菌種子液混合發(fā)酵培養(yǎng),研究不同溫度、培養(yǎng)時間、鋅添加量、接種量、烘干溫度對富鋅益生素活菌數(shù)及鋅轉(zhuǎn)化率的影響。結(jié)果表明:硫酸鋅溶液和復(fù)合菌固體發(fā)酵培養(yǎng)基中比例為1:200,混合種子液接種量比例為5%;發(fā)酵溫度35℃;培養(yǎng)時間為36 h;烘干溫度為60℃;得到的富鋅益生菌總活菌數(shù)9.07×10~9 CFU/g,有機(jī)鋅轉(zhuǎn)化率為79.82%。用10倍劑量的富鋅益生菌連續(xù)飼喂雛雞5周,雛雞健康狀況良好。
[Abstract]:The purpose of the experiment was to prepare zinc-rich probiotics by transforming inorganic zinc into beneficial bacteria. The zinc sulfate solution with zinc concentration of 50 mg/ml was mixed with Pseudomonas aeruginosa, Lactobacillus aeruginosa and Bacillus cloacae seed liquid. The different temperature, culture time, zinc addition and inoculum size were studied. Effect of drying temperature on the number of zinc-rich probiotics and zinc conversion. The results showed that the proportion of zinc sulfate solution and compound bacteria in solid fermentation medium was 1 鈮，

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